lyo-showcase

Reliable and storage-temperature-agnostic molecular diagnostics qPCR assays need to utilize reagents that are easily lyophilized and provide consistent performance even with non-ideal samples. TaqMan Lyo-Ready qPCR master mixes fulfill these requirements, making them highly suited to lyophilized qPCR assays using your chosen primers and probes.

TaqMan Lyo-Ready qPCR master mixes are designed for consistent performance even in demanding applications, including virus and bacteria detection. Easy to lyophilize and fast to re-hydrate, they ensure consistency before and after lyophilization even in multiplex reactions.

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Download Lyo Ready Whitepaper

TaqMan Lyo-Ready qPCR master mixes are available in several formulations. Customization is also available and can be requested by contacting your Thermo Fisher Scientific representative or emailing custom.solutions@thermofisher.com.

Which TaqMan Lyo-Ready master mix is right for your application?

 

TaqMan 2.5X Lyo-Ready 1-Step Master Mix with excipient

TaqMan 2.5X Lyo-Ready qPCR Master Mix with excipient

TaqMan 5X Lyo-Ready 1-Step Master Mix

TaqMan 5X Lyo-Ready 1-Step Master Mix with ROX

TaqMan 5X Lyo-Ready qPCR Master Mix

TaqMan 5X Lyo-Ready qPCR Master Mix with ROX

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Target

RNA, DNA

DNA

RNA, DNA

RNA, DNA

DNA

DNA

Concentration

2.5X

2.5X

5X

5X

5X

5X

Excipient

Yes, pre-mixed

Yes, pre-mixed

None

None

None

None

Passive reference dye

None

None

None

ROX

None

ROX

Reverse transcriptase

Thermostable M-MLV

N/A

Thermostable M-MLV

Thermostable M-MLV

N/A

N/A

Pre-PCR benchtop stability

4 hr*

4 hr*

<2 hr*

<2 hr*

<2 hr*

<2 hr*

Multiplexing targets

5

5

5

3

5

3

Sample SKU

C14031SMP (1.5 mL)

C14081SMP (1.5 mL)

C1403SMP (1.5 mL)

C1402SMP (1.5 mL)

C1408SMP (1.5 mL)

C1407SMP (1.5 mL)

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This product is best for you if you are

  • New to lyophilization
  • Would like to develop and manufacture a lyophilized qPCR assay
  • Need ready-to-use reagents and comprehensive guidance on the process
  • Experiencedin lyophilization
  • Have your own excipient and lyophilization program
  • Need a qPCR mix optimized for consistent and reliable performance before and after lyophilization

*Recommend setting up plate on ice and running immediately. Stability is assay dependent. Greater stability has been observed for some assays.


TaqMan Lyo-Ready qPCR master mixes are reagents used in further manufacturing (lyophilization) and labeled “Caution: For use as a raw material in further manufacturing applications”, manufactured in an ISO 13485 certified and FDA registered facility, and optimized for exceptional lot-to-lot consistency, inhibitor tolerance, and multiplexing.