Mitochondrial membranes. JC-1.

Potential-dependent staining of mitochondria in CCL64 fibroblasts by JC-1 (T3168). The mitochondria were visualized by epifluorescence microscopy using a 520 nm longpass optical filter. Regions of high mitochondrial polarization are indicated by red fluorescence due to J-aggregate formation by the concentrated dye. Depolarized regions are indicated by the green fluorescence of the JC-1 monomers. The image was contributed by Lan Bo Chen, Dana Farber Cancer Institute, Harvard Medical School.

Potential-dependent staining of mitochondria in CCL64 fibroblasts by JC-1 (T3168). The mitochondria were visualized by epifluorescence microscopy using a 520 nm longpass optical filter. Regions of high mitochondrial polarization are indicated by red fluorescence due to J-aggregate formation by the concentrated dye. Depolarized regions are indicated by the green fluorescence of the JC-1 monomers. The image was contributed by Lan Bo Chen, Dana Farber Cancer Institute, Harvard Medical School.

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