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Endpoint PCR (PCR) is a method used to determine if a gene of interest is expressed in a sample. Once cDNA is made through reverse transcription, it is amplified using a DNA polymerase-based reaction in a thermal cycler. Quality enzymes and precise temperature control improve the specificity and reproducibility of results. Select from a broad range of enzymes, for a solution to every challenge; and industry-renowned thermal cyclers that meet your lab needs. Thermo Fisher Scientific also offers a comprehensive portfolio of nucleic acid electrophoresis products, whether you prefer pre-cast or pour-you-own gels.
Our engineers have been designing and manufacturing high-quality thermal cyclers since 1987, earning a reputation for reliability, accuracy, and user-friendly interfaces. We offer a thermal cycler for every challenge, application, and budget.
ProFlex PCR System | NEWVeritiPro | SimpliAmp Thermal Cycler | MiniAmp Thermal Cycler | Automated Thermal Cycler (ATC) | |
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Block formats | 3 x 32-well 0.2 mL | 96-well 0.2 mL | 96-well 0.2 mL | 96-well 0.2 mL | 96-well 0.2 mL |
Small-scale experiments with a few samples | Daily experiment needs | Complete workflow experiment needs, ideal for automation |
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For gene expression assays, use hot-start PCR enzymes such as Invitrogen Platinum II Taq and Applied Biosystems AmpliTaq Gold DNA polymerases, both designed for enhanced target specificity.
Platinum II Taq | AmpliTaq Gold 360 DNA Polymerase | |
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Hot-start technology | Antibody | Chemical |
Enzyme activation time | 2 min | 10 min |
| Yes | No | |
DNA synthesis speed | 15 sec/kb | 60 sec/kb |
Flexible extension step* | Yes | No |
Inhibitor tolerance | Yes | No |
Amplification length | Up to 5 kb | Up to 5 kb |
GC-rich format | Yes | Yes |
Stand-alone enzyme | Colorless† | Colorless |
Master mix format | Colorless Green** | Colorless |
* The extension step can be extended up to 60 sec/kb without the effect of specificity.
** Contains electrophoresis tracking dyes and a density reagent for direct gel loading of PCR products.
† Green buffer available as separate item for use with stand-alone enzyme.
Instrumentation | Electrophoresis gels | Stains | Molecular weight markers |
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Learn about Invitrogen nucleic acid electrophoresis instruments for low to high throughput; and imagers to capture results. | Choose from pre-set E-gel cassettes, with a range of throughput and stain options, run on the E-Gel PowerSnap Device; or pour-your-own gel powders and reagents. | Find highly sensitive DNA staining reagents with formats include: SYBR Gold, SYBR Green I, SYBR Green II, and SYBR Safe dyes. | Choose your ladders from a wide variety of size ranges, applications, and formats – all exceeding industry standards. |
Style Sheet for Global Design System
Style Sheet for Komodo Tabs