Lentiviral Vector Production Workflow

Lentiviral vector manufacturing solutions

Lentiviral vectors are a widely used modality for gene modification in cell therapy manufacturing processes. The innovative lentivirus production solutions from Thermo Fisher Scientific are designed to accelerate and streamline the development of gene-modified cell therapies and gene therapies.

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• DNA vectors
• Cell culture and transfection
• Single-use fermentors
• Gibco peptones
• Affinity, ion exchange and hydrophobic interaction chromatography resins
• Residual DNA quantitation

• Invitrogen GeneArt gene synthesis

Lentiviral production systems

• CTS LV-MAX Production System
• CTS Viral Production Cells
• Bioreactor systems

Bioprocessing liquids and cell culture media preparation 

• HEK Media Panel
• Gibco peptones
• Bioprocessing liquids

Purification

To scale your research, we have developed chromatography resins for lentiviral vectors along with single-use solutions for robust vector processing.

• POROS affinity and anion exchange chromatography

Mixers, buffers and single-use solutions

• Single-use mixers
• Custom buffers and bags
• Fluid transfer solutions

Analytics and characterization development

To help ensure regulatory approval of the final lentiviral vector product, QC testing must be implemented. We offer rapid molecular methods for quantification, contaminant and impurity testing across the workflow.

• Mycoplasma detection
• Residual host cell DNA quantitation
• Residual plasmid DNA quantitation
• ViralSEQ Lentivirus quantitation

Fill and finish development

Once the final product has been purified, passed the necessary quality and release testing criteria for clinical use, it is ready for formulation, final fill and finish.

• Critical environment containers
• Fluid and transfer solutions
• Sterile drug dosage forms (services)

CRO and CDMO Services

Our cell and gene therapy CRO solutionssupport customers through the unique demands that cell and gene therapy clinical trials call for. The extensive breadth and depth of therapeutic and functional expertise have guided a diverse range of cell and gene therapy assets through the development pipeline

Our Viral Vector Contract Manufacturing Services support clients from drug development to full-scale manufacturing. In addition to product release, stability testing, process and analytical qualification, and custom cell line engineering, we provide global quality compliance and regulatory support.