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QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) is a sample preparation method that was initially developed in 2003 for analyzing veterinary drugs in food. The method was also tested for pesticide residue analysis in plant material with great success. In 2003, the detailed method was published in the journal AOAC International. Since then, the QuEChERS method has evolved into a sample preparation technique used on a variety of analytes in an expanding number of sample types before analysis by GC-MS or LC-MS.
Thermo Scientific QuEChERS kits contain pre-packaged, ready-weighed salts, sorbents, and buffers to streamline your workflow and minimize the potential for error. The kits are grouped according to your analytes of interest, sample matrix, and preferred QuEChERS method:
Complete packages—including both extraction and clean-up kits—are available. Extraction-only and cleanup-only kits are also offered to suit your laboratory’s sample preparation needs. These pre-packaged kits save time and money, make QuEChERS procedures easier and more convenient, and ensure quality to yield reproducible results and excellent recoveries for a wide variety of analytes.
As shown in the workflow diagram below, the QuEChERS method has four basic steps:
The original QuEChERS method (Anastassiades et al, 2003) was developed by Steven J. Lehotay and Michelangelo Anastassiades in 2003 for the determination of pesticide residues in fruits and vegetables. The original method was created to analyze non-base-sensitive compounds and uses a sodium chloride buffer to reduce polar interferences and enhance extraction. Buffering is an important part of the QuEChERS procedure because it allows for analysis of pesticides and other residues that display pH dependence in their recoveries, depending on the sample matrix. Three different extraction kits are available to support the original 2003 QuEChERS method.
Original 2003 method extraction kits | ||||||
---|---|---|---|---|---|---|
Part number | Extraction salts | Also includes | Starting sample | Unit size | Purchase | |
S1-10-ORIG-CH-KIT |
|
| 10 g sample | 50/PK | ||
S1-10-ORIG-KIT |
| 50 mL tubes | 10 g sample | 50/PK | ||
S1-10-ORIG-POT |
| ̶ | 10 g sample | 50/PK | ||
S1-15-ORIG-CH-KIT |
|
| 15 g sample | 50/PK | ||
S1-15-ORIG-KIT |
| 50 mL tubes | 15 g sample | 50/PK | ||
S1-15-ORIG-POT |
| - | 15 g sample | 50/PK |
Chemicals used in the original 2003 method | |
---|---|
Acetonitrile | Used to perform single-phase extraction (SPE) on a sample |
Anhydrous MgSO4 | Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step |
NaCl | Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes residual water from sample; reduces polar interference and enhances extraction |
PSA | Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dispersive solid phase extraction (dSPE) step |
The AOAC 2007.01 QuEChERS Method was developed by Steven J. Lehotay and his co-workers, who modified the original 2003 method to accommodate the use of acetate buffering at higher concentration for greater buffering strength. Buffering is an important part of the QuEChERS procedure because it allows for analysis of pesticides and other residues that display pH dependence in their recoveries, depending on the sample matrix. These extraction and clean-up products here support the AOAC 2007.01 method.
AOAC 2007.01 method extraction kits | ||||||
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Part number | Extraction salts | Also includes | Starting sample | Unit size | Purchase | |
S1-15-AOAC-CH-KIT |
|
|
|
| ||
S1-15-AOAC-KIT |
|
|
|
| ||
S1-15-AOAC-POT |
| ̶ |
|
|
AOAC 2007.01 method clean-up kits | |||
---|---|---|---|
Sample type | Prefilled 2 mL tubes (100/PK) | Prefilled 15 mL tubes (50/PK) | |
Clean-up | General fruits and vegetables | S2-2-GFV-AOAC-KIT
| S2-15-GFV-AOAC-KIT
|
Pigmented fruits and vegetables | S2-2-P-AOAC-KIT
| S2-15-P-AOAC-KIT
| |
Fruits and vegetables with fats and waxes | S2-2-FW-AOAC-KIT
| S2-15-FW-AOAC-KIT
| |
Fruits and vegetables with pigments and fats | S2-2-PF-AOAC-KIT
| S2-15-PF-AOAC-KIT
| |
All food types | S2-2-ALL-AOAC-KIT
| S2-15-ALL-AOAC-KIT
| |
Other foods | S2-2-OTH-AOAC-KIT
| S2-15-OTH-AOAC-KIT
|
Acetonitrile | Used to perform single-phase extraction on a sample |
Anhydrous MgSO4 | Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step |
NaOAc | Buffers the sample to stabilize pH |
PSA | Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dispersive solid phase extraction (dSPE) step
|
C18 | Removes fats, sterols, and other non-polar interferences from sample |
GCB | Graphitized carbon black, removes pigment (not recommended for use with planar pesticides) |
The extraction and clean-up products here support the EN 15662 method. Widely used in Europe, the EN 15662 method was developed by Michelangelo Anastassiades and co-workers, who modified the original 2003 method to accommodate the use of citrate buffering at a relatively low buffering capacity. This variation uses a sodium chloride buffer to minimize polar interferences in addition to sodium citrate dihydrate and disodium citrate sesquihydrate buffers. Buffering is an important part of the QuEChERS procedure because it allows for analysis of pesticides and other residues that display pH dependence in their recoveries, depending on the sample matrix.
