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Use our restriction site finder tool to find the Anza restriction enzymes that best suit your research needs. Search by product name, isoschizomer name, recognition sequence, or SKU number. Search results include restriction enzyme names, cut site, overhang, compatible ends, isoschizomers, and enzyme properties.
Have questions about how restriction enzymes work, where they get their names, or how to troubleshoot common problems? Explore our Restriction Enzyme education resources in the Invitrogen School of Molecular Biology.
B = C or G or T
D = A or G or T
H = A or C or T
K = G or T
M = A or C
N = A or C or G or T
R = A or G
S = C or G
V = A or C or G
W = A or T
Y = C or T
Product name, Cat. No. | Iso- schizomers | Sequence and cut site | Overhang | Anza restriction enzymes resulting in compatible ends | Properties |
---|---|---|---|---|---|
Anza 1 NotI, IVGN0014 | CciNI | GCGGCCGC 5'...G C^G G C C G C...3' 3'...C G C C G G^C G...5' | 5’ protruding ends | Anza 47 Eco52I, Anza 121 Bsp120I | |
Anza 2 NcoI, IVGN0026 | Bsp19I | CCATGG 5'...C^C A T G G...3' 3'...G G T A C^C...5' | 5’ protruding ends | Anza 58 PagI | |
Anza 3 BcuI, IVGN0034 | AhlI, SpeI | ACTAGT 5'...A^C T A G T...3' 3'...T G A T C^A...5' | 5’ protruding ends | Anza 6 NheI, Anza 12 XbaI, Anza 15 XmaJI | |
Anza 4 BpiI, IVGN0044 | BbsI, BstV2I | GAAGAC(2/6) 5'...G A A G A C (N)2^...3' 3'...C T T C T G (N)6^...5' | 5’ protruding ends | ||
Anza 5 BamHI, IVGN0056 | GGATCC 5'...G^G A T C C...3' 3'...C C T A G^G...5' | 5’ protruding ends | Anza 19 BglII, Anza 55 MboI, Anza 73 BclI, Anza 97 Bsp143I | ||
Anza 6 NheI, IVGN0066 | AsuNHI | GCTAGC 5'...G^C T A G C...3' 3'...C G A T C^G...5' | 5’ protruding ends | Anza 3 BcuI, Anza 12 XbaI, Anza 15 XmaJI | |
Anza 7 BshTI, IVGN0074 | AsiGI, CspAI, PinAI, AgeI | ACCGGT 5'...A^C C G G T...3' 3'...T G G C C^A...5' | 5’ protruding ends | Anza 60 Kpn2I, Anza 85 MreI, Anza 90 Eco88I* (*if recognized sequence is 5'-CCCGGG-3') | |
Anza 8 XhoI, IVGN0086 | PaeR7I, Sfr274I, SlaI | CTCGAG 5'...C^T C G A G...3' 3'...G A G C T^C...5' | 5’ protruding ends | Anza 14 SalI, Anza 90 Eco88I* (*if recognized sequence is 5'-CTCGAG-3') | |
Anza 9 NdeI, IVGN0096 | FauNDI | CATATG 5'...C A^T A T G...3' 3'...G T A T^A C...5' | 5’ protruding ends | Anza 64 SaqAI, Anza 76 VspI, Anza 80 FspBI, Anza 122 Csp6I | |
Anza 10 DpnI, IVGN0106 | MalI | Gm6ATC 5'...G m6A^T C...3' 3'...C T^m6A G...5' | blunt | ||
Anza 11 EcoRI, IVGN0116 | GAATTC 5'...G^A A T T C...3' 3'...C T T A A^G...5' | 5’ protruding ends | Anza 31 MunI, Anza 111 XapI | ||
