ELISA Kits and ELISA Components

High-quality optimized Invitrogen ELISA kits can help you measure target-specific proteins with confidence, reliability, and consistency. A variety of ELISA kit formats are available which include complete, ready-to-use kits as well as preoptimized reagents to design your own assay. ELISA kits and antibody pairs are available for a range of different species including human, mouse, rat, non-human primate, canine, porcine, bovine, equine, and feline. In addition to the off-the-shelf ELISA formats, special services such as: lot reservation, bulk packaging, and custom target development are available for flexibility and convenience as you start your next research project.

Search ELISA targets  ELISA for endocrinology

	Coated ELISA kits*	Rapid ELISA kits	Instant ELISA kits	Uncoated ELISA kits	Matched antibody pair & PeproTech development kits
	Explore coated ELISA targets	Explore Rapid ELISA targets	Explore Instant ELISA targets	Explore uncoated ELISA targets	Explore matched antibody pair targets
Features
Overnight coating	Not required	Not required	Not required	Required	Required
Analytical sensitivity**	++	++	++	+	+
Sample volume	10–100 µL	50 µL	25–50 µL	10–100 µL	10–100 µL
Sample-to-result time	125–380 min (kit specific)	90 min	130–250 min (kit specific)	24 hrs	>24 hrs
Data analysis	Plate reader with curve-fitting software
	Learn more below about coated ELISA kits	Learn more below about Rapid ELISA kits	Learn more below about Instant ELISA kits	Learn more below about uncoated ELISA kits	Learn more about matched antibody pair kits

*Values in this table refer to our Standard Colorimetric kits. A select number of ultrasensitive kits are also available.
**Every assay has its own specifications. Please consult the protocol for your specific immunoassays/kits.
 

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Invitrogen coated ELISA kits are ready-to-use, and have been tested to provide very low inter- and intra-assay variability, with very high lot-to-lot consistency. ELISA kits with coated plates help ensure that you obtain consistent ELISA performance and are designed for labs that require validated and comprehensively tested kits.

Benefits of coated ELISA kits:

• Validated performance⁠—tested for plate variability (CV) and final kit validation testing before shipping out
• Easy-to-use⁠—includes all the required assay buffers and reagents, and a straightforward protocol
• Fast⁠—pre-coated plates allow for a workflow typically completed within four hours

Coated ELISA kit contents

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Invitrogen Rapid ELISA kits have a condensed workflow with only one wash step, reducing the overall assay time to 90 minutes. Compared with conventional ELISA that requires three incubations for samples, biotinylated antibodies and enzyme conjugates, Rapid ELISA kits can be completed in one step, reducing operating steps by 1–2 hours.

Benefits of Rapid ELISA kits

• Short assay time—only 90 min from sample to results
• Ease of use—simplified ELISA procedure with a single wash step
• Excellent performance—Comparable sensitivity and performance with conventional ELISA

Figure 1. Rapid ELISA kit run time compared to conventional ELISA.

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Invitrogen Instant ELISA Kits have a condensed workflow format designed to alleviate the inconveniences of multiple preparation and incubation steps. In conventional ELISA kits, the precoated plates provide only the capture antibody, to which the sample and detection reagents must be added sequentially. In contrast, Instant ELISA Kits come with ready-to-use plates that contain all necessary assay components—including capture antibody and lyophilized detection antibody, streptavidin-HRP, and sample diluent. These prepared plates greatly reduce pipetting time and enable a simple one-wash protocol. Furthermore, serially diluted, lyophilized protein standards are provided in additional strip wells, eliminating the need for plate setup and serial dilutions to create a standard curve, leaving less room for error.

Benefits of Instant ELISA kits

• Ready-to-use—microplates contain all necessary assay components
• Time saving—standards are already serially diluted in the plate
• Convenient—sequential addition of reagents during assay minimized

Figure 2. Comparison of Instant ELISA technology vs. conventional ELISA procedures. With Instant ELISA kits, all the preparation work has been done for you, helping decrease hands-on time per experiment. In addition, Instant ELISA kits come with a full 96-well plate for your samples and two separately packed standards. This enables the use of all 96 wells for your samples expanding your ELISA capacity by 20%.

Table 1. Comparing conventional ELISA and Instant ELISA workflow steps. Fewer steps help save time and reduce variation. There are only 7 working steps when using an Instant ELISA kit, as opposed to 17 steps when performing a conventional ELISA. Therefore, you can decrease total hands-on time per experiment to about 1 hour by adopting the Instant ELISA technique.

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ELISAs can be used to identify and quantitate just about any type of analyte. When designing an ELISA for your unique target of interest, deciding which components are right for you is often the most difficult part. The ELISA Builder is an online selection tool that guides you through a short series of questions about your specific ELISA and then recommends all the components you’ll need—from plates to stop solution.

Invitrogen uncoated kits are ideal for experienced ELISA users on a budget with less stringent requirements for inter- and intra- assay variance. These kits have the reagents needed to coat-your-own plates using an overnight process and run the assay. Kit configurations can provide easy-to-use, flexible options.

Benefits of uncoated ELISA kits

• Pre-titrated and optimized—matched antibody pairs and all provided reagents optimized for ELISA development
• Many options for kit configuration—purchase based on the volume of your assay through a choice of package sizes, including 2-, 10-, and 20-plate kits
• Complete—includes all the essential reagents and buffers to limit variability. Generic reagents like wash and stop solution not included
• Affordable—priced to accommodate demanding budgets and help maximize your research

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Invitrogen antibody pair kits contain pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own ELISA assays. PeproTech Development kits are also used to design new ELISA protocols, with a variety of targets available in both ABTS and TMB formats for flexibility.

Benefits of matched antibody pair kits

• Flexible—matched antibody pairs can be used for ELISA or with other immunoassay platforms
• Convenient packaging option—All-in-one buffer kit sold separately, if needed for ELISA development

Matched antibody pair kit contents

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Benefits of ELISA development kits

• Flexibility—available in both ABTS and TMB formats for design flexibility
• Convenience—specifically formulated Buffer Kits available

ELISA development kit contents

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