Oligonucleotide Ordering and Delivery

A universal characteristic of chemical synthesis of oligonucleotides is that the final yield is less than the starting synthesis scale. Despite coupling efficiencies greater than 99%, the iterative addition of each base in the oligo chain compounds the loss of potential full-length material you can achieve. The expected final yield is, therefore, dependent on the length of the oligo. In addition, some material loss also occurs during processing, purification, and quality control. For an example of how coupling efficiency affect final yield, see our FAQ on this topic.

Table 1 shows the estimated nmol yield for desalted oligos at each starting synthesis scale. This table converts the OD units into nmol values for an oligo with an equal and even distribution of each base. The actual nmol conversion from OD for a specific oligo may be different based on the composition and distribution of each base within the oligo.

When ordering custom oligos, remember that the scale of synthesis (25 nmol, 50 nmol, 200 nmol, 1 µmol, or 10 µmol) is the starting point for synthesis, not the guaranteed final amount. Oligonucleotide length, sequence, GC content, and coupling efficiencies can vary the yield of nmol amounts post-synthesis.

Note: We guarantee the total yield of oligonucleotide as a minimum number of OD units. Purification by HPLC, PAGE, and Cartridge will also affect final product yield.

Table 2 below shows minimum guarantee yields (OD₂₆₀). Please note that OD guarantee is valid for unmodified oligos 20 to 100 bases in length.

Yields for oligos under 20 bases are not guaranteed. In general, they are about half of the values as shown in the table below.

For pricing, please contact your local office or distributor. For next-day service, orders can only be placed online.

Prices are included for your planning purposes only and may be subject to change. A shipping/handling charge of $12.50 per order will be applied. For all inquiries, please email us.

For plate orders containing oligos of 50 bases or less.

For any 3′ or 5′ modifications, add 2 business days to each category.
For plate orders containing oligos greater than 50 bases in length, add 2 business days to each category.

• Standard overnight shipping
• Dried oligo—Ambient shipping Monday through Friday
• Resuspended oligo—Frozen shipping Monday through Thursday or ambient shipping Monday through Friday