Neural Cell Culture Protocols | Thermo Fisher Scientific

Gibco Neurobiology Protocol Handbook

Download a digital copy of more than 20 neurobiology validated protocols for neural stem cell culture and differentiation, cell analysis, molecular characterization, and transfection. Review experimental best practices, and troubleshooting advice designed to get you started with your neurobiology research.

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Browse our Neurobiology protocols. You’ll find the protocols below along with the recommended components to build the optimal neural cell culture system for your research experiment.

Click on the protocol name to view the detailed protocol and full list of required materials.

Click on the product name in the right-hand columns to order.

Neural cell culture and differentiation protocols

Protocol Name	Media	Supplement	Cells
Isolation, Culture, and Characterization of Cortical and Hippocampal Neurons	Neurobasal Plus Medium Hibernate-E Medium	B-27 Plus Supplement
Culturing Human Neural Stem Cells	StemPro NSC SFM media kit (kit includes media, growth factors, and supplement)	GlutaMAX-I Supplement	Human neural stem cells
Differentiating Neural Stem Cells into Neurons and Glial Cells	StemPro NSC SFM media kit (kit includes media, growth factors, and supplement) Neurobasal Medium DMEM	B-27 Plus Supplement B-27 Supplement N-2 Supplement GlutaMAX-I Supplement	Human neural stem cells
Culturing Rat Fetal Neural Stem Cells	StemPro NSC SFM media kit (kit includes media, growth factors, and supplement)		Rat fetal neural stem cells
Derivation and Culture of Cortical Astrocytes	Astrocyte Medium (kit includes medium and supplements)
Cryopreserving Neural Stem Cells	StemPro NSC SFM media kit (kit includes media, growth factors, and supplement)
Cryopreservation and Recovery of Mature Differentiated Neural Cells	Neurobasal Plus Medium	B-27 Plus Supplement

Protocol Name	Media	Supplement	Cells
Isolation, Culture, and Characterization of Cortical and Hippocampal Neurons	Neurobasal Plus Medium Hibernate-E Medium	B-27 Plus Supplement B-27 Supplement
Culturing Human Neural Stem Cells	StemPro NSC SFM media kit (kit includes media, growth factors, and supplement)	GlutaMAX-I Supplement	Human neural stem cells
Xeno-free Culture of Neural Stem Cells	Neurobasal Medium	B-27 Supplement, XenoFree GlutaMAX-I Supplement
Differentiating Neural Stem Cells into Neurons and Glial Cells	StemPro NSC SFM media kit (kit includes media, growth factors, and supplement) Neurobasal Medium DMEM	B-27 Plus Supplement B-27 Serum Free Supplement N-2 Supplement GlutaMAX-I Supplement	Human neural stem cells
Differentiating Glial Precursor Cells into Astrocytes and Oligodendrocytes	Neurobasal Medium Astrocyte Medium (kit includes medium and supplements)	B-27 Plus Supplement B-27 Supplement	Rat glial precursor cells
Derivation of Dopaminergic Neurons (from Human Embryonic Stem Cells)	DMEM DMEM/F-12 with GlutaMAX-I Neurobasal Medium	KnockOut Serum Replacement N-2 Supplement B-27 Plus Supplement B-27 Supplement	Mouse embryonic fibroblasts
Culturing Rat Fetal Neural Stem Cells	StemPro NSC SFM media kit (kit includes media, growth factors, and supplement)		Rat fetal neural stem cells
Derivation and Culture of Dopaminergic Neurons (from Midbrains of Rodents)	Neurobasal Medium	B-27 Plus Supplement B-27 Supplement
Derivation and Culture of Cortical Astrocytes	Astrocyte Medium (kit includes medium and supplements)
Cryopreserving Neural Stem Cells	StemPro NSC SFM media kit (kit includes media, growth factors, and supplement)
Cryopreservation and Recovery of Mature Differentiated Neural Cells	Neurobasal Medium	B-27 Plus Supplement B-27 Supplement

We understand that there’s more to your experiment than culturing your cells. View the protocols below for experiments beyond neural cell culture.

Click on the protocol name to view the detailed protocol and full list of required materials.

Cell analysis

Protocol Name
Cell Viability Assays for Neural Stem Cells
Markers for Characterizing Neural Subtypes
Surface Marker Analysis by Flow Cytometry
Immunocytochemistry
Intracellular Calcium Assay using Fluo-4

Molecular characterization

Protocol Name
PCR Primers for Molecular Characterization of Neural Subtypes
RNA Isolation and cDNA Preparation from Neural Stem Cells
Characterizing Neural Cells by qPCR

Transfection

Protocol Name
Transfecting Neural Cells Using the Neon Transfection System
Lipid-Mediated Transfection of Human Astrocytes

Resources

For Research Use Only. Not for use in diagnostic procedures.