Find a master mix for your real-time qPCR application

For ease of selection, our real-time qPCR master mixes are organized into two general categories: qPCR master mixes (DNA input) and one-step RT-qPCR master mixes (RNA input).

Real-time qPCR master mixes

­­1-step RT-qPCR master mixes (RNA input)

qPCR master mixes (DNA input)


TaqPath DuraPlex 1-Step RT-qPCR Master Mix

TaqPath 1-Step RT-qPCR Master

TaqPath BactoPure Microbial Detection Master Mix


TaqPath ProAmp Master Mix


TaqPath qPCR Master Mix, CG

­Recommended applications

1-step gene expression

Pathogen detection

1-step gene expression

DNA microbial detection

SNP genotyping

Copy number variation

2-step gene expression
microRNA analysis

Target/input

RNA RNA

DNA

gDNA

cDNA

Concentration

4X 4X

2X

2X

2X

Multiplexing and passive reference dye

Available in two versions:
With ROX (up to 5 targets)
No ROX (up to 6 targets)
Available in three versions:
With ROX (up to 5 targets)
No ROX (up to 6 targets)
With Mustang purple (>3 targets)

Available in two versions:
With ROX (≤3 targets)
No ROX (>3 targets)

Available in two versions:
With ROX (≤3 targets)
With Mustang Purple (>3 targets)

With ROX (up to 3 targets)

Reverse transcriptase

Thermostable MMLV RT included Thermostable MMLV RT included

N/A

N/A

Purchase RT separately and use before qPCR

Cycling mode/run time

Standard: ~110 min
Fast: ~60 min
Standard: ~110 min
Fast: ~60 min

Standard: ~120 min
Fast: ~50 min

Standard: ~120 min
Fast: ~50 min

Standard: ~50 min
Fast: ~40 min

Carryover contamination

UNG compatible (UNG not included) With UNG

With UNG

With UNG

With UNG

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TaqPath master mixes are general purpose reagents labeled ‘For Laboratory Use.’