Search Thermo Fisher Scientific
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The SOLiD® Library TaqMan® Quantitation Kit facilitates the quantitation of amplifiable template in a SOLiD® library for use in emulsion PCR (ePCR) on the SOLiD® System. Complete with TaqMan® qPCR mix custom-designed for SOLiD® library adaptors and an adapted SOLiD® library control*, the kit is a highly specific solution for determining the optimal amount of starting material needed to most effectively carry out ePCR.
* The SOLiD® library control included in the kit is recommended for use only as a qPCR control, and not as a sequence control.
The SOLiD® Library TaqMan® Quantitation Kit is designed to quantitate libraries accurately regardless of size and can be used on any real-time instrument, using either fast or standard cycling programs. The kit has been validated for the quantitation of SOLiD® fragment libraries, SOLiD® short and long mate-paired libraries, SOLiD® whole transcriptome libraries, SOLiD® small RNA libraries, SOLiD® Chip-seq libraries, and SOLiD® SAGE™ libraries.
The multicolored amplification curves represent six SOLiD® Libraries quantitated by the SOLiD® Library TaqMan® Quantitation Kit (Cat. No. 4449639) on the Applied Biosystems® StepOnePlus™ Real-Time PCR System with fast cycling. Using a 5-point serial dilution from 100 pM to 10 fM of the SOLiD® Library qPCR Standard, the SOLiD® library input can accurately be measured. The gray line represents a no-template control.
For Research Use Only. Not for use in diagnostic procedures.