EN 15662 method extraction kits | ||||||
---|---|---|---|---|---|---|
Part number | Extraction salts | Also includes | Starting sample | Unit size | Purchase | |
S1-10-EN-CH-KIT |
|
| 10 g | 50/PK | ||
S1-10-EN-KIT |
| 50 mL tubes | 10 g | 50/PK | ||
S1-10-EN-POT |
| - | 10 g | 50/PK |
EN 15662 method clean-up kits | |||
---|---|---|---|
Sample type | Prefilled 2 mL tubes (100/PK) | Prefilled 15 mL tubes (50/PK) | |
| General fruits and vegetables
| S2-2-GFV-EN-KIT
| S2-15-GFV-EN-KIT
|
Pigmented fruits and vegetables | S2-2-P-EN-KIT
| S2-15-P-EN-KIT
| |
Highly pigmented fruits and vegetables
| S2-2-HP-EN-KIT
| S2-15-HP-EN-KIT
| |
Fruits and vegetables with fats and waxes
| S2-2-FW-EN-KIT
| S2-15-FW-EN-KIT
|
Chemicals used in the EN 15662 method | |
---|---|
Acetonitrile | Used to perform single-phase extraction on a sample |
Anhydrous MgSO4 | Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step |
NaCl | Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes residual water from sample |
Trisodium Citrate | Buffers the sample to stabilize pH |
Disodium Citrate Sesquihydrate | Buffers the sample to stabilize pH |
PSA | Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dispersive solid phase extraction (dSPE) step |
C18 | Removes fats, sterols, and other non-polar interferences from sample |
GCB | Graphitized carbon black, removes pigment (not recommended for use with planar pesticides) |
The NY/T 1380-2007 method is recommended by China Ministry of Agriculture for the QuEChERS Method sample preparation of 51 specific pesticide residues in fruits and vegetables with analysis by GC-MS.
NY/T 1380-2007 method clean-up kit | |||
Sample | Prefilled 15 mL tubes (50/PK) | Purchase | |
---|---|---|---|
Fruits and vegetables | S2-15-GFV-NYT-KIT
|
Chemicals used in the NY/T 1380-2007 method | |
---|---|
Anhydrous MgSO4 | Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step |
PSA | Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dSPE step |
C18 | Removes fats, sterols, and other non-polar interferences from sample |
LC-MS solution using start-to-finish workflows for quantitation and pesticide residue screening of food
LC-MS solution for routine screening or sensitive quantitation of veterinary drugs and antibiotics in food
Automated, high-throughput workflows for GC and LC automates QuEChERS clean-up of extracts
Part number | Product description |
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Vials & caps | |
6ASV9-1P | 2mL clear screw glass vial (9mm short thread) with write-on patch |
6ESV9-1PP | 1.5mL clear screw PP vial (9mm short thread) |
6ASV9-2P | 2mL amber screw glass vial (9mm short thread) with write-on patch |
6ASC9ST1R | Red screw cap (9mm) (PP: white silicone/red PTFE septa: 1.0mm thickness) with AVCS technology |
6ASC9ST1 | Blue screw cap (9 mm) (PP: white silicone/blue PTFE septa: 1.0 mm thickness) with AVCS technology |
Part number | Product description |
---|---|
Solvents | |
A956-1 | Thermo Scientific UHPLC-MS grade acetonitrile suitable for LC and GC |
A458-1 | Thermo Scientific UHPLC-MS grade methanol suitable for LC and GC |
W8-1 | Thermo Scientific UHPLC-MS grade water suitable for LC and GC |
A117 | Fisher Chemical Optima LC-MS grade formic acid suitable for LC and GC |
A115-50 | Fisher Chemical Optima LC-MS grade ammonium formate suitable for LC and GC |
T00101400 | Thermo Scientific Chromplete acetonitrile suitable for HPLC and GC |
D1514 | Optima Methylene Chloride for suitable HPLC and GC-MS |
H3064 | Optima 95% n-Hexane suitable for HPLC and GC-MS |
S415500 | Sodium sulfate anhydrous suitable for LC and GC |
Part number | Product description |
---|---|
HPLC columns | |
17326-102130 | Thermo Scientific Accucore aQ (100 × 2.1 mm, 2.6 μm) |
071387 | Thermo Scientific Acclaim Trinity P1 (anionic pesticides) (100 × 3.0 mm, 3.0 µm) |
17626-102130 | Thermo Scientific Accucore RP-MS (100 × 2.1 mm, 2.6 μm) |
17326-102130 | Thermo Scientific Accucore aQ (100 × 2.1 mm, 2.6 μm) |
Part number | Product description |
---|---|
GC consumables | |
453T2120 | Thermo Scientific LinerGold: Siltek six baffle PTV liner |
453A1925UI | SSL splitless liner, single taper, deactivated |
26096-1420 | Thermo Scientific TraceGOLD TG-5SIL MS (30 m x 0.25 mm i.d. x 0.25 μm) |