Protein Gels

Exceptional protein gel electrophoresis is the cornerstone of a successful western blotting experiment. Invitrogen precast protein gels are offered in four different chemistries, allowing you to tailor the separation to your target protein's molecular weight. This helps obtain sharp, well-resolved bands, and results in efficient transfer and improved detection of your protein.

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WedgeWell format wells are wedge-shaped wells that increase the sample loading capacity, allowing up to 100 µL of sample to be loaded in the well. The WedgeWell design was introduced in 2012 to increase the sample loading capacity of Invitrogen precast mini gels.

More than a thousand peer-reviewed articles demonstrate how WedgeWell format gels help produce publication-quality results and enable the study of low abundance proteins, reducing the need to enrich dilute samples before loading them onto the gel.

Midi gels with WedgeWell format wells allow the separation of up to 26 protein samples in the same run without compromising on sample loading volume.

Key benefits of WedgeWell format gels:

• Results you can trust—rigorously tested to help ensure reliable, reproducible performance, lot after lot
• Easy sample loading—larger well openings make loading a breeze even with standard pipette tips, and reduce sample spill-over and cross well contamination
• Increased sample loading capacity—easy detection of dilute samples and low abundance proteins
• Reduced cost—lower cost per sample and reduced running buffer required per sample (7.3 mL of running buffer saved per sample, 140 mL saved for 20 samples)*
• Faster run times—up to 10 min faster than traditional gels

Available in three different gel chemistries:

• Bis- Tris
• Tris-Glycine
• Tris-Acetate

All Invitrogen protein gels meet the highest standards of performance, reliability, and consistency.

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*Based on comparison between our most popular mini and midi well format options (12 and 20 well, respectively)

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For separation of proteins under nondenaturing conditions or native page, three gel systems are recommended- NativePAGE Bis-Tris, Novex Tris-Glycine and NuPAGE Tris-Acetate. NativePAGE Bis-Tris gels, are based on the blue native polyacrylamide gel electrophoresis (BN-PAGE) which helps overcome the limitations of traditional native electrophoresis by providing a near-neutral operating pH and detergent compatibility. Both Novex Tris-Glycine and NuPAGE Tris-Acetate gels can be run under native and non-reducing conditions for separation of proteins by net charge, size and shape.

For separation of proteins by isoelectroctric point (pI), use Novex IEF gels. Novex IEF gels effectively create a pH gradient to separate proteins according to their unique pI.

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1. Libardo, M.D.J.; Durr, E.; Hernandez, L.D. A Robust Protocol to Isolate Outer Membrane Vesicles from Nontypeable Haemophilus influenzae.Methods Protoc.2023,6, 42. https://doi.org/10.3390/mps6020042
2. Horita, H., Law, A., Middleton, K. Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins. J. Vis. Exp. (131), e56912, doi:10.3791/56912 (2018).