Having difficulties with your experiment?

We are dedicated to your success. Get back on track. View our expert recommendations for commonly encountered problem scenarios with your animal health disease detection experiments.

View the relevant questions below:

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General

I think I am getting inhibition in my qPCR results? How can I test this?

You can try diluting your nucleic acid samples and see if your qPCR signal gets stronger. Alternatively, you can use an extraction control such as the VetMAX Xeno Internal Positive Control we offer (Cat. Nos. A29763, A29764). 

My Xeno control assay failed. What are some possible causes?

If your Xeno control fails, you could possibly have inhibitors in your samples. You can test this by running the Xeno control directly in a qPCR reaction. You can also run a mock extraction control where you spike the Xeno control into a buffer sample and then perform the nucleic acid extraction procedure you used for your samples. 

What can I do if the amplification of my target gene is later than expected?

There are several reasons that amplification could be delayed. Please see the information in our real-time PCR troubleshooting tool for more details.

My amplification curves have a funny shape. What is causing this?

If your amplification curves do not have a normal appearance, you may be having an issue with the baseline settings. Please see these examples and suggested solutions in our real-time PCR troubleshooting tool for more details.

I am getting amplification in my no-template control (NTC) wells. What is causing this?

There are several reasons for amplification in a NTC well. Please see the information in our real-time PCR troubleshooting tool for more details.

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