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Why choose a Vanquish Online 2D-LC System?
- Develop deeper knowledge of your sample using automated in-depth characterization steps
- Gain more confidence by confirming peak identification, purity and identity of complex samples
- Flexibility to switch between two independent UHPLC systems or one 2D-LC system with the Thermo Scientific Vanquish Simple Switch System – Download technical note for details
What is online 2D-LC?
Two-dimensional liquid chromatography is a multidimensional separation technique to achieve full characterization of substances or peak purity confirmation. One compound, or one fraction, is analyzed by two different separation modes to add additional selectivity to resolve difficult analytes or deplete interfering matrix.
There are different types of 2D-LC techniques differing in automation degree, as well as number and storage of fractions. Unlike offline 2D-LC where fractions are collected and then re-injected onto a second column, online 2D-LC involves transferring the fractions of the first-dimension separation to a second-dimension column in a single flow path inside the system.
Configuration options
| Vanquish Online SPE HPLC and UHPLC | Vanquish Loop Heart-Cut 2D-LC | Vanquish Trap Heart-Cut 2D-LC | Vanquish Simple Switch 2D-LC | |
|---|---|---|---|---|
| Automated online sample cleanup | Yes | |||
| Analyte enrichment | Yes | Yes | Yes | |
| Ability to run two complementary column chemistries | Yes | Yes | Yes | |
| Ability to optimize mass spectrometry compatibility of buffer-based methods by desalting | Yes | Yes | Yes | |
| Flexibility to run system as two independent 1D-LC systems | Yes |
Features automated online sample preparation to save time and solvents.
Thermo Scientific Vanquish Online SPE HPLC and UHPLC Systems automate analyte pre-concentration and sample clean-up using a repeatable workflow. The process removes the sample matrix via a bidirectional trap column before the sample is eluted for separation on the analytical column to save solvent and time compared to offline sample preparation steps.
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The figure illustrates how the six-port valve is used to switch the trap column between sample loading and elution.
Related literature
Straightforward peak purity confirmation by LC-LC
The Thermo Scientific Vanquish Loop Heart-Cut 2D-LC System can reveal hidden impurities that co-elute with the analytes of interest by using two complementary column chemistries to gain more separation power. This system enables you to collect one or multiple fractions of the first-dimension separation in one or multiple loops and transfer the cut-out fraction(s) in an automated workflow to a second-dimension separation.
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An illustration showing the plumbing of the Vanquish Online 2D-LC system for multiple heart-cutting, enabling storage of the cut-out fractions in multiple loops before performing a complementary analysis on a second-dimension (2D) column.
Related Literature
Enhance freedom and performance of 2D-LC method combinations
The Thermo Scientific Vanquish Trap Heart-Cut 2D-LC System facilitates a wide range of 1D and 2D separation combinations and simplifies multi-dimensional LC method development. It can reveal hidden impurities that co-elute with the analytes of interest by applying two complementary column chemistries to gain more separation power. This system enables you to collect a single fraction of the first-dimension separation onto a trap column to pre-concentrate low-abundant analytes and addresses solvent incompatibility issues before the fraction is eluted onto a second dimension (2D) column to resolve difficult or co-eluting peaks.
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The figure illustrates the two six-port valves in combination with the 2D pump and the T-piece are used to reduce the solvent strength and to load the cut-out 1D fraction onto the trap column to pre-concentrate the sample before being loaded on the second dimension (2D) column.
Related Literature
Seamlessly switch between confidence with 2D-LC and productivity with Dual LC
The all-in-one system Thermo Scientific Vanquish Simple Switch 2D-LC System allows you to seamlessly switch between a trap heart-cut 2D-LC system and a dual LC system. This innovative system uses the novel Thermo Scientific Vanquish Dual Split Sampler to enable users to switch between system capabilities without re-plumbing. Simply decide which task needs to be performed and this system supplies the confidence and productivity you need.
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An illustration of how the Dual Split Sampler and three six-port valves can be used to run the system for 2D-LC analysis with two columns with a trap column in series, or as a dual LC system with two columns in parallel without replumbing the system. The flow scheme depicts the Simple Switch 2D-LC system as a dual LC system running two 1D-LC separations simultaneously to two detectors.
Related Literature
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Multiple options for increased confidence with Thermo Scientific Vanquish Online 2D-LC systems.
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