Gibco Freedom ExpiCHO-S Kit Frequently Asked Questions

Volumetric titers of from 3 g/L in fed batch have been obtained with the Freedom ExpiCHO-S Kit.

It is possible to go from transfection to lead clone in as little as 4 months with the Freedom ExpiCHO-S Kit.

After execution and payment for a Commercial Production License, a Cell Line Documentation Package (>200 pages, and specific to the lot of cells purchased), is provided, which includes the entire lineage history of the cells, starting at receipt of the initial vial at Life Technologies through delivery to the purchaser, as well as, all testing reports.

CHO-S clones producing a model IgG protein were assessed for stability by comparing volumetric productivity for over 60 generations. Four out of the five clones that were tested maintained expression levels over 60 generations. Expression levels were considered stable if the titer at the end of the stability assessment (at generation 60) was at least 70% of the titer observed at the beginning of the stability study.

Yes. Freedom ExpiCHO-S cells are intended (with the purchase of a commercial license) for the development of biologics. They have been produced in a cGMP animal origin-free environment and have been scaled up in a commercial setting to produce products for clinical testing.

The Freedom ExpiCHO-S kit contains all of the initial components needed for the development of stable clones, including the cGMP banked cells, vectors, and media.

A certificate of analysis (COA) demonstrating suitability for research use is available to everyone who purchases Freedom ExpiCHO-S cells. A full documentation package, including the cell line's lineage, history, and cGMP testing results, is offered upon execution of a commercial use license.

The Freedom ExpiCHO-S two vector system provides the ability to control the ratio of heavy to light chains while allowing for consistent genomic background for transient and stable workflows.

Puromycin in combination with methotrexate (MTX) is used to select for stable pools following transfection with the Freedom pCHOm 3.1 and Freedom pCHOp 3.2 vectors.

ExpiCHO Stable Production Media and Efficient-Pro Feed 2 are recommended for use with the Freedom ExpiCHO-S kit. and Efficient Feed Pro are recommended for use with the Freedom ExpiCHO-S kit.

Limiting dilution cloning is recommended with the Freedom ExpiCHO-S Kit.

FreeStyle MAX Reagent is the preferred transfection method. ExpiCHO-S cells are also compatible with the Neon Transfection System (optimization of instrument settings is required).

Only two transfections are recommended.

A commercial use license is required prior to the sale of expressed proteins, or when an IND submission is being prepared for human clinical studies.

No. There is a one-time license fee required prior to IND and an additional fee at time of commercialization.

Please contact our licensing team to learn more.

Yes. Our Field Application Scientists provide technical support.

Yes,we offer limited cell line development services. Please contact your local Thermo Fisher representative to learn more about our services and capabilities or request more information.