Graphic showing activated T cell with multicolor highlights in background

Spectral flow cytometry analysis allows researchers and scientists the ability to investigate an increasing number of molecules of interest. Learn how spectral flow cytometry technologies provide resolution to your complex research and experimental needs.

Featured videos

Why are researchers excited about spectral flow cytometry?

Video on how spectral flow cytometry enables researchers to get more information about their cells in a single flow cytometry experiment

Imagine spectral possibilities

Video expresses how much more information you can get from a cell using high parameter/spectral flow cytometry

 

Expand panels with higher multidimensionality

Achieve high dimensional data with spectral flow cytometry by combining smaller panels into a single larger panel

 

Benefits of spectral flow cytometry

Improvement in flow cytometry instrumentation that provides greater flexibility, efficiency, and capabilities.  Spectral flow cytometry enables you to get high throughput and high dimensional data, even from precious samples of limited size.

Using the panel builder tool to create a panel to run on a spectral instrument

The Invitrogen Flow Cytometry Panel Builder tool by Thermo Fisher Scientific can be used to build panels for spectral flow cytometers, including those from other suppliers.

 
 

Controls and considerations for adapting a conventional panel to spectral flow cytometry

Learn about the controls and considerations needed for adapting a conventional flow cytometry panel to spectral flow cytometry experiment. Spectral flow cytometry makes it possible to add more fluorochromes to your panel and achieve high parametric and multi-dimensional data sets.

Getting to the spectral mindset from traditional conventional flow - the great rethink

How should one think about their experiments while moving to spectral flow cytometry? Watch to learn more!

 

 
 

Spectral Overlap

How does one think about Sepctral Overlap? What does it mean in the realm of Spectral Flow Cytometry? Watch to learn more!

Spectral flow cytometry educational content

Click on each of the robust education articles below to learn more about spectral flow cytometry. Topics include the workflow differences between conventional and spectral methods, panel design, control and sample preparation, panel evaluation, and data analysis. If you are considering transitioning from conventional to spectral flow cytometry this is a great place to start.

Resources

Flow cytometry
 

We are committed to accelerating your science by providing a comprehensive suite of solutions for the analysis of cells and their function by delivering you our flagship flow cytometry products designed to deliver high-performance results and save you time.

Explore flow cytometry

Flow Cytometry Protocols Handbook
 

Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping and data analysis strategies.

Download now

Fluorophore and reagent selection guide for flow cytometry

A handy reference poster featuring the broad range of our dyes and labeling reagents available.

Key features include:

  • Recommended dyes for your flow cytometer based on lasers and detection channels settings
  • Dye recommendations for UV, Violet, Blue, Green, Yellow, Red, and Near IR lasers
  • Check out our specials—Brilliant Ultra Violet™, Brilliant Violet™, and NovaFluor dyes; more dyes for spectral needs

    • Request a guide

    Stylesheet for Classic Wide Template adjustments

    Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.

    Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

    For Research Use Only. Not for use in diagnostic procedures.