Anza 12 XbaI, IVGN0126 | TCTAGA 5'...T^C T A G A...3' 3'...A G A T C^T...5' | 5’ protruding ends | Anza 3 BcuI, Anza 6 NheI, Anza 15 XmaJI | ||
Anza 13 Esp3I, IVGN0136 | BsmBI | CGTCTC(1/5) 5'...C G T C T C (N)1^...3' 3'...G C A G A G (N)5^...5' | 5’ protruding ends | ||
Anza 14 SalI, IVGN0146 | GTCGAC 5'...G^T C G A C...3' 3'...C A G C T^G...5' | 5’ protruding ends | Anza 8 XhoI, Anza 90 Eco88I* (*if recognized sequence is 5'-CTCGAG-3') | ||
Anza15 XmaJI, IVGN0154 | AspA2I, AvrII, BlnI | CCTAGG 5'...C^C T A G G...3' 3'...G G A T C^C...5' | 5’ protruding ends | Anza 3 BcuI, Anza 6 NheI, Anza 12 XbaI | |
Anza 16 HindIII, IVGN0166 | AAGCTT 5'...A^A G C T T...3' 3'...T T C G A^A...5' | 5’ protruding ends | |||
Anza 17 KpnI, IVGN0176 | GGTACC 5'...G G T A C^C...3' 3'...C^C A T G G...5' | 3’ protruding ends | |||
Anza 18 PacI, IVGN0184 | TTAATTAA 5'…T T A A T^T A A...3' 3'…A A T^T A A T T...5' | 3’ protruding ends | Anza 27 PvuI, Anza 40 SfaAI | ||
Anza 19 BglII, IVGN0196 | AGATCT 5'...A^G A T C T...3' 3'...T C T A G^A...5' | 5’ protruding ends | Anza 5 BamHI, Anza 55 MboI, Anza 73 BclI, Anza 97 Bsp143I | ||
Anza 20 SacI, IVGN0206 | Psp124BI, SstI | GAGCTC 5'...G A G C T^C...3' 3'...C^T C G A G...5' | 3’ protruding ends | Anza 126 Alw21I* (*if recognized sequence is 5'-GAGCTC-3') | |
Anza 21 SgsI, IVGN0214 | AscI, PalAI | GGCGCGCC 5'...G G^C G C G C C...3' 3'...C C G C G C^G G...5' | 5’ protruding ends | Anza 28 MluI, Anza 45 PteI, Anza 95 MauBI | |
Anza 22 SmaI, IVGN0226 | CCCGGG 5'...C C C^G G G...3' 3'...G G G^C C C...5' | blunt | |||
Anza 23 PstI, IVGN0236 | CTGCAG 5'...C T G C A^G...3' 3'...G^A C G T C...5' | 3’ protruding ends | Anza 37 Mph1103I, Anza 126 Alw21I* (*if recognized sequence is 5'-GTGCAC-3') | ||
Anza 24 MssI, IVGN0244 | PmeI | GTTTAAAC 5'...G T T T^A A A C...3' 3'...C A A A^T T T G...5' | blunt | ||
Anza 25 PaeI, IVGN0254 | SphI | GCATGC 5'...G C A T G^C...3' 3'...C^G T A C G...5' | 3’ protruding ends | Anza 56 Hin1II, Anza 98 XceI | |
Anza 26 Eco32I, IVGN0266 | EcoRV | GATATC 5'...G A T^A T C...3' 3'...C T A^T A G...5' | blunt | ||
Anza 27 PvuI, IVGN0274 | Ple19I | CGATCG 5'...C G A T^C G...3' 3'...G C^T A G C...5' | 3’ protruding ends | Anza 18 PacI, Anza 40 SfaAI | |
Anza 28 MluI, IVGN0286 | ACGCGT 5'...A^C G C G T...3' 3'...T G C G C^A...5' | 5’ protruding ends | Anza 21 SgsI, Anza 45 PteI, Anza 95 MauBI | ||
Anza 29 KflI, IVGN0294 | GGGWCCC 5'…G G^G W C C C...3' 3'…C C C W G^G G...5' | 5’ protruding ends | Anza 63 CpoI, Anza 87 Eco47I | ||
