Technical Resources

B-27 Serum-Free Supplement (50X) liquid

B-27 Serum-Free Supplement is used for growth and long-term viability of hippocampal neurons.

This supplement is to be used with a combination of basal medium such as Neurobasal Medium. Below is the composition list. The concentrations are proprietary.

ComponentsMolecular WeightConcentration (mg/L)mM
Vitamins
Biotinconfidentialn/a
DL Alpha Tocopherol Acetateconfidentialn/a
DL Alpha-Tocopherolconfidentialn/a
Vitamin A (acetate)confidentialn/a
Proteins
BSA, fatty acid free Fraction Vconfidentialn/a
Catalaseconfidentialn/a
Human Recombinant Insulinconfidentialn/a
Human Transferrinconfidentialn/a
Superoxide Dismutaseconfidentialn/a
Other Components
Corticosteroneconfidentialn/a
D-Galactoseconfidentialn/a
Ethanolamine HClconfidentialn/a
Glutathione (reduced)confidentialn/a
L-Carnitine HClconfidentialn/a
Linoleic Acidconfidentialn/a
Linolenic Acidconfidentialn/a
Progesteroneconfidentialn/a
Putrescine 2HClconfidentialn/a
Sodium Seleniteconfidentialn/a
T3 (triodo-I-thyronine)confidentialn/a

Reference:

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