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MycoSEQ Mycoplasma Detection System
The Applied Biosystems MycoSEQ Mycoplasma Detection System is a fully integrated, real-time PCR (qPCR) solution for rapid Mycoplasma testing. Used throughout the bioproduction workflow, the MycoSEQ System supports an appealing alternative to time-consuming culture-based tests by helping to deliver accurate and actionable results in about five hours.
Backed by a successful track record of regulatory acceptance, the MycoSEQ System can help simplify Mycoplasma detection for raw material, cell bank, in-process, and lot-release testing.
- Method accepted by regulatory agencies for Mycoplasma detection in a cGMP environment
- Requires no live Mycoplasma for testing or process validation
- Total workflow solution, from sample prep to analysis with SAE compatible software
- Detection of all recommended species listed in European, US, and Japanese pharmacopeias
- Supported throughout the validation process
Simple qPCR workflow
The complete MycoSEQ Mycoplasma Detection System is an integrated, real-time PCR (qPCR) solution for rapid mycoplasma testing. Comprised of functionally confirmed sample-to-answer protocols, commercially available kits for sample preparation and qPCR, and instruments with analytical software to help enable regulatory compliance, the MycoSEQ workflow enables a flexible approach to meet a variety of Mycoplasma testing applications.
Superior Mycoplasma extraction
PrepSEQ 1-2-3 Mycoplasma Nucleic Acid Extraction Kit
Optimized for use with MycoSEQ Mycoplasma Detection Kits, the PrepSEQ 1-2-3 Mycoplasma Nucleic Acid Extraction Kit uses proprietary magnetic bead–based separation technology to extract Mycoplasma DNA from mammalian cell lines. The PrepSEQ 1-2-3 kit extracts Mycoplasma genomic DNA from research cell lines, stem cell lines, and cellular and tissue therapy samples with high efficiency and high recovery—helping to deliver results in under 2 hours.
AutoMate Express Nucleic Acid Extraction System
An easy-to-use benchtop instrument enables hands-free automation of the nucleic acid extraction process for the MycoSEQ Mycoplasma detection workflow. The instrument uses the established PrepSEQ nucleic acid extraction chemistry packaged into a cartridge format for quantitative recovery of DNA and RNA. The AutoMate Express system offers out-of-the-box automation for easy integration into existing workflows and lab configurations. There is minimal hands-on time with this closed system—just load and go with prefilled reagent cartridges and preprogrammed protocols.
Rapidly detect Mycoplasmas with confidence
Core to the MycoSEQ System are its high-performance assay kits. MycoSEQ Mycoplasma detection kits are available in both probe-based and dye-based formulations to enable optimum performance, regardless of sample matrices. With a long track record of validation, MycoSEQ Solutions have been accepted worldwide as meeting regulatory guidelines for the detection of Mycoplasmas across multiple therapeutic modalities.
Leading real-time PCR technology
With more than 25 years of qPCR innovation, Thermo Fisher is a recognized leader in qPCR instrumentation. Applied Biosystems qPCR systems help deliver true value with excellent performance, reliability, and world-class support.
The MycoSEQ Mycoplasma Detection Assays have been functionally validated on the Applied Biosystems QuantStudio 5 Real-Time PCR System. A high-performance benchtop instrument, the QuantStudio 5 System gives you greater control of your experimental data. Interact with the latest advancements in touchscreen usability, access your data more easily than ever before, and built-in SAE to help enable 21 CFR Part 11 compliance.
Streamlined data interpretation for fast and accurate results
Applied Biosystems AccuSEQ Real-Time PCR Software offers efficient and streamlined data interpretation for analyzing contaminants and impurities in various testing scenarios. Serving as a pivotal element within an all-encompassing Mycoplasma detection solution, AccuSEQ software equips you with actionable results crucial for biopharmaceutical manufacturing processes.
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