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The DH10B strain of E. coli is a popular strain for everyday cloning applications due to its high transformation efficiency, its ability to uptake and maintain large plasmids (>30 kb), lack of methylation-dependent restriction systems (MDRS), and blue-white colony screening. DH10B can intake genomic DNA which differentiates it from the DH5α strain. The full DH10B genome was sequenced and published by Durfee et al. in 2008 [1].
MAX Efficiency DH10B Competent Cells | MAX Efficiency DH10B T1 Phage Resistant Cells* | ElectroMAX DH10B Cells | ElectroMAX DH10B T1 Phage-Resistant Competent Cells | MegaX DH10B T1R Electrocomp Cells | |
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Transformation efficiency | >1 x 109 cfu/µg | >1 x 109 cfu/µg | >1 x 1010 cfu/µg | >1 x 1010 cfu/µg | >3 x 1010 cfu/µg |
Type of transformation | Chemical | Chemical | Electroporation | Electroporation | Electroporation |
Resistance to T1, T5, and φ80 phage contamination | –– | ✓ | –– | ✓ | ✓ |
Available formats |
* Strain also available in high-throughput FlexPlate format.
In addition to competent cells, we offer a broad suite of products for the cloning workflow.
Explore types of bacterial growth media to use for cloning, plasmid DNA preparation, and protein expression.
Learn more about universal-fit electroporation cuvettes for use with competent cells.
Learn more about one-step PCR cloning that combines traditional restriction and ligation cloning.
Gibson Assembly cloning kits provide highly efficient, seamless cloning, enabling the assembly of multiple DNA fragments of varying lengths into any vector.
Have any questions on competent cells or transformation? Click on the resources listed below to access overviews, videos, genotype guides, and educational resources.
Review rich educational content on transformation workflow, troubleshooting, strategies, and selection guides.
Stay up-to-date on industry trends in molecular cloning with on-demand webinars.
Access white papers and technical and application notes on molecular cloning.
Watch instructional videos on troubleshooting tips, how-to videos, and explainers related to molecular cloning.
For Research Use Only. Not for use in diagnostic procedures.