Bacillus subtilis sporulation.

Correlated fluorescence imaging of membrane migration, protein translocation, and chromosome localization during Bacillus subtilis sporulation. Membranes were stained with red-fluorescent FM® 4-64 (Cat. No. T3166, T13320). Chromosomes were localized with the blue-fluorescent nuclear counterstain, DAPI (Cat. No. D1306, D3571, D21490). The small, green-fluorescent patches (top row) indicate the localization of a GFP fusion to SpoIIIE, a protein essential for both initial membrane fusion and forespore engulfment. Progression of the engulfment is shown from left to right. Green fluorescence in the middle and bottom rows demonstrates fully engulfed sporangia stained with MitoTracker® Green FM (Cat. No. M7514). Full details of the experimental methods and interpretation are published in Proc Natl Acad Sci U S A 96, 14553 (1999). Image contributed by Kit Pogliano and Marc Sharp, University of California at San Diego. Reproduced from the 7 December 1999 issue of Proc Natl Acad Sci U S A, with permission.

Correlated fluorescence imaging of membrane migration, protein translocation, and chromosome localization during <i>Bacillus subtilis</i> sporulation. Membranes were stained with red-fluorescent FM® 4-64 (Cat. No. T3166, T13320). Chromosomes were localized with the blue-fluorescent nuclear counterstain, DAPI (Cat. No. D1306, D3571, D21490). The small, green-fluorescent patches (top row) indicate the localization of a GFP fusion to SpoIIIE, a protein essential for both initial membrane fusion and forespore engulfment. Progression of the engulfment is shown from left to right. Green fluorescence in the middle and bottom rows demonstrates fully engulfed sporangia stained with MitoTracker® Green FM (Cat. No. M7514). Full details of the experimental methods and interpretation are published in Proc Natl Acad Sci U S A 96, 14553 (1999). Image contributed by Kit Pogliano and Marc Sharp, University of California at San Diego. Reproduced from the 7 December 1999 issue of <i>Proc Natl Acad Sci U S A</i>, with permission.

Related Products

Related Images

1% Agarose gel containing 16S and 23S ribosomal RNA (rRNA). SYBR® Green II RNA gel stain. Go ›

Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›

Cyanobacteria. BODIPY® FL, DAPI and CellTracker™ Green BODIPY®. Go ›

Bifidobacterium sp. bacteria stained with the ViaGram™ Red+ Bacterial Gram Stain and Viability Kit. Go ›