Lipofectamine RNAiMAX Transfection Reagent—In Vitro Delivery of siRNA

When it comes to achieving effective gene knockdown, Lipofectamine RNAiMAX Transfection Reagent outperforms other siRNA transfection reagents. High knockdown levels of target genes can be achieved with as little as 1 nM siRNA (Figure 1). The Lipofectamine RNAiMAX protocol recommends using 10 nM siRNA as a starting point to maximize knockdown levels. Even when coupled with low concentrations of siRNA, RNAiMAX reagent helps obtain high knockdown levels while is also beneficial in minimizing background expression from untransfected cells and to minimize other non-specific effects.

Transfection-mediated cytotoxicity can mask the true phenotype of a target gene being studied, so minimizing the amount of reagent used in your transfections is a critical factor for successful RNAi experiments.

Lipofectamine RNAiMAX Transfection Reagent gives maximal knockdown and excellent cell viability across a 10-fold concentration range of the reagent (Figure 2). This broad peak of optimal transfection activity with minimal cytotoxicity means you can be assured of achieving high knockdown levels despite differences in cell density, minor experimental inaccuracies, and other variations. Not also does this make it easy to optimize Lipofectamine RNAiMAX reagent for the lowest siRNA concentration but also reduces cellular stress and cytotoxicity in your experimental system simultaneously.

Lipofectamine RNAiMAX offers a simple protocol to achieve high transfection efficiencies in many cell types. There is no need to remove Lipofectamine-siRNA complexes, or change or add cell culture media following transfection.

When combined with Invitrogen BLOCK-iT Alexa Fluor Red Fluorescent Control, Lipofectamine RNAiMAX reagent gives you the most versatile approach to all your knockdown experiments (Table 1, Figure 3). Spend less hands-on time and get better results faster.