Anza 30 Bsu15I, IVGN0306 | Bsa29I, BseCI, BshVI, BspDI, BsuTUI, ClaI | ATCGAT 5'...A T^C G A T...3' 3'...T A G C^T A...5' | 5’ protruding ends | Anza 65 MspI, Anza 88 Bsp119I, Anza 93 HpaII, Anza 103 Psp1406I, Anza 105 Hin1I, Anza 123 Hin6I, Anza 96 XmiI* (*if recognized sequence is 5'-GTCGAC-3') | |
Anza 31 MunI, IVGN0314 | MfeI | CAATTG 5'...C^A A T T G...3' 3'...G T T A A^C...5' | 5’ protruding ends | Anza 11 EcoRI, Anza 111 XapI | |
Anza 32 ApaI, IVGN0326 | GGGCCC 5'...G G G C C^C...3' 3'...C^C C G G G...5' | 3’ protruding ends | |||
Anza 33 LguI, IVGN0334 | BspQI, PciSI, SapI | GCTCTTC(1/4) 5'...G C T C T T C (N)1^...3' 3'...C G A G A A G (N)4^...5' | 5’ protruding ends | ||
Anza 34 Pfl23II, IVGN0344 | BsiWI, PspLI | CGTACG 5'...C^G T A C G...3' 3'...G C A T G^C...5' | 5’ protruding ends | Anza 39 Bsp1407I, Anza 91 Acc65I, Anza 129 BshNI* (*if recognized sequence is 5'-GGTACC-3') | |
Anza 35 Eco47III, IVGN0354 | AfeI, Aor51HI | AGCGCT 5'...A G C^G C T...3' 3'...T C G^C G A...5' | blunt | ||
Anza 36 Eco31I, IVGN0366 | BsaI, Bso31I, BspTNI | GGTCTC(1/5) 5'...G G T C T C (N)1^...3' 3'...C C A G A G (N)5^...5' | 5’ protruding ends | ||
Anza 37 Mph1103I, IVGN0376 | EcoT22I, NsiI, Zsp2I | ATGCAT 5'...A T G C A^T...3' 3'...T^A C G T A...5' | 3’ protruding ends | Anza 23 PstI, Anza 126 Alw21I* (*if recognized sequence is 5'-GTGCAC-3') | |
Anza 38 ScaI, IVGN0386 | BmcAI, ZrmI | AGTACT 5'...A G T^A C T...3' 3'...T C A^T G A...5' | blunt | ||
Anza 39 Bsp1407I, IVGN0396 | BsrGI, BstAUI | TGTACA 5'...T^G T A C A...3' 3'...A C A T G^T...5' | 5’ protruding ends | Anza 34 Pfl23II, Anza 91 Acc65I, Anza 129 BshNI* (*if recognized sequence is 5'-GGTACC-3') | |
Anza 40 SfaAI, IVGN0404 | AsiSI, RgaI, SgfI | GCGATCGC 5'...G C G A T^C G C...3' 3'...C G C^T A G C G...5' | 3’ protruding ends | Anza 18 PacI, Anza 27 PvuI | |
Anza 41 HpyF3I, IVGN0416 | BstDEI, DdeI | CTNAG 5'...C^T N A G...3' 3'...G A N T^C...5' | 5’ protruding ends | ||
Anza 42 RsaI, IVGN0426 | AfaI | GTAC 5'...G T^A C...3' 3'...C A^T G...5' | blunt | ||
Anza 43 Eco105I, IVGN0434 | BstSNI, SnaBI | TACGTA 5'...T A C^G T A...3' 3'...A T G^C A T...5' | blunt | ||
Anza 44 AluI, IVGN0446 | AluBI | AGCT 5'...A G^C T...3' 3'...T C^G A...5' | blunt | ||
Anza 45 PteI, IVGN0454 | BsePI, BssHII, PauI | GCGCGC 5'...G^C G C G C...3' 3'...C G C G C^G...5' | 5’ protruding ends | Anza 21 SgsI, Anza 28 MluI, Anza 95 MauBI | |
Anza 46 AatII, IVGN0464 | GACGTC 5'...G A C G T^C...3' 3'...C^T G C A G...5' | 3’ protruding ends | |||
