BPAE imaging using CellROX® Deep Red Reagent (Cat. No. C10422)

Bovine pulmonary artery endothelial (BPAE) cells were plated in a 96-well plate. BPAE cells were treated with or without 100 µM menadione for 1hour at 37°C. 100 µM superoxide scavenger, MnTBAP, was added to some of the control and menadione-treated wells for the last 30 minutes of incubation. The cells were then stained with 5 µM CellROX® Deep Red Reagent and Hoechst 33324 by adding the probe to the complete medium and incubating the cells at 37°C for 30 minutes. The cells were then washed with PBS and imaged.

Bovine pulmonary artery endothelial (BPAE) cells were plated in a 96-well plate. BPAE cells were treated with or without 100 µM menadione for 1hour at 37°C. 100 µM superoxide scavenger, MnTBAP, was added to some of the control and menadione-treated wells for the last 30 minutes of incubation. The cells were then stained with 5 µM CellROX® Deep Red Reagent and Hoechst 33324 by adding the probe to the complete medium and incubating the cells at 37°C for 30 minutes. The cells were then washed with PBS and imaged.

Related Products

Related Images

Oxidative stress analysis in BPAE and Raw cells using CellROX® Deep Red Reagent (Cat. No. C10422) Go ›

BPAE detection of oxidative stress using MitoTracker® Green (Cat. No. M7514) and CellROX® Deep Red Reagent (Cat. No. C10422) Go ›

Live cell imaging with CellLight™ reagents. Go ›

Live cells transduced with Organelle Lights™ or Cellular Lights™ reagents. Go ›

CD335 (NKp46) Antibody (63335182) in RE Go ›

CD223 (LAG-3) Antibody (56223942) in TM Go ›