Attune Flow Cytometer Resources

“We’ve been very comfortable working with Thermo Fisher Scientific for the last four years. We have a collaboration arrangement that goes back over three and a half years and has taken us from initial, in some cases, accidental/serendipitous discoveries, to a point where the technology we’re working on is being adopted by internationally respected reference laboratories overseas. That’s very unusual for a group based in somewhere as geographically isolated as Western Australia. This collaboration is a very unusual one, at least, for us locally and has been a very productive one.”

“One of the problems that everyone is familiar with who works in flow cytometry is clogging. Clogging is a thing of the past with this instrument. You have so many samples that you have to run, you can’t wait between samples to clear everything out.”

"I can't believe how quickly and easy is to collect a large body of empirical evidence on the Attune NxT to fully support our hypothesis."

“This Attune NxT Small Particle SSC Filter has greatly increased the sensitivity of the system.  It really does make a huge difference.”

“So after evaluating several platforms, we chose the NxT because of its speed. It is able to analyze cells at a much higher rate, with very good precision, and it allows us to answer questions that other systems have trouble with, especially in the exosome field. The high resolution has been very beneficial in helping us resolve individual exosomes which are extremely small compared to other types of cells.”

“The analysis of minor population cells, such as the analysis of progenitor cells, is essential for immunology research today. As the sample volume inevitably increases and samples are so valuable, we need to use many fluorescently labeled antibodies in a single experiment. The Attune NxT Flow Cytometer that we primarily used in this case can detect up to a maximum of 14 colors using four colored lasers. I consider it to be a researcher-friendly system.” 

“What I like most about the Attune NxT [Flow Cytometer] is its accuracy and its speed of processing. I could list a range of other things that I like about it because we are building up that list as we discover more aspects of its functions, but the core things are speed and accuracy that are not available with hydrodynamic flow cytometers.”

“What we like about the Attune NxT in our lab, is the speed and effectiveness it provides for our cytokine and hormone bead-based assays. We run both 96 and 384 well bead assays, typically 10-15 plex, and the Attune NxT is a real workhorse. We combine it with our MPLEX software which reduces a complete plate of data in about a minute.”

“The Attune NxT autosampler allowed us to process multiple 96-well plates in a day, enabling high throughput experiments that could not be performed on other flow cytometers.”

“The Attune NxT software is very user friendly and intuitive, significantly reducing training time required to get new users up-and-running.”

“The Attune NxT is a very well-built instrument, we are very happy with the Attune NxT. The automated Autosampler is a real plus when it is combined with the acoustic focusing because it allows you to count very diluted samples fast. It’s very precise and gives reliable event counting.”

“I would highly recommend the Attune NxT cytometer.  It has several advantages including speed and ease of use, and it rarely clogs!  They also have an excellent plate/tube loader.  The best thing is that you can start with only one laser and easily field upgrade in just one afternoon, to add more in the future.”