Anza 47 Eco52I, IVGN0474 | BseX3I, BstZI, EagI, EclXI | CGGCCG 5'...C^G G C C G...3' 3'...G C C G G^C...5' | 5’ protruding ends | Anza 1 NotI, Anza 121 Bsp120I | |
Anza 48 MnlI, IVGN0484 | CCTC(7/6) 5'...C C T C (N)7^...3' 3'...G G A G (N)6^...5' | 3’ protruding ends | |||
Anza 49 SmiI, IVGN0496 | SwaI | ATTTAAAT 5'...A T T T^A A A T...3' 3'...T A A A^T T T A...5' | blunt | ||
Anza 50 KspAI, IVGN0504 | HpaI | GTTAAC 5'...G T T^A A C...3' 3'...C A A^T T G...5' | blunt | ||
Anza 51 BspTI, IVGN0516 | AflII, BfrI, BstAFI, MspCI, Vha464I | CTTAAG 5'...C^T T A A G...3' 3'...G A A T T^C...5' | 5’ protruding ends | ||
Anza 52 PvuII, IVGN0526 | CAGCTG 5'...C A G^C T G...3' 3'...G T C^G A C...5' | blunt | |||
Anza 53 AanI, IVGN0534 | PsiI | TTATAA 5'...T T A^T A A...3' 3'...A A T^A T T...5' | blunt | ||
Anza 54 Eco147I, IVGN0546 | PceI, SseBI, StuI | AGGCCT 5'...A G G^C C T...3' 3'...T C C^G G A...5' | blunt | ||
Anza 55 MboI, IVGN0554 | BfuCI, Bsp143I, BssMI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AI | GATC 5'...^G A T C ...3' 3'... C T A G^...5' | 5’ protruding ends | Anza 5 BamHI, Anza 19 BglII, Anza 73 BclI, Anza 97 Bsp143I | |
Anza 56 Hin1II, IVGN0566 | FaeI, Hsp92II, NlaIII | CATG 5'... C A T G^...3' 3'...^G T A C ...5' | 3’ protruding ends | Anza 25 PaeI, Anza 98 XceI | |
Anza 57 Bpu1102I, IVGN0574 | BlpI, Bsp1720I | GCTNAGC 5'...G C^T N A G C...3' 3'...C G A N T^C G...5' | 5’ protruding ends | ||
Anza 58 PagI, IVGN0584 | CciI, BspHI | TCATGA 5'...T^C A T G A...3' 3'...A G T A C^T...5' | 5’ protruding ends | Anza 2 NcoI | |
Anza 59 HhaI, IVGN0596 | AspLEI, BstHHI, CfoI | GCGC 5'...G C G^C...3' 3'...C^G C G...5' | 3’ protruding ends | ||
Anza 60 Kpn2I, IVGN0606 | AccIII, Aor13HI, BseAI, Bsp13I, BspEI, MroI | TCCGGA 5'...T^C C G G A...3' 3'...A G G C C^T...5' | 5’ protruding ends | Anza 7 BshTI, Anza 85 MreI, Anza 90 Eco88I* (*if recognized sequence is 5'-CCCGGG-3') | |
Anza 61 PfoI, IVGN0614 | TCCNGGA 5'...T^C C N G G A...3' 3'...A G G N C C^T...5' | 5’ protruding ends | |||
Anza 62 MlsI, IVGN0624 | BalI, MluNI, MscI, Msp20I, BspTNI | TGGCCA 5'...T G G^C C A...3' 3'...A C C^G G T...5' | blunt | ||
Anza 63 CpoI, IVGN0634 | CspI, Rsr2I, RsrII | CGGWCCG 5'...C G^G W C C G...3' 3'...G C C W G^G C...5' | 5’ protruding ends | Anza 28 KflI, Anza 87 Eco47I | |
Anza 64 SaqAI, IVGN0644 | MseI, Tru1I, Tru9I | TTAA 5'...T^T A A...3' 3'...A A T^T...5' | 5’ protruding ends | Anza 9 NdeI, Anza 76 VspI, Anza 80 FspBI, Anza 122 Csp6I | |
