Reprogramming somatic cells to induced pluripotent stem cells is a critical and potentially time intensive step in your stem cell research. That’s why Life Technologies offers choices in integration-free reprogramming technologies and services that fit your research and resource needs – you can do it yourself or let us do it for you.

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Now available:Essential 6™ Medium for feeder-free reprogramming

Which reprogramming product or service is right for you?

Episomal iPSC Reprogramming Vectors*Epi5™ Episomal iPSC Reprogramming Kit**CytoTune®-iPS Sendai Reprogramming KitCytoTune®-iPS 2.0 Sendai Reprogramming Kit
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DescriptionProven technology for viral-free iPSC generation High efficiency viral-free system requiring no small moleculesEfficient generation of integration-free iPSCs from multiple somatic cell types Highest efficiency integration-free reprogramming system
Reprogramming Efficiency 0.002–0.08%0.04–0.3%0.01-0.5%0.02 – 1.2%
Genes UtilizedThomson/Yamanaka factorsYamanaka factors +Lin28Yamanaka factorsYamanaka factors
Method & Medium for Reprogramming FibroblastsElectroporation using Neon® in N2/B-27 Medium + small moleculesElectroporation using Neon® in N2/B-27 MediumAdd CytoTune® to cells in fibroblast mediumAdd CytoTune® 2.0 to cells in fibroblast medium
iPSC Growth MediumDay 15: Transfer to Essential 8™ MediumDay 15: Transfer to Essential 8™ Medium Day 7: Transfer to Essential 8™ Medium or KnockOut™ Serum Replacement supplemented medium Day 7: Transfer to Essential 8™ Medium or KnockOut™ Serum Replacement supplemented medium
Genomic Integration-free ✓✓✓✓
Virus-free Reprogramming ✓✓
Blood Cell Reprogramming ✓✓✓✓
Transfection Products Neon® Transfection System or similar deviceNeon® Transfection System or Lipofectamine® 3000None None
Available in CellModel™ Services✓✓✓
Viral Clearance N/AN/AUp to 15 passagesUp to 5 passages
Reporter Available N/AN/ACytoTune®-EmGFP Sendai Fluorescence ReporterCytoTune®-EmGFP Sendai Fluorescence Reporter
*Commercialized in Partnership with Cellular Dynamics International.
**Designed by Dr. Okita in the laboratory of Professor Yamanaka at the Center for iPS Cell Research and Application (CiRA), Kyoto University