Search Thermo Fisher Scientific
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Identified biomarkers from either untargeted metabolomics experiments, or hypothesis-driven from the literature, can be verified or validated where usually a limited number of compounds are quantified in a large set of samples.
For highest selectivity and confirmation, HRAM Orbitrap mass spectrometry is the technology of choice for verification of putative biomarkers. Moving through to high-throughput quantitation, SRM analysis on the TSQ triple quadrupole mass spectrometers is the solution of choice.
Targeted metabolomics offers researchers the possibility to quantify selected metabolites. The AbsoluteIDQ® p400 HR Kit is the first standardized solution for broad metabolic and lipid profiling developed for Thermo Scientific high-resolution, accurate mass (HRAM) Orbitrap MS. It covers hundreds of metabolites while providing quantification, reproducibility, and high throughput. The kit offers the opportunity to analyze various biological matrices from multiple species.
Samples are registered in the MetIDQ™ software (integral part of the kit). The test mix is run to ensure suitable system conditions.
Samples and reagents are pipetted onto the kit plate
Amino acids and biogenic amines are derivatized to help ensure a reliable analysis.
Metabolites are extracted with organic buffer and diluted in two steps for subsequent mass spectrometric analysis.
Obtain valuable quantitative insights from small- to large-scale studies across a variety of applications from untargeted, semi-targeted to targeted metabolomics studies. With optimized methods, the Thermo Scientific Orbitrap Exploris 240 mass spectrometer delivers a fast turnaround of sample to results with operational simplicity. Best-in-class performance together with Thermo Scientific AcquireX intelligent data acquisition delivers a fast track to confident results, all within a compact footprint, so you can go beyond with everyday versatility.
MetIDQ™ highly automates the whole FIA-MS and LC-MS process from project and sample administration to plate layout and work list generation, technical validation and statistical analysis to standardize metabolomics project data processing. MetIDQ is an integral part of all AbsoluteIDQ® Kits.
Samples are registered in the MetIDQ™ software (integral part of the kit). The test mix is run to ensure suitable system conditions.
Samples and reagents are pipetted onto the kit plate
Amino acids and biogenic amines are derivatized to help ensure a reliable analysis.
Metabolites are extracted with organic buffer and diluted in two steps for subsequent mass spectrometric analysis.
Obtain valuable quantitative insights from small- to large-scale studies across a variety of applications from untargeted, semi-targeted to targeted metabolomics studies. With optimized methods, the Thermo Scientific Orbitrap Exploris 240 mass spectrometer delivers a fast turnaround of sample to results with operational simplicity. Best-in-class performance together with Thermo Scientific AcquireX intelligent data acquisition delivers a fast track to confident results, all within a compact footprint, so you can go beyond with everyday versatility.
MetIDQ™ highly automates the whole FIA-MS and LC-MS process from project and sample administration to plate layout and work list generation, technical validation and statistical analysis to standardize metabolomics project data processing. MetIDQ is an integral part of all AbsoluteIDQ® Kits.