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Overview
(U)HPLC columns optimized for your analytical performance
Solve your separation challenges with our three premier brands of HPLC and UHPLC columns for small molecules. Our popular product lines of Hypersil GOLD, Accucore, and Acclaim columns are designed for a wide range of applications and offer the following features:
- High-resolution, high-pressure HPLC and UHPLC columns
- Built on a 40-year legacy of innovation
- Unmatched variety of stationary phase chemistries, particle sizes, and column formats
- Long column lifetime with reproducible results
- The broadest assortment of solid core columns on the market
From the most commonly-used C18 phases, to innovative mixed-mode chemistries, we have the right column to fulfill your chromatography needs
Meet our (U)HPLC column families
Hypersil GOLD columns
Fully porous for simple samples
Fully porous, ultrapure silica particle columns- Built on 40 years of experience in column development and manufacturing
- Excellent resolution, efficiency, and sensitivity for routine, high-quality separations
- Fully scalable from UHPLC to prep
Accucore columns
Solid core for high resolution
- Solid core silica technology columns
- UHPLC efficiency on any instrument
- Rapid, high-resolution separation of complex samples and limited sample volumes
- Increased sensitivity and peak capacity using standard HPLC
Acclaim columns
Fully porous for complicated samples
- Fully porous, highly retentive silica particle columns
- Ideal for complex mixtures that require higher resolution
- Wide variety of column chemistries with various selectivities, including mixed-mode and HILIC
Understanding Diversity in HPLC Chromatography Chemistries
Learn how to choose your HPLC chemistry
- What’s the best column to maximize your productivity?
- Understanding the wide spectrum of selectivities
- Superficially porous vs. fully porous packing material
- Unique phases for challenging sample mixtures
Hypersil GOLD HPLC and UHPLC columns
Your first choice of (U)HPLC columns for general analyses
- The go-to, easy-to-use HPLC columns for a variety of chromatographic separations
- Built on 40 years of experience in column development and manufacturing
- Available in a complete range of particle sizes, allowing for full scalability from UHPLC to prep – perform R&D on a high-resolution 1.9 µm column and transfer easily to QC and production
- Outstanding peak shape for reversed phase, ion-exchange, HILIC, or normal phase chromatography
- Excellent resolution, efficiency, and sensitivity
Explore our complete range of Hypersil GOLD columns to start your chromatography analyses
| Hydrophobic Column Phases | Reversed phase chemistry for separation via hydrophobic interactions. Stationary phases with longer carbon chains provide longer retention times and greater hydrophobicity.
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| Polar-Modified C18 Column Phases | Good for the separation of acidic and basic compounds via reversed phase chromatography using an aqueous mobile phase.
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| Phenyl-Based Column Phases | Orthogonal selectivity to traditional hydrophobic phases that utilizes aromatic interactions to achieve challenging separations of aromatic and polar compounds. Phenyl, PFP |
| Ion-Exchange Column Phases | Ionic and polar compounds are retained via hydrophilic and ionic interactions with a charged stationary phase. Retained compounds can be eluted by changing the solvent conditions of the column.
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| Hydrophilic Columns | Ideal for retaining and separating polar compounds based on polarity differences and affinity for a polar stationary phase. HILIC, Silica |
Learn more about Hypersil GOLD columns
Accucore HPLC and UHPLC columns
Solid core silica particles for greater speed and resolution
- High-resolution separation on rugged, solid core, superficially porous particles
- Fast and easy analyses with a wide selection of chemistries
- Increased sensitivity on a standard HPLC system
- Compatible with almost any instrument
Discover high-throughput columns for rapid (U)HPLC separations
| Hydrophobic Column Phases | Reversed phase chemistry for separation via hydrophobic interactions. Stationary phases with longer carbon chains provide longer retention times and greater hydrophobicity. C18, C8, C4 |
| Specialty Hydrophobic Column Phases | Alternative selectivity to C18 for hydrophobic retention and the geometric separation of structural isomers. RP-MS, C30 |
| Phenyl-Based Column Phases | Orthogonal selectivity to traditional hydrophobic phases that utilizes aromatic interactions to achieve challenging separations of aromatic and polar compounds. Biphenyl, Phenyl-Hexyl, PFP |
| Polar-Modified C18 Column Phases | Good for the separation of acidic and basic compounds via reversed phase chromatography using an aqueous mobile phase Polar Premium, aQ |
| HILIC Column Phases | Ideal for retaining and separating polar compounds based on polarity differences and affinity for a polar stationary phase. HILIC, Amide HILIC |
Phase Name | 1.5 μm | 2.6 μm | 4 μm | Pore Size (Å) | Surface Area (m²/g) | Carbon Load (%) | USP |
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| — | 80 | 130 | 9 | L1 | |
