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Detect multiple targets in a single reaction
Multiplexing is a technique that allows for the simultaneous detection and differentiation of multiple target sequences in a single reaction using real-time PCR (qPCR). Multiplexing involves designing specific primers and probes for each target of interest and optimizing reaction conditions to enable accurate and reliable results. By eliminating the need for individual reactions for each target organism, multiplexing can help provide the advantages of saving time, resources, and costs. With a single reaction, multiple pathogens or genetic markers can be detected, enabling faster turnaround times and helping to streamline and optimize the productivity of your testing process.
Search multiplex panels by research area
Experimental design considerations
qPCR multiplexing, while advantageous, does come with its challenges. One of the main difficulties is the optimization of primer and probe combinations to enable the specific and efficient amplification of each target in the multiplex reaction. Another challenge lies in managing potential cross-reactivity or interference between different targets, which may require additional optimization steps to minimize false positives or inaccurate quantification. Careful experimental design and thorough validation are crucial to overcoming these challenges, helping to support the reliability and accuracy of multiplex qPCR results.
Multiplexing with TrueMark real-time PCR panels
We offer pre-designed and qualified multiplex panels for detecting a broad range of infectious pathogens and resistance genes in research applications for vaginal / women’s health, respiratory infections, sexually transmitted infections, gastrointestinal infections, nail fungal infections, antimicrobial / drug resistance and more.
These panels are made to order and customizable to be reconfigured with your specified targets of interest:
- Package sizes: 200 or 1,000 reactions
- Formats available for predesigned and qualified panels: Single tube, 96- and 384-well pre-spotted plates
What’s the difference between testing levels for “pre-designed” and “qualified” panels?
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Pre-designed panels |
Qualified panels |
In-silico analysis |
No high-risk oligo interactions identified |
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Inclusivity (maximized strain coverage) |
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Additional functional testing |
Analytical sensitivity |
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| Linear dynamic range |
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| Competitive interference (if applicable) |
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Antimicrobial resistance and drug resistance multiplex research panels
| Panel name | Beta-lactamase and Quinolone Resistance | Extended-Spectrum Beta-Lactamase and Quinolone Resistance | Carbapenemase and Beta-Lactamase Resistance | |
| Multiplex ID | Ba00000009_gmplx | Ba00000010_gmplx | Ba00000011_gmplx | |
| Testing level | Pre-designed | Pre-designed | Pre-designed | |
| Targets | Beta-lactamase ampC1 |  |
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| Carbapenem-hydrolyzing class A beta-lactamase KPC (blaKPC) | |
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| Cephalosporin-hydrolyzing class C beta-lactamase blaFOX | blaACC | |
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| Class A beta-lactamase SHV (blaSHV) | |
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| Class A extended-spectrum beta-lactamase CTX-M (blaCTX-M) | |
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| Class A extended-spectrum beta-lactamase PER | |
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| OXA-48 family class D beta-lactamase OXA (blaOXA) | |
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| Quinolone resistance pentapeptide repeat protein (QnrB) | |
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| Quinolone resistance pentapeptide repeat protein QnrA | QnrS | |
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| Subclass B1 metallo-beta-lactamase VIM|NDM|IMP | |
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| Control | Bacillus atrophaeus | |
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Gastroenteric multiplex research panels
| Panel name | Astrovirus, Adenovirus, ETEC (Enteric) | Yersinia, Clostridium difficile (Enteric) | Bacteroides, Enterovirus, Vibrio (Enteric and Environment) | Sapovirus (Gastroenteritis) | |
| Multiplex ID | Pa00000012_gmplx | Ba00000014_gmplx | Pa00000013_gmplx | Vi00000018_gmplx | |
| Testing level | Pre-designed | Pre-designed | Pre-designed | Pre-designed | |
| Targets | Adenovirus F40/41 | |
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| Astrovirus | |
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| Bacteroides fragilis | |
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| Clostridium difficile toxin A/B | |
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| Enterotoxigenic E. coli (ETEC) | |
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| Enterovirus | |
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| Sapovirus GII.5 / GII.8 / GIV.1 / GV | |
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| Sapovirus I / GII / IV | |
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| Vibrio cholerae / Vibrio parahaemolyticus / Vibrio vulnificus | |
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| Yersinia enterolitica | |
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| Control | Bacillus atrophaeus | |
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Nail fungal multiplex research panels (with Bacillus atrophaeus)
| Panel name | Fusarium, Trichophyton, Aspergillus, Candida - BA | Trichophyton, Candida, Aspergillus - BA | Trichophyton, Candida, Fusarium - BA | Trichophyton, Candida, Scopulariopsis - BA | Trichophyton, Fusarium, Scopulariopsis, Candida - BA | |
| Multiplex ID | Fn00000020_gmplx | Fn00000024_gmplx | Fn00000025_gmplx | Fn00000026_gmplx | Fn00000028_gmplx | |
| Testing level | Pre-designed | Pre-designed | Pre-designed | Pre-designed | Pre-designed | |
| Aspergillus niger / Aspergillus terreus | |
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| Candida albicans / Candida parapsilosis / Candida tropicalis / Candida glabrata | |
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| Fusarium solani | |
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| Scopulariopsis brevicaulis | |
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| Trichophyton soudanense | |
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| Trichophyton spp | |
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| Control | Bacillus atrophaeus | |
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| Panel name | Dermatophyte 1 - BA | Non-Dermatophyte 1 - BA | Candida Differentiation - BA | |
| Multiplex ID | Fn00000030_gmplx | Fn00000033_gmplx | Fn00000034_gmplx | |
| Testing level | Pre-designed | Pre-designed | Pre-designed | |
| Targets | Alternaria spp. | |
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| Aspergillus niger / Aspergillus terreus / Aspergillus flavus / Aspergillus fumigatus | |
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| Candida albicans | |
