Purificação e Análise de DNA e RNA

Nossos produtos de purificação de ácidos nucleicos são otimizados para fornecer o máximo de rendimento, pureza e integridade de, praticamente, qualquer tipo de amostra. Explore nossa linha de produtos Invitrogen para a purificação eficiente de RNA, miRNA, plasmídeos de DNA e gDNA. Conheça nossos inovadores produtos Invitrogen Cells-to-C_T CT para cobertura total de isolamento de ácidos nucleicos, bem como kits para trabalhar com amostras FFPE.

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Organic extraction and precipitation

Nucleic acid isolation by organic extraction involves addition of phenol and guanidine isothiocyanate to separate the DNA, RNA, and proteins into different organic phases. Organic extraction is a low-cost method, and with advanced reagents such as TRIzol, is a straightforward process requiring very little equipment.

Silica-based nucleic acid isolation

Nucleic acids bind to silica (also known as glass fibers) under high-salt conditions and can be released under low-salt conditions. Silica-containing columns provide an easy way to bind, wash, and elute purified nucleic acids from multiple clarified cell lysates in parallel.

Invitrogen Purelink and GeneJET columns are designed to flow buffers through centrifugation, vacuum, or gravity. Most protocols use spin column technology to take advantage of readily available lab equipment. Spin plates provide a high-throughput format based on the same isolation principle.

Paramagnetic bead-based nucleic acid purification

In this method paramagnetic (attracted to magnet) beads are added to the sample, and nucleic acids bind to the beads. Using a strong magnet, the beads are held in place while removing unwanted material. After washing, the genetic material is eluted from the beads in water or a low-salt buffer.

Magnetic bead isolation is now one of the most popular nucleic acid extraction methods due to its scalability and automation compatibility; MagMAX Bead Kits and KingFisher Sample Purification Systems are designed to work together to efficiently purify a variety of nucleic acids.

Our nucleic acid extraction instruments, kits and reagents are optimized to provide maximum yield, purity, and integrity from virtually any sample type. Whether you need to extract and purify RNA, viral nucleic acid, DNA fragments, plasmid DNA, or genomic DNA, Thermo Fisher Scientific has a solution.

Quantificação fácil e precisa de ácidos nucleicos com os fluorômetros Qubit e kits de ensaio Quant-iT.

Kits de limpeza simples e rápida de PCR e géis para purificar o DNA de seu interesse.