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DNA Phosphoramidites |
Thermo Scientific DNA phosphoramidites are built to meet the varying needs of oligonucleotide manufacturers for the development of therapeutic, diagnostic, and research applications. We offer TheraPure, standard, and fast deprotect phosphoramidites.
Our DNA phosphoramidites are supported by:
†May not be applicable in all regions.
Processes are monitored from material procurement through final QC and product release for batch-to-batch reproducibility. Purity of each phosphoramidite lot is assessed by HPLC-MS, GC, 31P NMR, as well as Karl Fischer oven titration. Our rigorously controlled manufacturing processes help minimize levels of reactive P(III) impurities which could be incorporated into the oligonucleotide during synthesis and result in the accumulation of by-products.
Applied Biosystems/AKTA Synthesizer | Expedite Synthesizer | MerMade Synthesizer | ||
---|---|---|---|---|
TheraPure phosphoramidites | Bz-dA | ✓ | ✓ | ✓ |
Bz-dC | ✓ | ✓ | ✓ | |
iBu-dG | ✓ | ✓ | ✓ | |
dT | ✓ | ✓ | ✓ | |
Standard phosphoramidites | Bz-dA | ✓ | ✓ | ✓ |
Bz-dC | ✓ | ✓ | ✓ | |
iBu-dG | ✓ | ✓ | ✓ | |
dT | ✓ | ✓ | ✓ | |
dU | ✓ | ✓ | ||
dI | ✓ | |||
Fast deprotection (mild) | PAC-dA | ✓ | ||
Ac-dC | ✓ | ✓ | ✓ | |
DMF-dG | ✓ | ✓ | ✓ | |
iPrPAC-dG | ✓ |
For items not listed above, flexible/bulk packaging, or custom services, please contact us.
TheraPure phosphoramidites are manufactured with high purity and tight control of critical impurity level for excellent batch-to-batch reproducibility.
Click images to enlarge
dA(Bz) CEP | dC(Bz) CEP | dG(iBu) CEP | dT CEP | |
---|---|---|---|---|
Cat. No. | 27-2030 | 27-2032 | 27-2034 | 27-2036 |
Formula | C47H52N7O7P | C46H52N5O8P | C44H54N7O8P | C40H49N4O8P |
Molecular weight | 857.95 g/mol | 833.93 g/mol | 839.92 g/mol | 744.82 g/mol |
CAS # | 98796-53-3 | 102212-98-6 | 93183-15-4 | 98796-51-1 |
HPLC purity | ≥99% | |||
31P NMR purity | ≥99% | |||
Solution clarity | Free from undissolved particulate at 0.2 M solution in ACN | |||
Appearance | White to pale yellow powder | |||
Residual solvent | ≤3.0% total % by weight | |||
Shipping/Storage condition | Ambient / –20°C | |||
Water content | ≤0.3% | |||
Material origin | Non-animal source (no TSE/BSE risk) |
Not finding the product you need? Please contact us or request custom services.
Traditional base protection methods are used to help ensure quality and reliability for routine oligonucleotide synthesis.
Click images to enlarge
dA(Bz) CEP | dC(Bz) CEP | dG(iBu) CEP | dT CEP | |
---|---|---|---|---|
Cat. No. | 27-1730 | 27-1732 | 27-1734 | 27-1736 |
Formula | C47H52N7O7P | C46H52N5O8P | C44H54N7O8P | C40H49N4O8P |
Molecular weight | 857.95 g/mol | 833.93 g/mol | 839.92 g/mol | 744.82 g/mol |
CAS # | 98796-53-3 | 102212-98-6 | 93183-15-4 | 98796-51-1 |
HPLC purity | ≥98.0% | |||
31P NMR purity | ≥98.0% | |||
Water content | ≤0.3% | |||
Shipping/Storage condition | Ambient / –20°C | |||
Solution clarity | Free from undissolved particulate at 0.2 M solution in ACN | |||
Material origin | Non-animal source (no TSE/BSE risk) |
Not finding the product you need? Please contact us or request custom services.
They are ideal for applications requiring mild deprotection to preserve the integrity of oligonucleotides containing sensitive dyes, labels, or other modifications that may be unstable with prolonged exposure to strong alkaline conditions. Fast deprotect phosphoramidites permit the removal of base protecting groups under mild or accelerated conditions without the need for special reagents.
Click images to enlarge
iPrPAC-dG CEP | PAC-dA CEP | Ac-dC CEP | DMF-dG CEP | IBU-dC CEP | |
---|---|---|---|---|---|
Cat. No. (TheraPure phosphoramidites) | N/A | N/A | 27-2027 | N/A | N/A |
Cat. No. (standard phosphoramidites) | 27-1726 | 27-1723 | 29-1727 | 27-1737 | 27-1725 |
Formula | C51H60N7O9P | C48H54N7O8P | C41H50N5O8P | C43H53N8O7P | C43H54N5O8P |
Molecular weight | 946.05 g/mol | 887.96 g/mol | 771.8 g/mol | 824.9 g/mol | 839.92g/mol |
CAS # | 150065-82-0 | 110543-74-3 | 154110-40-4 | 330628-04-1 | 110522-84-4 |
HPLC purity | (TheraPure phosphoramidites) | ≥98.0% | ≥98.0% | ≥98% | (≥99%) | ≥98.0% | ≥98.0% |
31P NMR purity | (TheraPure phosphoramidites) | ≥98.0% | ≥98.0% | ≥98% | (≥99%) | ≥98.0% | ≥98.0% |
Shipping/Storage condition | Ambient / –20°C | ||||
Material origin | Non-animal source (no TSE/BSE risk) |
Not finding the product you need? Please contact us or request custom services.
For Research Use Only. Not for use in diagnostic procedures.