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| Candida glabrata | |
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| Candida parapsilosis | |
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| Candida tropicalis | |
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| Epidermophyton floccosum | |
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| Fusarium solani | |
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| Microsporum | |
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| Scopulariopsis brevicaulis | |
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| Trichophyton mentagrophytes complex | |
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| Trichophyton soudanense | |
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| Control | Bacillus atrophaeus | |
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Nail fungal multiplex research panels (with RNase P)
| Panel name | Fusarium, Trichophyton, Aspergillus, Candida - R | Trichophyton, Candida, Aspergillus - R | Trichophyton, Candida, Fusarium - R | Trichophyton, Candida, Scopulariopsis - R | Trichophyton, Fusarium, Scopulariopsis, Candida - R | |
| Multiplex ID | Fn00000019_gmplx | Fn00000021_gmplx | Fn00000022_gmplx | Fn00000023_gmplx | Fn00000027_gmplx | |
| Testing level | Pre-designed | Pre-designed | Pre-designed | Pre-designed | Pre-designed | |
| Aspergillus niger / Aspergillus terreus | |
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| Candida albicans / Candida parapsilosis / Candida tropicalis / Candida glabrata | |
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| Fusarium solani | |
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| Scopulariopsis brevicaulis | |
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| Trichophyton soudanense | |
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| Trichophyton spp | |
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| Control | RNase P | |
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| Panel name | Dermatophyte 1 - R | Non-Dermatophyte 1 - R | Candida Differentiation - R | |
| Multiplex ID | Fn00000029_gmplx | Fn00000031_gmplx | Fn00000032_gmplx | |
| Testing level | Pre-designed | Pre-designed | Pre-designed | |
| Targets | Alternaria spp. | |
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| Aspergillus niger / Aspergillus terreus / Aspergillus flavus / Aspergillus fumigatus | |
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| Candida albicans | |
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| Candida glabrata | |
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| Candida parapsilosis | |
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| Candida tropicalis | |
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| Epidermophyton floccosum | |
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| Fusarium solani | |
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| Microsporum | |
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| Scopulariopsis brevicaulis | |
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| Trichophyton mentagrophytes complex | |
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| Trichophyton soudanense | |
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| Control | RNase P | |
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Respiratory multiplex research panels
| Panel name | Flu A, Flu B, RSV | Parainfluenza differentiation | Bordetella differentiation (Whooping cough) | Coronavirus differentiation | FluA, FluB, FluA H5N1, SARS‑CoV‑2 | |
| Multiplex ID | Vi00000052_gmplx | Vi00000045_gmplx | Ba00000054_gmplx | Vi00000053_gmplx | Vi00000181_gmplx | |
| Testing level | Qualified | Qualified | Qualified | Pre-designed | Qualified | |
| Targets | Bordetella holmesii | |
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| Bordetella parapertussis | |
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| Bordetella pertussis | |
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| Coronavirus OC43 | |
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| Coronavirus HKU1 | |
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| Coronavirus 229E | |
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| Coronavirus NL63 | |
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| Influenza A | |
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| Influenza A H5N1 | |
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| Influenza B | |
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| Parainfluenza 1 | |
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| Parainfluenza 2 | |
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| Parainfluenza 3 | |
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| Parainfluenza 4 | |
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| RSV | |
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| SARS-CoV-2 | |
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| Control | RNase P | |
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Sexually transmitted infections (STI) multiplex research panels
| Panel name | Herpes Simplex | HPV High Risk | HPV Low Risk 1 | HPV Low Risk 2 | |
| Multiplex ID | Vi00000004_gmplx | Vi00000005_gmplx | Vi00000006_gmplx | Vi00000007_gmplx | |
| Testing level | Qualified | Pre-designed | Pre-designed | Pre-designed | |
| Targets | HSV 1 | |
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| HSV 2 | |
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| HPV 16 | |
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| HPV 18 | |
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| HPV 31/51 | |
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| HPV 33/35 | |
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| HPV 39/58 | |
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| HPV 45 | |
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| HPV 52/56 | |
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| HPV 59/66 | |
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| HPV 68 | |
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| Control | RNase P | |
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Vaginal and women’s health multiplex research panels
| Panel name | Vaginal pathogen | Vaginal profile | Vaginal candida | |
| Multiplex ID | Pa00000001_gmplx | Ba00000002_gmplx | Fn00000041_gmplx | |
| Testing level | Qualified | Qualified | Qualified | |
| Targets | Atopobium vaginae | |
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| Candida dubliniensis / Candida albicans / Candida parapsilosis / Candida tropicalis | |
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| Candida glabrata | |
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| Candida glabrata / Candida krusei | |
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| Candida krusei | |
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| Gardnerella vaginalis | |
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| Lactobacillus jensenii / Lactobacillus crispatus / Lactobacillus gasseri | |
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| Megasphaera 1 | |
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| Trichomonas vaginalis | |
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| Control | RNase P | |
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Related resources
For Research Use Only. Not for use in diagnostic procedures.