Transfection Reagents | Thermo Fisher Scientific - CA

Life Technologies™ transfection reagents offer options for DNA, siRNA, oligonucleotide, and RNA delivery, giving you more confidence in your results.

Superior delivery—transfect a broad range of cell types, including those notoriously difficult to transfect, with high efficiency
Nontoxic performance—you see results due to the presence of your nucleic acid, not the reagent
Less optimization—rapid, simple protocols provided for most cell lines

Transfection reagent selection guide

Cell Lines
Primary Cells

Reagent	DNA	mRNA	RNAi	Co- delivery	Easy-to-transfect adherent cells	Hard-to-transfect adherent cells	Suspension cells
NEW Lipofectamine® 3000
Lipofectamine® RNAiMAX
Lipofectamine® 2000
Lipofectamine® LTX
Neon® Electroporation
Invivofectamine® 2.0					N/A	N/A	In vivo

Reagent	DNA	mRNA	RNAi	Co- delivery	Neurons	Stem Cells	Blood Cells	Others
NEW Lipofectamine® 3000
Lipofectamine® RNAiMAX
Lipofectamine® 2000
Lipofectamine® LTX
Neon® Electroporation
Invivofectamine® 2.0					N/A	N/A	In vivo

Reagent	DNA	mRNA	RNAi	Co- delivery	Easy-to-transfect adherent cells	Hard-to-transfect adherent cells	Suspension cells
NEW Lipofectamine® 3000
Lipofectamine® RNAiMAX
Lipofectamine® 2000
Lipofectamine® LTX
Neon® Electroporation
Invivofectamine® 2.0					N/A	N/A	In vivo

Primary Cells

Reagent	DNA	mRNA	RNAi	Co- delivery	Neurons	Stem Cells	Blood Cells	Others
NEW Lipofectamine® 3000
Lipofectamine® RNAiMAX
Lipofectamine® 2000
Lipofectamine® LTX
Neon® Electroporation
Invivofectamine® 2.0					N/A	N/A	In vivo

Additional Life Technologies™ transfection reagents

Transfection reagent	Key features & applications of reagent
ExpiFectamine® 293 Transfection Kit	Designed for transfection of high density suspension cell culture, with matching transfection enhancers that boost transfection performance and protein expression Achieves protein yields 2- to 10-fold higher than other transfection reagents used on high density 293 cell cultures Provides robust and reproducible transfection results Scale transfections for culture volumes of less than 1 mL to greater than 10 liters, while maintaining equivalent volumetric protein yields
FreeStyle® MAX Transfection Reagent	Optimized for transient transfection in CHO suspension cells and also works for HEK-293 cells Used for a large-scale transient protein production with milligrams of protein yield
Lipofectamine® 2000 CD Transfection Reagent	Same performance as Lipofectamine® 2000, certified animal-origin free (“CD” = chemically defined)
Oligofectamine® Transfection Reagent	Transfection of antisense oligonucleotides
293fectin™ Transfection Reagent	Used for transient protein production in combination with the FreeStyle™ 293 Expression System Optimized for suspension FreeStyle™ 293-F cells
Cellfectin® II Transfection Reagent	Optimal transfection of insect cells, including S2, Sf9, Sf21 and High Five™ cells
DMRIE-C Transfection Reagent	Transfection of suspension cells, including CHO, lymphoid and Jurkat cell lines.
Lipofectamine® Transfection Reagent	First generation reagent for plasmid DNA transfections In most cases, Lipofectamine® 2000 provides better performance than Lipofectamine® Transfection Reagent
Lipofectin® Transfection Reagent	First generation reagent for plasmid DNA transfections In most cases, Lipofectamine® 2000 provides better performance than Lipofectin® Transfection Reagent
Optifect™ Transfection Reagent	Broad use reagent designed for low confluency applications (<70% confluent at the time of transfection) Useful for cell lines that are sensitive to transfection reagents

General information