Gibco Cell Therapy Bioprocessing Media | Thermo Fisher Scientific

Gibco CTS OpTmizer Pro serum-free media formatted in three different sizes – BPCs, bottles, or vials

Advancing your cell therapy development workflow

When scaling up a T-cell manufacturing process for clinical and commercial manufacturing, it is important to select one that is specifically made for the cell type you are using. Used in >200 clinical trials and in commercialized therapies, the Gibco CTS OpTmizer family of serum-free media (SFM) is thoughtfully designed to meet the nutritional needs of high-density T-cells, which is particularly important when scaling up. The result is that all our media can help maintain the desired central memory phenotype. In addition, whether you’re developing an autologous or allogeneic T-cell therapy, CTS OpTmizer SFM are serum and xeno free and have extensive traceability documentation, including Drug Master Files and Regulatory Support Files.

Thermo Fisher Scientific is committed to helping you evolve the future of your cell therapy manufacturing needs

What’s new: Gibco CTS OpTmizer One Serum-Free Medium (SFM)

The OpTmizer One SFM is a novel animal origin–free formulation that can help improve process consistency and scalability while showing high cell viability, improved growth, and early memory T-cell phenotype.

Key features:

No separate components: No need to mix basal medium with supplement to obtain complete medium
Superior performance: Improved early memory T-cell phenotype, increased cell growth, high cell viability, helping to improve drug function
Animal origin–free (AOF) formulation: Reduced risk of contaminants and variability from animal-source components
Superior performance: Enhanced T-cell expansion, early memory phenotype, and function

Gibco Cell Therapy Media Product Offerings

CTS OpTmizer One SFM

Material used at every stage of a cell therapy workflow can impact the efficacy of the final drug. Gibco CTS OpTmizer One SFM takes this into account with thoughtfully designed nutrition for T-cells. Not only does CTS OpTmizer One SFM provide the nutrients and energy for T-cell proliferation, it does so without any animal components. This novel animal origin-free formulation helps mitigates the risk of contamination from adventitious agents with superior performance compared to other chemically defined media.

This AOF formulation also demonstrates flexibility and scalability across culture vessels—G‑Rex™ plates, rocking bioreactors, and stir tank bioreactors—CTS OpTmizer One SFM supports scale up while maintaining the desired phenotype. To facilitate scalability, CTS OpTmizer One SFM come in 1 L, 5 L, and 10 L Thermo Scientific Aegis5-14 Film bioprocess containers, which allows compatibility with closed systems.

Higher cell count in donors with use of CTS OpTmizer One SFM

Donors achieve more consistent expansion with CTS OpTmizer One SFM

Figure 1. Fold expansion. (Top graph) Comparable or higher cell growth (bars) was consistently demonstrated in healthy donors (dots) with CTS OpTmizer One SFM relative to media from three different suppliers (CM1, CM2, and CM3). (Bottom graph) By day 10, acute myeloid leukemia (AML) donor cells achieved more consistent and robust expansion (284-fold, as indicated by bars) with CTS OpTmizer One SFM compared to media from other suppliers (CM1, CM2, and CM3).

Ordering information

CTS OpTmizer T Cell Expansion SFM, no phenol red

Gibco CTS OpTmizer T-Cell Expansion SFM, no phenol red is serum-free, xeno-free. Developed for the growth and expansion of human T lymphocytes for autologous therapies. This complete serum-free and xeno-free medium has demonstrated successful T cell expansion in cell culture plates up to large-scale bioreactors as effectively as serum-containing medium.

Graph showing the faster fold expression time of a stirred tank bioreactor compared to rocking and static bioreactors

Figure 2. Fold expansion comparison of T cells in three different bioreactors. Increased fold expansion was achieved in the stirred-tank bioreactor, followed by the rocking motion bioreactors and static bioreactors.

Ordering information

CTS OpTmizer Pro SFM

Gibco CTS OpTmizer Pro SFM is a novel medium, developed for the growth and expansion of human T lymphocytes (e.g., CD4+, CD8+, polyclonal cells, or antigen specific T cells) used in allogeneic cell therapies. CTS OpTmizer Pro SFM improves central memory phenotype and cell growth by shifting the cell’s metabolism. The result is a larger population of central memory cells in a shorter period of time.

CTS OpTmizer Pro SFM come in Thermo Scientific Aegis5-14 Film, which allows compatibility with closed systems. Thermo Scientific Aegis5-14 Film is a five-layer cast film produced in a cGMP facility. The outer layer is a polyester elastomer coextruded with an ethyl vinyl alcohol (EVOH) barrier layer and an ultra-low-density polyethylene product contact layer. Aegis5-14 Film is manufactured using no animal derived components.

Figure 3. T cell proliferation in healthy donors evaluated using Gibco CTS OpTmizer Pro SFM. Enhanced cell growth was observed when cultured in the presence of CTS OpTmizer Pro SFM. Cells from six healthy donors were expanded in CTS OpTmizer Pro SFM. Cells were assessed at day 17. Improved growth that became more pronounced over time was observed as indicated by an average increase of 187% compared to Gibco CTS OpTmizer SFM, no phenol red.