Absolute Q Custom Digital PCR Assays | Thermo Fisher Scientific

Custom digital PCR made powerfully simple

You may need to be an expert in your field, but you shouldn't need to be an expert in dPCR assay design. That's why we've programmed over 40 years of Applied Biosystems assay design experience into the proprietary algorithms powering our new Absolute Q dPCR Custom Assay Design Tool. With this tool, you have years of design expertise at your fingertips to help generate custom probe-based digital PCR (dPCR) assays for research with confidence and ease.

No assay design experience required

If you know your target, it’s as easy as 1, 2, 3:

1. Choose your application

2. Input target sequences

3. Review design and checkout

Ready to get started with your dPCR experiment, but need support designing custom assays for your targets of interest? With our custom dPCR assay tool you don’t have to be an expert. Let our decades of expertise in PCR assay design ease the process so that you can focus on what matters.

Go to custom assay tool

Benefits of the Absolute Q dPCR custom assay design tool

Simple—enter your sequences and receive ready-to-order assay designs in just a few steps
Robust—built using Applied Biosystems trusted assay design algorithms
High quality—designed using gold-standard TaqMan chemistry and minor groove binder (MGB) moiety for better sequence discrimination than non-MGB probes

What sets Absolute Q dPCR assays apart?

Absolute Q dPCR assays are based on the same gold-standard TaqMan chemistry used in real-time PCR and cited in more than 200K research publications. The assays feature a proprietary probe with a minor groove binder (MGB) moiety at the 3’ end that increases the melting temperature (T_m) of the probe. This means MGB probes can be significantly shorter than traditional approaches, providing high-resolution sequence discrimination and flexibility to accommodate more targets in a single reaction.

Looking for custom multiplex dPCR assay design?

Contact us and let your local representative help find the best solution for your custom multiplex dPCR assay design needs.

For Research Use Only. Not for use in diagnostic procedures.

Style Sheet for Global Design System