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Endogenous control genes, also called housekeeping genes, are an essential component of relative gene expression experiments. A housekeeping gene has expression levels that are relatively constant and moderately abundant across tissues, cell types, and treatment protocols. Normalizing observed gene expression changes to an endogenous control is currently the most accurate method to resolve potential biases caused by:
No single gene can act as a universal endogenous control for all experimental conditions, so we recommend verifying the chosen housekeeping gene or set of housekeeping genes for the sample tissue, cell, or treatment. TaqMan Endogenous Control Assays are a collection of assays targeting housekeeping genes that are commonly used in quantitative PCR gene expression experiments.
Use our Assay Search Tool to perform a more thorough search for all assays targeting a certain gene or use the Endogenous Control Selection tool to see what your peers have used for similar applications.
Use this tool to help identify potential housekeeping genes for your experiment by seeing which endogenous controls your peers have successfully used for similar applications.
If there is uncertainty around which endogenous control is most accurate for a specific experiment, endogenous control candidates may be tested in an easy, economical way using TaqMan arrays. We offer endogenous control panels in standard 96-well array plates, Fast 96-well array plates, and TaqMan array cards. These arrays may be purchased individually, maximizing flexibility to test as few or as many samples as needed. Once the candidate gene with the lowest variation has been identified from the array data, the individual TaqMan Endogenous Control Assay for that gene may be purchased for the final experiment.
For Research Use Only. Not for use in diagnostic procedures