Anza 65 MspI, IVGN0656 | BsiSI, HapII, Hpall | CCGG 5'...C^C G G...3' 3'...G G C^C...5' | 5’ protruding ends | Anza 30 Bsu15I, Anza 88 Bsp119I, Anza 93 HpaII, Anza 103 Psp1406I, Anza 105 Hin1I, Anza 123 Hin6I, Anza 96 XmiI* (*if recognized sequence is 5'-GTCGAC-3') | |
Anza 66 BstXI, IVGN0666 | CCANNNNNNTGG 5'...C C A N N N N N^N T G G...3' 3'...G G T N^N N N N N A C C...5' | 3’ protruding ends | |||
Anza 67 RruI, IVGN0676 | Bsp68I, BtuMI, NruI | TCGCGA 5'...T C G^C G A...3' 3'...A G C^G C T...5' | blunt | ||
Anza 68 BsuRI, IVGN0686 | BshFI, BsnI, HaeIII, BspANI | GGCC 5'...G G^C C...3' 3'...C C^G G...5' | blunt | ||
Anza 69 BglI, IVGN0696 | GCCNNNNNGGC 5'...G C C N N N N^N G G C...3' 3'...C G G N^N N N N C C G...5' | 3’ protruding ends | |||
Anza 70 NsbI, IVGN0704 | Acc16I, FspI | TGCGCA 5'...T G C^G C A...3' 3'...A C G^C G T...5' | blunt | ||
Anza 71 HinfI, IVGN0716 | GANTC 5'...G^A N T C...3' 3'...C T N A^G...5' | 5’ protruding ends | |||
Anza 72 HincII, IVGN0726 | HindII | GTYRAC 5'...G T Y^R A C...3' 3'...C A R^Y T G...5' | blunt | ||
Anza 73 BclI, IVGN0736 | FbaI, Ksp22I | TGATCA 5'...T^G A T C A...3' 3'...A C T A G^T...5' | 5’ protruding ends | Anza 5 BamHI, Anza 19 BglII, Anza 55 MboI, Anza 97 Bsp143I | |
Anza 74 CsiI, IVGN0744 | ACCWGGT 5'…A^C C W G G T...3' 3'…T G G W C C^A...5' | 5’ protruding ends | |||
Anza 75 Alw44I, IVGN0756 | ApaLI, VneI | GTGCAC 5'...G^T G C A C...3' 3'...C A C G T^G...5' | 5’ protruding ends | Anza 94 BfmI* (*if recognized sequence is 5'-CTGCAG-3') | |
Anza 76 VspI, IVGN0766 | AseI, PshBI | ATTAAT 5'...A T^T A A T...3' 3'...T A A T^T A...5' | 5’ protruding ends | Anza 9 NdeI, Anza 64 SaqAI, Anza 80 FspBI, Anza 122 Csp6I | |
Anza 77 DraI, IVGN0776 | TTTAAA 5'...T T T^A A A...3' 3'...A A A^T T T...5' | blunt | |||
Anza 78 AdeI, IVGN0786 | DraIII | CACNNNGTG 5'...C A C N N N^G T G...3' 3'...G T G^N N N C A C...5' | 3’ protruding ends | ||
Anza 79 PdiI, IVGN0794 | NaeI | GCCGGC 5'...G C C^G G C...3' 3'...C G G^C C G...5' | blunt | ||
Anza 80 FspBI, IVGN0804 | BfaI, MaeI, XspI, SspMl | CTAG 5'...C^T A G...3' 3'...G A T^C...5' | 5’ protruding ends | Anza 9 NdeI, Anza 64 SaqAI, Anza 76 VspI, Anza 122 Csp6I | |
Anza 81 Eco91I, IVGN0816 | BstEII, BstPI, EcoO65I, PspEI | GGTNACC 5'...G^G T N A C C...3' 3'...C C A N T G^G...5' | 5’ protruding ends | ||
Anza 82 Eco72I, IVGN0826 | AcvI, BbrPI, PmaCI, Pm1I, PspCI | CACGTG 5'...C A C^G T G...3' 3'...G T G^C A C...5' | blunt | ||
Anza 83 Eco81I, IVGN0836 | AxyI, Bse21I, Bsu36I | CCTNAGG 5'...C C^T N A G G...3' 3'...G G A N T^C C...5' | 5’ protruding ends | ||
