Adult rat cortical astrocyte. Rhod-2 AM and MitoFluor™ Green stain.

Colocalization of fluorescent staining by rhod-2 AM (Cat. no. R1244, R1245MP; upper panel) and mitochondrion-selective MitoFluor™ Green stain (Cat. no. M7502; lower panel) in an adult rat cortical astrocyte. Cells were simultaneously loaded with rhod-2 AM (4.5 µM) and the MitoFluor™ Green stain (20 nM) for 30 minutes at 22°C. Confocal laser-scanning microscopy using 488 nm excitation and spectrally resolved detection, at 505–530 nm for MitoFluor™ Green stain and >=585 nm for rhod-2, shows almost identical staining distribution. The images were contributed by Michael Duchen, University College, London. Reproduced with permission from J Cell Biol 145, 795 (1999).

Colocalization of fluorescent staining by rhod-2 AM (Cat. no. R1244, R1245MP; upper panel) and mitochondrion-selective MitoFluor™ Green stain (Cat. no. M7502; lower panel) in an adult rat cortical astrocyte. Cells were simultaneously loaded with rhod-2 AM (4.5 µM) and the MitoFluor™ Green stain (20 nM) for 30 minutes at 22°C. Confocal laser-scanning microscopy using 488 nm excitation and spectrally resolved detection, at 505–530 nm for MitoFluor™ Green stain and >=585 nm for rhod-2, shows almost identical staining distribution. The images were contributed by Michael Duchen, University College, London. Reproduced with permission from J Cell Biol 145, 795 (1999).

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