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Equipped with acoustic-assisted hydrodynamic focusing, the Invitrogen Attune Flow Cytometers are able to achieve sample-throughput rates up to 10 times faster than traditional cytometers. This means that you can process samples more quickly and acquire sufficient events even from very dilute samples. The Attune Flow Cytometers accommodate no-wash, no-lyse protocols, allowing you to collect data on samples that have been subjected to minimal handling. In addition, the Attune CytPix Flow Cytometer includes a high-speed, high-resolution, brightfield camera that records images of particles as they pass through the flow cell. The camera and Attune Cytometric Software help ensure that events recorded by the detector are single cells as opposed to doublets, clumps, or debris.
"We have yet to clog the machine with our debris-rich primary tumor samples. Of course, the acoustic technology greatly facilitates the identification of small populations, like cancer stem cells, increasing our capacity to detect and quantify these rare events with high efficiency and reliability."
– Bruno Sainz Jr., PhD, Autonoma University of Madrid, School of Medicine
This video demonstrates the event alignment that occurs when acoustic focusing is applied.
Equipped with acoustic-assisted hydrodynamic focusing, the Attune Flow Cytometers process samples faster than traditional cytometers. This breakthrough technology uses sound waves for more precise cell alignment and higher data quality. The pre-alignment of cells allows the Attune Flow Cytometers to process samples quickly and acquire more statistically significant events even from very dilute samples. Acoustic focusing is doubly useful for cellular imaging on the Attune CytPix instrument, helping to position the cells precisely so that a sharp image is obtained.
Flow cytometry data quality depends on collecting information from thousands of individual cells as they pass through the laser interrogation point. The acoustic technology within Attune Flow Cytometers generate ultrasonic waves that act to transport particles to the center of the sample stream. The pre-focused stream is injected into the sheath stream, which applies hydrodynamic pressure to the sample. The combination of these two forces results in a narrow core stream and an increased probability that cells are passing single file through optical system (Figure 1).
Figure 1. Overview of acoustic-assisted hydrodynamic focusing employed in the Attune Flow Cytometers.
Traditional hydrodynamic focusing permits faster flow rates by broadening the width of the sample stream. This can be problematic as a wider core may allow more than one cell to pass through the laser at the same time (Figure 2B). Acoustic focusing does not broaden the core width to accommodate faster sample flow rates (Figure 2A). This type of system helps preserve data quality as single-cell interrogation is more likely to be maintained even at increased sample flow rates (Figure 2C).
Acoustic technology helps the Attune Flow Cytometers achieve sample-throughput rates up to 10 times faster than traditional hydrodynamic focusing systems (Figures 3 and 4) and data acquisition speeds of 35,000 events/sec. Concentrated samples can be diluted and run quickly on the Attune Flow Cytometers, offering your lab rapid high-quality data acquisition capabilities.
We've conducted countless experiments using the Attune Flow Cytometers, many of the same ones scientists are performing in their labs every day. Take a look at the data we've obtained for the following applications:
The Attune Flow Cytometers accommodates a no-wash, no-lyse protocol for whole blood, which dramatically reduces the number of steps you have to perform, the amount of sample that is lost during wash steps, and the amount of handling your cells have to endure. See the no wash, no lyse options sample data
Number of steps | Total sample prep time | |
---|---|---|
Traditional immunophenotyping protocol | 10 | ~1–2 hr |
No wash, no lyse immunophenotyping protocol | 3 | ~15–20 min |
A Journey Through Innovation, The Discovery of Acoustic Focusing and the Attune Flow Cytometer
This mini-webinar discusses how the discovery of sound measurement lead to a series of discoveries culminating in the knowledge needed to use sound in particle focusing.
Flow cytometry data quality depends on collecting information from thousands of individual cells as they pass through the laser interrogation point. The acoustic technology within Attune Flow Cytometers generate ultrasonic waves that act to transport particles to the center of the sample stream. The pre-focused stream is injected into the sheath stream, which applies hydrodynamic pressure to the sample. The combination of these two forces results in a narrow core stream and an increased probability that cells are passing single file through optical system (Figure 1).
Figure 1. Overview of acoustic-assisted hydrodynamic focusing employed in the Attune Flow Cytometers.
Traditional hydrodynamic focusing permits faster flow rates by broadening the width of the sample stream. This can be problematic as a wider core may allow more than one cell to pass through the laser at the same time (Figure 2B). Acoustic focusing does not broaden the core width to accommodate faster sample flow rates (Figure 2A). This type of system helps preserve data quality as single-cell interrogation is more likely to be maintained even at increased sample flow rates (Figure 2C).
Acoustic technology helps the Attune Flow Cytometers achieve sample-throughput rates up to 10 times faster than traditional hydrodynamic focusing systems (Figures 3 and 4) and data acquisition speeds of 35,000 events/sec. Concentrated samples can be diluted and run quickly on the Attune Flow Cytometers, offering your lab rapid high-quality data acquisition capabilities.
We've conducted countless experiments using the Attune Flow Cytometers, many of the same ones scientists are performing in their labs every day. Take a look at the data we've obtained for the following applications:
The Attune Flow Cytometers accommodates a no-wash, no-lyse protocol for whole blood, which dramatically reduces the number of steps you have to perform, the amount of sample that is lost during wash steps, and the amount of handling your cells have to endure. See the no wash, no lyse options sample data
Number of steps | Total sample prep time | |
---|---|---|
Traditional immunophenotyping protocol | 10 | ~1–2 hr |
No wash, no lyse immunophenotyping protocol | 3 | ~15–20 min |
A Journey Through Innovation, The Discovery of Acoustic Focusing and the Attune Flow Cytometer
This mini-webinar discusses how the discovery of sound measurement lead to a series of discoveries culminating in the knowledge needed to use sound in particle focusing.
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