Evolution UV-Vis Spectrophotometers

Evolution UV-Vis Spectrophotometers

Transform your UV-Visible experience with Thermo Scientific Evolution Spectrophotometers. Simplified workflows, advanced technology, and an array of accessories consistently deliver the high-quality results you depend on.

 

From simple measurements to sophisticated research studies, Evolution Spectrophotometers deliver reliable, accurate results. The simple user interface prompts users through the workflow, and complete solutions enable compliance with regulatory requirements. 

Evolution UV-Vis Spectrophotometer features

Our next generation Evolution UV-Vis Spectrophotometers combine reliable and versatile hardware with easy-to-learn, easy-to-use Thermo Scientific Insight Pro Software. Together they will meet the diverse needs your organization requires from an analytical tool.

 

Meet today’s demands head-on with a solution-based approach

Reliable, versatile hardware

  • Address your laboratory’s needs with fixed or variable bandwidth options
  • Meets chemical, materials science, or biological sampling and measurement needs with a broad range of accessories, including those designed for previous Evolution models

A software partner

  • Meets current 21 CFR part 11 compliance, including database storage  (optional software)
  • Validate according to current USP, EP, or JP standards
  • Streamline your simple, complex, and advanced routines and workflows

Evolution UV-Vis Spectrophotometer selection guide

Evolution Spectrophotometers incorporate a high-performance optical design, versatile Insight Pro Software packages, and the highest quality accessories for the most demanding applications. 

 

Choose the instrument that’s right for you

 

  Evolution One Spectrophotometer Evolution One Plus Spectrophotometer Evolution Pro Spectrophotometer
 
  Routine measurements, high resolution data Greater experimental flexibility for research and more complex applications Greater experimental flexibility for research and more complex applications
Hardware
Spectral bandwidth Fixed: 1 nm Variable:1 nm, 2 nm
AFBG Microcell optimized;
AFBG Fiber optic optimized;
AFBG Materials optimized		 Variable: 0.5, 1, 1.5, 2, 4 nm
Light source Xenon Xenon Xenon
Optical system Double beam Double beam Double beam
Software
Current Pharmacopoeia compliance USP and EP USP and EP USP, EP, and JP
21 CFR part 11* ✓ ✓ ✓

CUE

(Customized User Environment software)

 ✓ ✓ ✓
Database storage ✓ ✓ ✓
Report printing ✓ ✓ ✓
Kinetics ✓ ✓ ✓
Featured accessory compatibility**
Mercury lamp ✓ ✓ ✓
pre-installed option also available
Fiber optics   ✓ ✓
Peltier and Sipper systems ✓ ✓ ✓
Multi-cell options ✓ ✓ ✓
Integrating sphere for transmittance and reflectance   ✓  
VeeMax® Variable Specular Reflectance       ✓
Praying Mantis Diffuse Reflectance     ✓
Warranty 2 years 2 years 2 years

 

*Requires Thermo Scientific Insight Pro Security Software

**Optional accessories are sold separately

Evolution UV-Vis Spectrophotometer specifications

Product

Evolution One

Evolution One Plus

Evolution Pro

Optical Design

Double-beam with sample and reference cuvette positions; Czerny-Turner monochromator

Double-beam with sample and reference cuvette positions; Application Focused Beam Geometry (AFBG); Czerny-Turner monochromator

Double-beam with sample and reference cuvette/accessory positions; Modified Ebert monochromator

Spectral Bandwidth

1.0 nm

Variable: 1.0 nm; 2.0 nm; AFBG Microcell optimized; AFBG Fiber optic optimized; AFBG Materials optimized

Selectable 0.5, 1.0, 1.5, 2.0, 4.0 nm

Light Source (Typical Lifetime)

Xenon flash lamp

(>5 years; longer if not using live display;
3-year source replacement warranty)
Detector Dual Silicon Photodiodes Detector dual-matched silicon photodiodes
Scan Ordinate Modes Absorbance
% Transmittance
% Reflectance
Kubelka-Munk
log (1/R)
log (Abs)
Abs*Factor
Intensity		 Absorbance
% Transmittance
% Reflectance
Kubelka-Munk
log (1/R)
 log (Abs)
Abs*Factor
Intensity
1st–4th Derivative
Grating Holographic, 1200 lines/mm, blazed at 250 nm Holographic, 1200 lines/mm, blazed at 240 nm
Wavelength Range 190–1100 nm
Accuracy

±0.5 nm (541.9 nm xenon, 546.1 nm mercury lines)

±0.8 nm (full range 190–1100 nm)

±0.20 nm (546.07 nm Hg emission line)

±0.30 nm (190–900 nm)
Repeatability

≤0.05 nm

(546.1 nm mercury line, SD of 10 measurements)
Scanning speed <1 to 6000 nm/min; variable Variable, up to 6000 nm/min
Data intervals 10, 5, 2, 1.0, 0.5, 0.2, 0.1 nm 10, 5, 2, 1, 0.5, 0.2, 0.1, 0.05 nm
Photometric Range >3.5 A >4 A
Display Range -0.3 to 4.0 A ±6 A
Accuracy—Instrument1

