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Microbial Detection Using Real-Time PCR
As emerging infectious diseases and antimicrobial resistance shape the future of pharmaceutical and biotechnological development, molecular microbial detection has become commonplace in the workflow of various scientific processes. Microbial identification by real-time PCR (qPCR) offers a distinct advantage over culture-based methods, providing a highly specific means of detection without waiting on the growth of the organism. Contamination concerns in biopharmaceutical and biotechnology manufacturing processes highlight qPCR microbial identification assays as a solution for the microbial surveillance of various components of production.
Gene expression assay search tool
Choose predesigned assay
Amplify DNA
- TaqPath BactoPure Microbial Detection Master Mix—General Purpose Reagent (GPR) for DNA target(s)
- TaqPath 1-Step RT-qPCR Master Mix—General Purpose Reagent (GPR) for RNA target(s)
- TaqMan Fast Advanced Master Mix—Research Use Only (RUO) for DNA target(s)
- TaqMan Fast Virus 1-Step Master Mix—Research Use Only (RUO) for RNA target(s)
Run qPCR
Instruments for real time PCR and digital PCR
- QuantStudio 3 Real-Time PCR System—simple to use for all levels of experience
- QuantStudio 5 Real-Time PCR System—easy controls for optimized experiments
- QuantStudio 6 & 7 Pro real-time PCR systems—high versatility and intuitive design
- QuantStudio 12k Flex Real-Time PCR System—maximum throughput, flexibility, and scalability
Analyze for detection
Process and interpret qPCR data with intuitive software data analysis tools for a range of applications
Choose predesigned assay
Amplify DNA
- TaqPath BactoPure Microbial Detection Master Mix—General Purpose Reagent (GPR) for DNA target(s)
- TaqPath 1-Step RT-qPCR Master Mix—General Purpose Reagent (GPR) for RNA target(s)
- TaqMan Fast Advanced Master Mix—Research Use Only (RUO) for DNA target(s)
- TaqMan Fast Virus 1-Step Master Mix—Research Use Only (RUO) for RNA target(s)
Run qPCR
Instruments for real time PCR and digital PCR
- QuantStudio 3 Real-Time PCR System—simple to use for all levels of experience
- QuantStudio 5 Real-Time PCR System—easy controls for optimized experiments
- QuantStudio 6 & 7 Pro real-time PCR systems—high versatility and intuitive design
- QuantStudio 12k Flex Real-Time PCR System—maximum throughput, flexibility, and scalability
Analyze for detection
Process and interpret qPCR data with intuitive software data analysis tools for a range of applications
Common microbial identification and ABR assay applications
- Pathogen research
- Microbiome research
- Antibacterial resistance research
- Environmental testing
- Wastewater testing
- Drinking water testing
- Viral culture monitoring
- Cell culture monitoring
- Food/beverage testing
- Biotechnology quality control
- Biopharmaceutical quality control
All assays employ Applied Biosystems TaqMan probe-based chemistries to produce highly sensitive and specific results with broad multiplexing capability.
Multiplex qPCR with microbial assays
Multiplex PCR is an effective way to help reduce the costs of and improve the efficiency of your microbial identification assays. The relative costs for a multiplex reaction are much closer to those for a singleplex reaction and offer substantial savings when compared to the costs of performing four singleplex reactions independently.
All TaqMan microbial identification assays are available with both FAM and VIC dye configurations accommodating the easy design of your duplex reactions. For higher order multiplexing with up to four targets, TaqMan microbial identification assays are also compatible with ABY and JUN dye configurations through Specialty Oligos.
For details and information around how best to design your multiplex assay to accommodate the needs of your laboratory, please see Multiplex Real-Time qPCR Solution.
For Research Use Only. Not for use in diagnostic procedures.