| — | — | 80 | 130 | 6.5 | L1 | |
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| 80 | 130 | 7 | L1 | |
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| — | 80 | 130 | 9 | L7 | |
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| 80 | 130 | 4 | L7 | |
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| — | 150 | 80 | 2 | L26 | |
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| — | 80 | 130 | 9 | L1 | |
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| — | 80 | 130 | 8 | L60 | |
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| — | 80 | 130 | 7 | L1 | |
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| — | 80 | 130 | 5 | L62 | |
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| — | 80 | 130 | 6 | L11 | |
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| — | 80 | 130 | 5 | L11 | |
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| — | 80 | 130 | 5 | L43 | |
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| — | 80 | 130 | 6 | – | |
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| — | 80 | 130 | – | L3 | |
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| — | 150 | 80 | – | – | |
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| — | 80 | 130 | – | – |
Learn more about Accucore columns
Acclaim HPLC and UHPLC columns
Our broadest stationary phase variety for high-efficiency separations
- The go-to column for complex mixtures that require greater resolution
- More phases, more choices tailored to your analysis needs
- Particles are fully porous with a high surface area, ensuring high sample retention
- Designed for complicated samples
- Separates and resolves compounds when other columns are not effective
Highly retentive columns in a wide range of selectivities
| Reversed Phase Column Phases | Use to separate samples via hydrophobic interactions between solute molecules in the mobile phase and a hydrophobic stationary phase. C18, C8, C30, Polar Advantage, Polar Advantage II |
| Mixed-Mode Column Phases | Separate polar, nonpolar, and ionizable compounds in a single analysis. Achieve baseline separation of challenging polar analytes without derivatizing. Mixed-Mode WAX-1, Mixed-Mode WCX-1, Mixed-Mode HILIC-1, Trinity P1, Trinity P2, Trinity Q1 |
| Application-Specific Column Phases | Unique stationary phases for specific applications such as the analysis of surfactants, organic acids, pesticides, aminoglycosides, or explosive residues. Organic Acid, Surfactant Plus, AmG C18, Explosives E2, Carbamate, Carbonyl |
| HILIC Column Phases | Ideal for retaining and separating polar compounds based on polarity differences and affinity for a polar stationary phase. HILIC-10 |
Phase Name | 2.2 µm | 3 µm | 5 µm | 7 µm | Pore Size (Å) | Surface Area (m²/g) | Carbon Load (%) | USP |
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| — | 120 | 300 | 18 | L1 | |
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| — | — | 300 | 100 | 8 | L1 | |
| — | — | — | 120 | 300 | 18 | L1 | |
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| — | 120 | 300 | 11 | L7 | |
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| — | 120 | 300 | 17 | L60 | |
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| — | 120 | 300 | 17 | L60 | |
| — | — | — | 120 | 300 | 17 | L60 | |
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| | — | 200 | 200 | 13 | L62 | |
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| — | 120 | 300 | 13 | L11 | |
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| — | — | 120 | — | 8 | — | |
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| — | 120 | 300 | 17 | — | |
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| — | 120 | 300 | 12 | — | |
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| — | 120 | 300 | — | — | |
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| — | 120 | 300 | — | — | |
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| — | 120 | 300 | — | — | |
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| — | 120 | 300 | — | — | |
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| — | — | 300 | 100 | — | — | |
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| — | — | 120 | 300 | — | — | |
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| — | — | 300 | 100 | — | — | |
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| — | — | 300 | 100 | — | — | |
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| — | 120 | 300 | — | L85 | |
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| — | 120 | 300 | — | — | |
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| — | 120 | 300 | — | L78 | |
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| — | 300 | — | — | L25 |
Learn more about Acclaim columns
Resources
HPLC column selection guide
3 ways to search
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Know your options
A great resource for getting familiar with and comparing (U)HPLC columns, our Premier Columns Guide contains:
- Overview of column chemistries
- Column comparison chart
- Solid core versus fully porous supports
- Pore size versus surface area
- Quick guide to choosing a stationary phase
- Ordering information
Download premier columns guide
Hypersil GOLD (U)HPLC column literature
Accucore (U)HPLC column literature
Acclaim (U)HPLC column literature
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