Pre-Packed Chromatography Columns

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Pre-packed chromatography columns for bioprocess applications

Chromatography columns are critical to achieving successful separation of any biomolecule in downstream applications. Our pre-packed chromatography columns are designed to save time and money when used in your biotherapeutic purification workflow. We offer a variety of column sizes and formats, packed with Thermo Scientific chromatography resins. Our columns range in size from small volume RoboColumns, such as GoPure and MiniChrom™ columns used for process development, to bench scale.

No matter what your application is, we have a packed column ready when you are. Our pre-packed columns can help increase your productivity by:

Offering a quick and easy set-up
Allowing for increased process flexibility
Helping create efficiencies so there is more time for more test runs
Reducing labor time related to column packing and cleaning

Pre-packed chromatography columns for multiple applications

Our pre-packed columns come in a variety of formats, suitable for high throughput screening, process development, bench-scale screening, and small-scale preparative chromatography.

Column type	RoboColumn™			GoPure small-scale prepacked column		MiniChrom column		Prepacked column
Application	High throughput screening Process development			Process development Bench-scale screening Preparative chromatography		Process development Bench-scale screening Preparative chromatography		Preparative chromatography
Volume	50 µL	200 µL	600 µL	1 mL	5 mL	1 mL	5 mL	1 mL	5 mL
Diameter	0.5 cm	0.5 cm	0.5 cm	0.5 cm	0.8 cm	0.8 cm	0.8 cm	0.7 cm	1.4 cm
Bed height	0.25 cm	1 cm	3 cm	5 cm	10 cm	2 cm	10 cm	2.5 cm	3.25 cm

High throughput screening (Process development)

RoboColumns are small chromatography columns that are provided in 8-column strips. These columns are useful for fully automated and parallel chromatographic separations using a robotic liquid handling platform and are available in 50 µL, 200 µL, and 600 µL volumes.

Bench-scale process development screening

The GoPure and MiniChrom columns are designed for bench-scale resin screening, process development, and sample preparation. The 1 mL columns can be used for quick screenings of application feasibility and lab scale purification on a convenient and easy-to-use pre-packed column. The 10 cm bed height of the 5 mL columns allows initial process development on a bench scale.

Preparative chromatography

For your small-scale preparative chromatography needs, we have pre-packed our affinity resins into columns which can be used for simple and efficient protein purification in small volumes.

Find your column by resin type

Affinity

The CaptureSelect affinity chromatography technology offers a unique affinity purification solution based on single domain [VHH] antibody fragments. The affinity resins provide high target purity in a single step, independent of feedstock.

The affinity ligands are developed to meet all predefined requirements such as high specificity to the target molecule, mild elution during the purification process and high stability of the final resin. Manufacturing in an animal free production process allows for use in commercial purification processes

Product		RoboColumn (50 µL/200 µL/600 µL)	GoPure 0.2 or 1 mL / MiniChrom 1 mL	GoPure 5 mL/ MiniChrom 5 mL	pre-packed 1 mL	pre-packed 5 mL
Viral vectors	AAVX	A37794 (200 µL) A37795 (600 µL)	A36652	A36651
	AAV8		A36648	A36647
	AAV9		A36650	A36649

Product

RoboColumn

(50 µL/200 µL/600 µL)

GoPure 0.2 or 1 mL / MiniChrom 1 mL

GoPure 5 mL/ MiniChrom
5 mL

pre-packed 1 mL

pre-packed 5 mL

C-tagXL

Product		RoboColumn (50 µL/200 µL/600 µL)	GoPure 0.2 or 1 mL / MiniChrom 1 mL	GoPure 5 mL/ MiniChrom 5 mL	pre-packed 1 mL	pre-packed 5 mL
Antibodies

	FcXP	5943712200 (200 µL)	5943712001	5943712005	494371201	494371205
	CH1-XL	5943462200 (200 µL)	5943462001	5943462005	494346201	494346205
	KappaXP	5943212200 (200 µL)		5943212005	494321201	494321205
	LambdaXP		594375001	594375005
	MabCaptureC (Protein A)	5943662200 (200 µL) 5943662600 (600 µL)	5943662001	5943662005

Product

RoboColumn

(50 µL/200 µL/600 µL)

GoPure 0.2 or 1 mL / MiniChrom 1 mL

GoPure 5 mL/ MiniChrom
5 mL

pre-packed 1 mL

pre-packed 5 mL

mRNA

POROS Oligo (dT)25

A48349
(200 µL)

A48350
(600 µL)

A48351
(0.2 mL)

A48352
(1 mL)

A48607

Ion exchange

POROS Ion Exchange Resins are designed for high-dynamic binding capacity over a range of pH and conductivity conditions, thereby increasing process development flexibility and manufacturing throughput.

POROS HS and XS resins are strong cation exchange resins based on a sulfopropyl functionalization, designed for the purification of more basic proteins and biomolecules. They are especially well suited for the separation of process impurities and aggregates.

Resin type	Product	RoboColumn 200 µL	RoboColumn 600 µL	GoPure 1 mL	GoPure 5 mL
Anion	HQ 50	A30717	A30718	4481315	A36639
	XQ	A30719	A30720	A25812	A36640
	PI 50	A30721	A30722	4481318	A36641
	D 50	A30723	A30724	4481319	A36642

Resin type	Product	RoboColumn 200 µL	RoboColumn 600 µL	GoPure 1 mL	GoPure 5 mL
Cation	HS 50	A30713	A30714	4481316	A36637
Cation	XS	A30715	A30716	4481317	A36638

Hydrophobic interaction

POROS HIC resins are available in a differentiating range of hydrophobicity, suitable for bind/elute and flow-through applications. The resins can be utilized at lower salt concentrations for the purification of a wide variety of biomolecules including therapeutic proteins, antibody fragments, antibody drug conjugates (ADCs), and other large molecules.

Resin type	Product	RoboColumn 200 µL	RoboColumn 600 µL	GoPure 1 mL	GoPure 5 mL
HIC	Ethyl	A34810	A34812	A34983	A36653
	Benzyl	A34813	A34814	A34984	A36654
	Benzyl Ultra	A34815	A34816	A34985	A36655

Chromatography Learning Lab

Check out the new Chromatography Learning Lab from Thermo Fisher Scientific, providing a robust range of webinars, articles, eBooks, infographics, and other digital resources to help you optimize the use of our purification products in your process.

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