ELISA Kits

Novex® ELISA kits allow specific, quantitative measurements of disease-related proteins, including cytokines, chemokines, beta amyloids, and signaling targets.

  • Sensitive, accurate, and consistent performance
  • Validated on serum, plasma, tissue culture supernant, or lysate
  • Ready-to-use, convenient assay in only half a day



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About Novex® ELISA Kits

ELISA Kits Ready to Use
Novex® ELISA test kits come ready to use with a precoated 96-well plate and all necessary reagents. A detailed, easy-to-follow protocol with kit-specific performance claims is also provided.  Just add sample, run the assay, and get quantitative results in half a day (Figure 1).

Our ELISA kits help provide accurate and consistent results (Figure 2). We research each target protein and calibrate our ELISA kits to provide physiologically relevant sensitivity. In addition, kits are validated using common sample types including serum, plasma, supernatant, and cell lysates for signaling or phosphorylation proteins.
 
ELISA kits are manufactured to help ensure excellent quality and reproducibility. Kits must meet quality-controlled specifications for sensitivity, dynamic range, precision, specificity, recovery, and lot-to-lot consistency in order to leave our facilities. Our stringent quality specifications require that each new lot of ELISA kits exhibits <20% variation compared to previous lots. 

ELISA Kit Protocol
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Figure 1. Fast and easy, 4-hour Novex® ELISA kit protocol.  Capture antibodies are precoated on the bottom of the 96-well plate. The sample or standard is added to the wells and incubated to allow target proteins to bind. The wells are then washed to remove unbound material and the detection antibody is added and incubated to form a sandwich around the protein of interest. HRP conjugate is then added and incubated to allow binding via a biotin–streptavidin interaction. Next, the chromogen substrate for HRP is added and the subsequent enzymatic reaction turns the solution blue. Finally, the reaction is stopped, turning the solution yellow in proportion to the amount of target protein in the sample. Results are read in a microplate reader at 450 nm.

For Research Use Only.  Not intended for any animal or human therapeutic or diagnostic use.


IFN-gamma Human ELISA Kit
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Figure 2. Novex® ELISA kits provide reproducible results from lot to lot. Individual production lots (A–K) of the Novex® IFN-γ Human ELISA Kit were analyzed using 4 concentrations of control target protein (C1–C4). All 11 lots provided uniform sensitivity and low lot-to-lot variation (<20%).