Anza 84 FspAI, IVGN0844 | RTGCGCAY 5'...R T G C^G C A Y...3' 3'...Y A C G^C G T R...5' | blunt | |||
Anza 85 MreI, IVGN0854 | CGCCGGCG 5'...C G^C C G G C G...3' 3'...G C G G C C^G C...5' | 5’ protruding ends | Anza 7 BshTI, Anza 60 Kpn2I, Anza 90 Eco88I* (*if recognized sequence is 5'-CCCGGG-3') | ||
Anza 86 PdmI, IVGN0866 | Asp700I, MroXI, XmnI | GAANNNNTTC 5'...G A A N N^N N T T C...3' 3'...C T T N N^N N A A G...5' | blunt | ||
Anza 87 Eco47I, IVGN0876 | AvaII, Bme18I, SinI, VpaK11BI | GGWCC 5'...G^G W C C...3' 3'...C C W G^G...5' | 5’ protruding ends | Anza 28, KflI, Anza 63 CpoI | |
Anza 88 Bsp119I, IVGN0886 | AsuII, Bpu14I, BspT104I, BstBI, NspV, SfuI | TTCGAA 5'...T T^C G A A...3' 3'...A A G C^T T...5' | 5’ protruding ends | Anza 30 Bsu15I, Anza 65 MspI, Anza 93 HpaII, Anza 103 Psp1406I, Anza 105 Hin1I, Anza 123 Hin6I, Anza 96 XmiI* (*if recognized sequence is 5'-GTCGAC-3') | |
Anza 89 Mva1269I, IVGN0894 | BsmI, PctI | GAATGC(1/-1) 5'...G A A T G C N^...3' 3'...C T T A C^G N ...5' | 3’ protruding ends | ||
Anza 90 Eco88I, IVGN0906 | Ama87I, AvaI, BsiHKCI, BsoBI, BmeT110l | CYCGRG 5'...C^Y C G R G...3' 3'...G R G C Y^C...5' | 5’ protruding ends | If recognized sequence is 5'-CTCGAG-3', then generate compatible ends for Anza 8 XhoI, Anza 14 SalI. If recognized sequence is 5'-CCCGGG-3', then generate compatible ends for Anza 7 BshTI, Anza 60 Kpn2I, Anza 85 MreI | |
Anza 91 Acc65I, IVGN0916 | Asp718I | GGTACC 5'...G^G T A C C...3' 3'...C C A T G^G...5' | 5’ protruding ends | Anza 34 Pfl23II, Anza 39 Bsp1407I, Anza 129 BshNI* (*if recognized sequence is 5'-GGTACC-3') | |
Anza 92 EheI, IVGN0924 | DinI, EgeI, SfoI | GGCGCC 5'...G G C^G C C...3' 3'...C C G^C G G...5' | blunt | ||
Anza 93 HpaII, IVGN0936 | BsiSI, HapII, Mspl | CCGG 5'...C^C G G...3' 3'...G G C^C...5' | 5’ protruding ends | Anza 30 Bsu15I, Anza 65 MspI, Anza 88 Bsp119I, Anza 103 Psp1406I, Anza 105 Hin1I, Anza 123 Hin6I, Anza 96 XmiI* (*if recognized sequence is 5'-GTCGAC-3') | |
Anza 94 BfmI, IVGN0944 | BstSFI, SfcI | CTRYAG 5'...C^T R Y A G...3' 3'...G A Y R T^C...5' | 5’ protruding ends | If recognized sequence is 5'-CTGCAG-3', then generate compatible ends for Anza 75 Alw44I | |
Anza 95 MauBI, IVGN0954 | CGCGCGCG 5'...C G^C G C G C G...3' 3'...G C G C G C^G C...5' | 5’ protruding ends | Anza 21 SgsI, Anza 28 MluI, Anza 45 PteI | ||
Anza 96 XmiI, IVGN0966 | AccI, FblI | GTMKAC 5'...G T^M K A C...3' 3'...C A K M^T G...5' | 5’ protruding ends | If recognized sequence is 5'-GTCGAC-3', then generate compatible ends for Anza 30 Bsu15I, Anza 65 MspI, Anza 88 Bsp119I, Anza 93 HpaII, Anza 103 Psp1406I, Anza 105 Hin1I, Anza 123 Hin6I | |