1A: ±0.004 A

2A: ±0.008 A

Measured at 440 nm using neutral density filters traceable to NIST

1A: ±0.004 A

2A: ±0.004 A

3A: ±0.006 A

Measured at 440 nm using neutral density filters

traceable to NIST
Repeatability1 1A: ±0.0002 A 1A: ±0.0001 A
Noise

0A: ≤0.00015 A

1A: ≤0.00025 A

2A: ≤0.00050 A

260 nm, 1.0 nm SBW, RMS

0A: <0.00018 A

1A: <0.00022 A

2A: <0.00050 A

500 nm, 2.0 nm SBW, RMS
Drift (Stability)

<0.0005 A/hour

500 nm, 1.0 nm SBW,
1 hour warm-up

<0.0005 A/hour

500 nm, 2.0 nm SBW,
2 hour warm-up
Stray Light

KCl, 198 nm: ≤1% T

NaI, 220 nm: ≤0.05% T

NaNO2, 340 nm: <0.05% T

KCl, 198 nm: ≤0.4% T

NaI, 220 nm: ≤0.032% T

NaNO2, 340 nm: <0.01% T
Baseline Flatness

±0.001 A (200–800 nm)

1.0 nm SBW, smoothed

±0.001 A (200–800 nm)

2.0 nm SBW, smoothed
Dimensions (W × D × H) 593 × 475 × 266 mm
(23.3" × 18.7" × 10.6")		 609 × 526 × 270 mm
(23.9" x 20.7" x 10.6")
Weight 14.5 kg (32 lb) 20 kg (44 lb)
Electrical supply 100–240 V, 50–60 Hz, selected automatically
150 W maximum		 100–240 V, 50–60 Hz
Additional Specifications

Typical specifications

Guaranteed specifications

Product specifications

1 When testing instrument performance, the specification used for pass/fail determination is the sum of the instrument specification listed here and the uncertainty in the calibration data for the filter, listed on the calibration certificate.

Evolution UV-Vis Spectrophotometer pharmacopeia compliance

For UV-Visible spectrophotometers in regulated laboratories, performance verification at installation and at set intervals thereafter is needed. National pharmacopeias with international reach define specific performance levels for various criteria in the pharmaceutical industry.

 

Note that Evolution One/One Plus models meet current USP and EP compliance and the Evolution Pro model meets current USP, EP, and JP compliance.

Parameters Standard(s) used Pharmacopeia specification (tightest)
Wavelength accuracy Mercury lamp USP - ≤ ± 1 nm in UV range ≤ ± 2 nm in visible range
JP - ≤ ± 0.3 nm over full λ range
Holmium perchlorate solution USP - ≤ ± 1 nm in UV range ≤ ± 2 nm in visible range
Didymium solution or glass USP - ≤ ± 1 nm in UV range ≤ ± 3 nm in visible range
JP - ≤ ± 0.5 nm over full λ range
Wavelength repeatability Mercury lamp JP - ≤ ± 0.2 nm over full λ range USP - ≤ ± 0.5 nm over full λ range
Holmium perchlorate solution EP/USP - ≤ ± 0.5 nm over full λ range
Didymium solution or glass JP - ≤ ± 0.2 nm over full λ range
Photometric accuracy Certified potassium dichromate (K2Cr2O7) EP - ± 0.010A USP - ≤ ± 0.010A in UV range ≤ ± 0.008A in visible range
Certified filters EP - ≥1.0A, ±0.8%A; <1.0A, ±0.008ª
JP - ± 1% of upper and lower limits of standard transmission rate value
Neutral density filters (Vis only) TGA
Photometric repeatability Certified potassium dichromate (K2Cr2O7) JP - For standard values ≤ 0.500, ±0.002 of mean; for standard values >0.500, ±0.004
Neutral density filters (Vis Only) JP - For standard values ≤ 0.500, ±0.002 of mean; for standard values >0.500, ±0.004
Stray light Certified potassium chloride (KCl, 12g/L) EP - > 2.0A @ 198 nm (< 1%T)
Potassium iodide or sodium iodide (KI/NaI, 10g/L) USP/EP - > 3.0A @ 220 nm
Acetone USP - > 0.7A @ 300 nm
Sodium nitrite (NaNO2, 50g/L) EP- > 3.0A @ 340 nm
Resolution Toluene in hexane (0.02%) Ratio @ 0.5 SBW ≥ 2.3 at 25 °C
Photometric linearity Certified filters JP/EP - Verify linearity of transmission rate, r2 > 0.999

 

Evolution UV-Vis Spectrophotometer ordering information

Description

Part Number

Evolution One UV-Vis Spectrophotometer

840-341400

Evolution One Plus UV-Vis Spectrophotometer

840-341500

Evolution Pro UV-Vis Spectrophotometer

840-340200

For Research Use Only. Not for use in diagnostic procedures.