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The Invitrogen Qubit 4 Fluorometer accurately and quickly measures the concentration of DNA, RNA, or protein in a single sample. It can also be used to assess RNA integrity and quality. The easy-to-use touchscreen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.
The Qubit 4 Fluorometer shares common features with the Qubit Flex model: It is accurate, specific, sensitive, simple, and fast; it requires as little as 1 µL of sample, even if highly diluted; it offers an onboard Reagent Calculator to help in preparing your working solution; it works with a broad range of Qubit Assays and applications; and it can measure raw fluorescence in Fluorometer mode.
In addition, data gathered in Fluorometer mode on the Qubit 4 Fluorometer can be used to design your own assay using the MyQubit assay design tool. This tool lets you create new assays for the Qubit Fluorometer in minutes. Simply enter your assay parameters into the online tool, and then save and upload the .qbt file to your Qubit Fluorometer using a USB drive.
Preconfigured MyQubit assays to quantify cholesterol, galactose, glucose, glutamic acid, peroxide, or sucrose are available. You can download these .qbt files to your computer and then upload them to your Qubit 4 Fluorometer using a USB drive. These are examples of the kinds of MyQubit assays you can create on your own.
The user-friendly Qubit 4 Fluorometer delivers results using quick, simple assay procedures. The large, color touch screen allows you to easily navigate through the various options. Calculations and settings are automatically performed by the instrument.
The Qubit 4 Fluorometer will save the data for up to 1,000 samples, and you can transfer data to a Thermo Fisher Connect Cloud account via WiFi, or to a computer via a USB drive or cable. In addition, the Qubit 4 integrated Reagent Calculator helps you determine the amount of dye and buffer required to prepare your samples and standards.
Reagent Calculator and results graph. Enter the number of samples and standards needed and the built-in Reagent Calculator will determine the amount of dye and buffer needed. The results can be viewed on a graph showing the standard curve.
Attribute |
Description |
Physical characteristics |
|
Instrument type |
Benchtop fluorometer |
Instrument dimensions |
13.6 x 25 x 5.5 cm 5.4 x 10 x 2.2 in. |
Weight |
743 g; 2.1 lb |
Operating power |
100-240 VAC, 1.0 A/1.2 A |
Frequency |
50/60 Hz |
Electrical input |
12 VDC, 2.5 A |
Installation site |
Indoor use only |
Operating temperature |
10-40° C |
Operating humidity |
20-80% (non-condensing) |
User interface |
5.7-inch color touchscreen |
Technical specifications |
|
Dynamic range |
5 orders of magnitude |
Processing time |
<5 seconds/sample |
Light sources |
Blue LED (peak ~470 nm) Red LED (peak ~635 nm) |
Excitation filters |
Blue LED (430–495 nm) Red LED (600–645 nm) |
Emission filters |
Green (510–580 nm) Red (665–720 nm) |
Detectors |
Photodiodes: measurement capability from 300–1,000 nm |
Calibration type |
2- or 3-point standard |
Sample chamber |
Accommodates one Qubit Assay Tube |
Tube type |
Qubit Assay Tube |
Warm-up time |
<35 seconds |
Sample data storage |
1,000 samples |
Hardware |
|
Display |
5.7 in. capacitive touchscreen with high-resolution color display |
Data ports |
USB cable port (type mini-B) USB drive port (type A) |
Networking capabilities |
Transfer data directly to computer using a USB cable or wirelessly using the supplied Wi-Fi adaptor |
Power supply |
AC adapter with country-specific power cords |
USB drive |
4 GB |
Qubit 4 Fluorometer |
Qubit 4 Fluorometer |
Qubit 4 Fluorometer |
Qubit 4 Fluorometer |
Qubit 4 Fluorometer |
1 international power adapter |
1 international power adapter |
1 international power adapter |
1 international power adapter |
1 international power adapter |
Microfiber cloth |
Microfiber cloth |
Microfiber cloth |
Microfiber cloth |
Microfiber cloth |
USB drive |
USB drive |
USB drive |
USB drive |
USB drive |
WiFi dongle |
WiFi dongle |
WiFi dongle |
WiFi dongle |
WiFi dongle |
|
Qubit Assay Tubes (500 tubes) |
Qubit Assay Tubes (500 tubes) |
Qubit Assay Tubes (500 tubes) |
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Qubit dsDNA HS (high sensitivity) Assay Kit (100 assays) |
Qubit dsDNA HS (high sensitivity) Assay Kit (500 assays) |
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Qubit dsDNA BR (broad range) Assay Kit (100 assays) |
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Qubit RNA IQ (integrity and quality) Assay Kit (75 assays) |
|
Qubit RNA IQ (integrity and quality) Assay Kit (275 assays) |
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Qubit RNA HS (high sensitivity) Assay Kit (100 assays) |
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Qubit RNA BR (broad range) Assay Kit (100 assays) |
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Qubit Protein BR Assay Kit (100 assays) |
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Qubit Protein BR Assay Kit (100 assays) |
|
Qubit 4 System Verification Assay Kit (50 assays) |
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For information about assays for the Qubit 4 Fluorometer, see the Assays tab.
For Research Use Only. Not for use in diagnostic procedures.