Anza 97 Bsp143I, IVGN0976 | BfuCI, BssMI, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI | GATC 5'...^G A T C ...3' 3'... C T A G^...5' | 5’ protruding ends | Anza 5 BamHI, Anza 19 BglII, Anza 55 MboI, Anza 73 BclI | |
Anza 98 XceI, IVGN0984 | BstNSI, NspI | RCATGY 5'...R C A T G^Y...3' 3'...Y^G T A C R...5' | 3’ protruding ends | Anza 25 PaeI, Anza 56 Hin1II | |
Anza 99 XagI, IVGN0996 | BstENI, EcoNI | CCTNNNNNAGG 5'...C C T N N^N N N A G G...3' 3'...G G A N N N^N N T C C...5' | 5’ protruding ends | ||
Anza 100 Bsh1236I, IVGN1006 | AccII, BspFNI, BstFNI, BstUI, MvnI | CGCG 5'...C G^C G...3' 3'...G C^G C...5' | blunt | ||
Anza 101 BoxI, IVGN1016 | BstPAI, PshAI | GACNNNNGTC 5'...G A C N N^N N G T C...3' 3'...C T G N N^N N C A G...5' | blunt | ||
Anza 102 CaiI, IVGN1024 | AlwNI, PstNI | CAGNNNCTG 5'...C A G N N N^C T G...3' 3'...G T C^N N N G A C...5' | 3’ protruding ends | ||
Anza 103 Psp1406I, IVGN1034 | AclI | AACGTT 5'...A A^C G T T...3' 3'...T T G C^A A...5' | 5’ protruding ends | Anza 30 Bsu15I, Anza 65 MspI, Anza 88 Bsp119I, Anza 93 HpaII, Anza 105 Hin1I, Anza 123 Hin6I, Anza 96 XmiI* (*if recognized sequence is 5'-GTCGAC-3') | |
Anza 104 MboII, IVGN1044 | GAAGA(8/7) 5'...G A A G A (N)8^...3' 3'...C T T C T (N)7^...5' | 3’ protruding ends | |||
Anza 105 Hin1I, IVGN1056 | AcyI, BsaHI, BssNI, BstACI, Hsp92I | GRCGYC 5'...G R^C G Y C...3' 3'...C Y G C^R G...5' | 5’ protruding ends | Anza 30 Bsu15I, Anza 65 MspI, Anza 88 Bsp119I, Anza 93 HpaII, Anza 103 Psp1406I, Anza 123 Hin6I, Anza 96 XmiI* (*if recognized sequence is 5'-GTCGAC-3') | |
Anza 106 Van91I, IVGN1066 | AccB7I, PflMI | CCANNNNNTGG 5'...C C A N N N N^N T G G...3' 3'...G G T N^N N N N A C C...5' | 3’ protruding ends | ||
Anza 107 BspLI, IVGN1074 | BmiI, NlaIV, PspN4I | GGNNCC 5'...G G N^N C C...3' 3'...C C N^N G G...5' | blunt | ||
Anza 108 SatI, IVGN1084 | Fnu4HI, Fsp4HI | GCNGC 5'...G C^N G C...3' 3'...C G N^C G...5' | 5’ protruding ends | ||
Anza 109 Alw26I, IVGN1096 | BcoDI, BsmAI, BstMAI | GTCTC(1/5) 5'...G T C T C (N)1^...3' 3'...C A G A G (N)5^...5' | 5’ protruding ends | ||
Anza 111 XapI, IVGN1116 | AcsI, ApoI | RAATTY 5'...R^A A T T Y...3' 3'...Y T T A A^R...5' | 5’ protruding ends | Anza 11 EcoRI, Anza 31 MunI | |
Anza 112 BseGI, IVGN1126 | BstF5I, BtsCI | GGATG(2/0) 5'...G G A T G N N^...3' 3'...C C T A C^N N ...5' | 3’ protruding ends | ||
Anza 113 BcnI, IVGN1136 | AsuC2I, BpuMI, NciI | CCSGG 5'...C C^S G G...3' 3'...G G S^C C...5' | 5’ protruding ends | ||
