Real-Time PCR

Highly efficient Applied Biosystems real-time PCR solutions minimize complexities to help maximize your time and effort

Real-time PCR, also known as quantitative PCR (qPCR), is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets. We developed powerful assay design algorithms, optimized master mixes, created intuitive data analysis software, and built smart instrumentation to help harness the power of real-time PCR across a rich and diverse set of applications. However, our solutions go a step further: every product has been designed with efficiency baked in. And together they work synergistically to bring you a simpler, more flexible, and highly efficient real-time PCR workflow.

Step into the future of molecular diagnostics

Take command of testing with innovative qPCR solutions.

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qPCR solutions for your BioPharma workflow

Give your biologics development an edge

Streamline your qPCR workflow with QualTrak solutions.

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Research solutions

QuantStudio real-time PCR systems

With more than 25 years of qPCR innovation, Applied Biosystems continues to lead the way in performance, reliability, and world-class support. From low- to high-throughput solutions, there’s a QuantStudio Real-time PCR system that’s just right for your research.

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TaqMan real-time PCR assays

Industry-leading choice for 5ʹ nuclease real-time PCR assays. >20 million predesigned assays (primer/probe sets) available in five formats and unlimited content configurations. Custom designs and individual primers/probes also available.

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Real-time PCR reagents & kits

TaqMan master mixes are optimized to work with our TaqMan Assays to provide optimal sensitivity, specificity, and dynamic range. Our SYBR portfolio offers a variety of features providing a wide range of instrument compatibility.

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Real-time PCR data analysis

From gene expression to Ct analysis and copy number variation determination, we have developed robust analysis software for nearly every real-time PCR application. Plus, all our analysis tools have been optimized for use with our TaqMan Assays on Applied Biosystems Real-time PCR instruments.

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Real-time PCR applications

Gene expression (mRNA) analysis

Detect and quantifying target genes, even at very low expression levels.

MicroRNA (miRNA) analysis

Solutions for miRNA research. miRNAs are small, noncoding RNA molecules that direct post-transcriptional suppression of gene expression.

Genetic variation analysis

Genetic variation refers to the differences in the DNA sequence encoded within our genomes. Specific applications include:

Protein expression analysis

TaqMan Protein assays combine the protein-binding of antibodies with the quantitation of real-time PCR. They enable fast, easy identification of proteins from small samples in a homogeneous assay format.

Mutation detection

Real-time PCR is a widely used technology for mutation detection that provides sensitivity and specificity when paired with TaqMan Assays and is fast and easy to perform compared to other methods. Explore these solutions:

Verification of NGS and microarray results

Together, RNA-Seq, microarrays, and qPCR provide a comprehensive workflow for gene expression analysis, from discovery and testing to downstream verification.

Explore all qPCR application areas