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miRNA Analysis and Real-Time PCR (qPCR) Information
MicroRNAs are single-stranded, 19- to 25-nucleotide RNAs that regulate gene expression post-transcriptionally by binding to the 3’ untranslated regions (UTRs) of target mRNAs, inhibiting their translation. The high relative stability of miRNA in biofluids (e.g., plasma, serum, urine, saliva, etc.) and the ability of miRNA expression profiles to accurately classify discrete tissue types and disease states have positioned miRNA quantification as a promising new tool for a wide range of biomarker discoveries. MicroRNAs are gaining popularity in emerging applications such as early detection of cancer, cardiovascular diseases, and simultaneous detection of miRNA and mRNA from the same samples for pathway analysis, etc.
Featured resources
Video
Expression analysis of both mRNA and miRNA on the same TaqMan Array card
This webinar focuses on pancreatic cancer research using the simultaneous measurement and analysis of mRNA and miRNA in the same sample.
Videos
Get answers to the most frequently asked qPCR questions with our library of quick and easy educational videos covering a variety of topics including experiment setup, best practices, and guidance on products and technologies.
Overview of Noncoding RNA & microRNA
This article covers microRNA biogenesis and regulation, cellular function and disease, target prediction and validation.
How TaqMan Assays Work
A brief explanation and illustration of how the TaqMan chemistry works in the various applications of gene expression, genotyping, copy number variation, microRNA, mutation detection, and protein quantification.
Webinar: Analyze microRNAs in limited FFPE samples
A video that discusses comparison of small RNA sequencing and RT-qPCR profiles from nevus and melanoma FFPE samples.
Video: Normalization methods of miRNA quantification
A short video that explains the purpose of normalization and endogenous controls, exogenous controls, and mean expression methods.
A complete workflow for high-throughput isolation of serum miRNAs and downstream analysis by qRT-PCR: application for cancer research and biomarker discovery
This application note describes the workflow, reagents, and tools for recovery and analysis of circulating miRNAs from blood samples.
High correlation of miRNA quantitation data from matched FFPE and snap-frozen tissues using TaqMan MicroRNA Assays
Discusses the isolation and detection of miRNAs from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) samples and demonstrates the accurate and robust measurement of miRNA expression from comparable snap-frozen samples.
Real-Time PCR: Understanding Ct
Learn more about Ct (threshold cycle), the intersection between an amplification curve and a threshold line. It is a relative measure of the concentration of target in the PCR reaction.
TaqMan Advanced miRNA Assays—superior performance for miRNA detection and quantification
This whitepaper highlights the TaqMan probe based performance for detecting low-abundance miRNAs, measurement of small fold-changes in serum, and single-base discrimination of related miRNAs.
A technical guide to identifying miRNA normalizers using TaqMan Advanced miRNA Assays
Discussion of a workflow to identify the two or three most stable miRNAs in a set of samples and use them as endogenous controls to normalize miRNA expression in qPCR studies.
Webinars
Genetic Analysis for All webinars
Learn how genetic analysis techniques, including sequencing, NGS, and quantitative and digital PCR, are being used by researchers to advance knowledge and innovations in challenging disease and research questions.
Video
This video discusses the basic concepts of miRNAs and miRNA research.
Real-Time PCR Resource Library
Innovative tools and educational resources that help advance the work of researchers engaged in real-time PCR.
For Research Use Only. Not for use in diagnostic procedures.