Anza 114 Hpy8I, IVGN1146 | Hpy166II | GTNNAC 5'...G T N^N A C...3' 3'...C A N^N T G...5' | blunt | ||
Anza 115 MbiI, IVGN1156 | AccBSI, BsrBI | CCGCTC (-3/-3) 5'...C C G^C T C...3' 3'...G G C^G A G...5' | blunt | ||
Anza 116 Cfr13I, IVGN1166 | AspS9I, BmgT120I, PspPI, Sau96I | GGNCC 5'...G^G N C C...3' 3'...C C N G^G...5' | 5’ protruding ends | ||
Anza 117 EcoO109I, IVGN1176 | RGGNCCY 5'...R G^G N C C Y...3' 3'...Y C C N G^G R...5' | 5’ protruding ends | |||
Anza 118 BseDI, IVGN1186 | BsaJI, BssECI | CCNNGG 5'...C^C N N G G...3' 3'...G G N N C^C...5' | 5’ protruding ends | ||
Anza 119 BmsI, IVGN1194 | LweI, SfaNI | GCATC (5/9) 5'…G C A T C (N)5^...3' 3'…C G T A G (N)9^...5' | 5’ protruding ends | ||
Anza 120 NmuCI, IVGN1204 | TseFI, Tsp45I | GTSAC 5'...^G T S A C ...3' 3'... C A S T G^...5' | 5’ protruding ends | ||
Anza 121 Bsp120I, IVGN1216 | PspOMI | GGGCCC 5'...G^G G C C C...3' 3'...C C C G G^G...5' | 5’ protruding ends | Anza 1 NotI, Anza 47 Eco52I | |
Anza 122 Csp6I, IVGN1226 | CviQI, RsaNI | GTAC 5'...G^T A C...3' 3'...C A T^G...5' | 5’ protruding ends | Anza 9 NdeI, Anza 64 SaqAI, Anza 76 VspI, Anza 80 FspBI | |
Anza 123 Hin6I, IVGN1236 | HinP1I, HspAI | GCGC 5'...G^C G C...3' 3'...C G C^G...5' | 5’ protruding ends | Anza 30 Bsu15I, Anza 65 MspI, Anza 88 Bsp119I, Anza 93 HpaII, Anza 103 Psp1406I, Anza 105 Hin1I, Anza 96 XmiI* (*if recognized sequence is 5'-GTCGAC-3') | |
Anza 124 PfeI, IVGN1244 | TfiI | GAWTC 5'...G^A W T C...3' 3'...C T W A^G...5' | 5’ protruding ends | ||
Anza 125 HpyF10VI, IVGN1254 | BstMWI, MwoI | GCNNNNNNNGC 5'...G C N N N N N^N N G C...3' 3'...C G N N^N N N N N C G...5' | 3’ protruding ends | ||
Anza 126 Alw21I, IVGN1266 | Bbv12I, BsiHKAI | GWGCWC 5'...G W G C W^C...3' 3'...C^W C G W G...5' | 3’ protruding ends | If recognized sequence is 5'-GAGCTC-3', then generate compatible ends for Anza 20 SacI. If recognized sequence is 5'-GTGCAC-3', then generate compatible ends for Anza 23 PstI, Anza 37 Mph1103I | |
Anza 127 RseI, IVGN1274 | MslI, SmiMI | CAYNNNNRTG 5'...C A Y N N^N N R T G...3' 3'...G T R N N^N N Y A C...5' | blunt | ||
Anza 128 PspFI, IVGN1284 | GsaI | CCCAGC(-1/-5) 5'…C C C A G^C...3' 3'…G^G G T C G...5' | 3’ protruding ends | ||
Anza 129 BshNI, IVGN1294 | AccB1I, BanI, BspT107I | GGYRCC 5'...G^G Y R C C...3' 3'...C C R Y G^G...5' | 5’ protruding ends | If recognized sequence is 5'-GGTACC-3', then generate compatible ends for Anza 34 Pfl23II, Anza 39 Bsp1407I, Anza 91 Acc65I |
For Research Use Only. Not for use in diagnostic procedures.