Calcium flux in porcine left atrial appendage progenitor cells transfected with Premo™ Cameleon calcium sensor.

Porcine left atrial appendage progenitor cells were transfected with Premo™ Cameleon calcium sensor (P36207, P36208); ATP (20 µM final concentration) was applied to the cells the following day and the cells were imaged using a Zeiss 5 Live high-speed confocal system (Carl Zeiss MicroImaging). Excitation was with a 405 nm diode laser (50 mw) operated at 50% power. Emission was collected simultaneously on two linear CCD detectors using a 490 nm dichroic mirror to split the beam through a 415–480 nm bandpass filter for CFP and a 550 nm longpass filter for YFP. Images were collected at a rate of 10 frames per second (512 x 512 pixels) using a 40x Plan-Neofluar 1.3 NA oil immersion objective lens.

Porcine left atrial appendage progenitor cells were transfected with Premo™ Cameleon calcium sensor (P36207, P36208); ATP (20 µM final concentration) was applied to the cells the following day and the cells were imaged using a Zeiss 5 Live high-speed confocal system (Carl Zeiss MicroImaging). Excitation was with a 405 nm diode laser (50 mw) operated at 50% power. Emission was collected simultaneously on two linear CCD detectors using a 490 nm dichroic mirror to split the beam through a 415–480 nm bandpass filter for CFP and a 550 nm longpass filter for YFP. Images were collected at a rate of 10 frames per second (512 x 512 pixels) using a 40x Plan-Neofluar 1.3 NA oil immersion objective lens.

Related Products

Related Images

HeLa cells transduced with Organelle Lights™ Mito-GFP. Go ›

Four-color staining of a muntjac cell with probes for cytoskeletal, nuclear and mitochondrial proteins Go ›

REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›

Pseudocolored images of changes in intracellular free Ca2+ in AtT-20/D16v-F2 cells, monitored at 9 sec intervals with fluo-4, AM (F14201, F14202, F14217, F23917). Go ›

Live cell imaging with CellLight™ reagents. Go ›

Live cells transduced with Organelle Lights™ or Cellular Lights™ reagents. Go ›

CD335 (NKp46) Antibody (63335182) in RE Go ›

CD223 (LAG-3) Antibody (56223942) in TM Go ›

Imaging autophagy in live HeLa cells with CellLight® reagents for mitochondria and lysosomes: Go ›

Porcine primary skeletal muscle cell transduced with Organelle Lights fluorescent proteins. Go ›