Cancer RNA Expression | Thermo Fisher Scientific

In the study of diseases like cancer, it's not enough to know whether a gene is mutated or normal—it's also important to know whether that gene is expressed and how it is expressed. Transcriptome analysis of cancer samples is of growing importance in understanding how the altered expression of genetic variants contributes to cancer and other diseases. Analysis of differential RNA expression provides researchers with greater insights into biological pathways and molecular mechanisms that regulate cell fate, development, and disease progression.

Methods used

Next-generation sequencing and advances in real-time PCR (qPCR) throughput have revolutionized the way differential gene expression is analyzed, including variability between gene alleles and differently spliced transcripts, non-coding RNAs, post-transcriptional mutations and editing, and gene fusions. Currently, DNA microarrays are the most widely used method to analyze global patterns of gene expression.

Discover, profile, and functionally analyze more mRNA and non-coding RNAs that regulate cancer with sequencing technologies that provide significant advantages over microarray and flexible real-time PCR assay formats for meeting high, medium, and low throughput needs.

Transcriptome Sequencing

Identify and quantify both known and novel transcripts with a theoretically unlimited dynamic range. Reveal the diversity and subtleties of the transcriptome with Ion RNA-Seq on Ion Proton Sequencer or the 5500xl SOLiD Sequencer.

Targeted RNA Sequencing

Determine relative changes in human RNA expression without the need to sequence the entire transcriptome with as little as 5ng of RNA isolated from formalin-fixed, paraffin-embedded (FFPE) samples.

Gene Expression Analysis by Real-Time PCR

Sensitively detect virtually any gene product in human, mouse, and rat with access to more than 1.3 million predesigned TaqMan Gene Expression Assays.

Small RNA and miRNA sequencing

Identify and analyze new miRNA and Small RNA expression profiles in cancer samples.

Hybridization-based Gene Expression Analysis

Innovative Affymetrix products that help advance the gene expression investigations of cancer researchers via microarray analysis, from discovery to clinical research and validation.

Cancer RNA expression webinar
Cancer RNA expression posters

Cancer RNA expression webinar

Learn how our solutions are being used to identify novel RNA expression signatures from across the human transcriptome and overcome many of the challenges of working with tumor tissues in the research setting.