False-color image of free Ca2+ concentration in a Purkinje neuron from embryonic mouse cerebellum.

False-color image of free Ca2+ concentration in a Purkinje neuron from embryonic mouse cerebellum. Neurons were grown in dispersed tissue culture for 12 days, loaded with the pentapotassium salt of fura-2 (Cat. no. F1200) using a microelectrode and then challenged with trans-ACPD, an agonist of metabotropic glutamate receptors, in the absence of extracellular Ca2+. The composite image, which represents the ratio of images obtained with excitation at 340 nm and 380 nm, reveals the mobilization of internal Ca2+ stores without contribution from Ca2+ influx. The image was contributed by D.J. Linden, Johns Hopkins University, and M. Smeyne and J.A. Connor, Roche Institute of Molecular Biology.

False-color image of free Ca2+ concentration in a Purkinje neuron from embryonic mouse cerebellum. Neurons were grown in dispersed tissue culture for 12 days, loaded with the pentapotassium salt of fura-2 (Cat. no. F1200) using a microelectrode and then challenged with trans-ACPD, an agonist of metabotropic glutamate receptors, in the absence of extracellular Ca2+. The composite image, which represents the ratio of images obtained with excitation at 340 nm and 380 nm, reveals the mobilization of internal Ca2+ stores without contribution from Ca2+ influx. The image was contributed by D.J. Linden, Johns Hopkins University, and M. Smeyne and J.A. Connor, Roche Institute of Molecular Biology.

Related Products

Related Images

CD335 (NKp46) Antibody (63335182) in RE Go ›

CD223 (LAG-3) Antibody (56223942) in TM Go ›

REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›

Pseudocolored images of changes in intracellular free Ca2+ in AtT-20/D16v-F2 cells, monitored at 9 sec intervals with fluo-4, AM (F14201, F14202, F14217, F23917). Go ›

Developing Drosophila embryo Go ›

Cytoskeleton of a mixed population of granule neurons and glial cells Go ›

Live cell imaging with CellLight™ reagents. Go ›

Live cells transduced with Organelle Lights™ or Cellular Lights™ reagents. Go ›