Immunocytochemistry image of A21975, peroxisomal multifunctional enzyme 2 (MFE2) monoclonal antibody.

Human HDFn cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody at 5 µg/mL overnight at 4°C. Secondary antibody: (red) Alexa Fluor® 594 Goat Anti-Mouse IgG (H+L) at a 1/1000 dilution for 1 hr. 10% goat serum was used as the blocking agent for all blocking steps. Target protein locates mainly in peroxisome.

Human HDFn cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody at 5 µg/mL overnight at 4°C. Secondary antibody: (red) Alexa Fluor® 594 Goat Anti-Mouse IgG (H+L) at a 1/1000 dilution for 1 hr. 10% goat serum was used as the blocking agent for all blocking steps. Target protein locates mainly in peroxisome.

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