Ion Chef System | Thermo Fisher Scientific

Simple, reproducible automated sequencing

The Ion Torrent Ion Chef System simplifies the workflow for Ion GeneStudio S5 Series, Ion PGM, and Ion Proton systems, providing an automated, walk-away workflow solution for Ion AmpliSeq library preparation and reproducible template preparation and chip loading.

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Specification sheet | Learn more about Ion Torrent sequencing systems

Simplified workflow helps save time and labor

Intuitive instrument set-up for all automated workflows
Increased lab productivity—just 15 minutes of set-up time
Flexible Ion 550 Chef Workflow—1 or 2 loaded chips per run, with no loss of reagents

Reduced run variability

Reduced user intervention enables reproducible chip loading for users of any experience level
Sample and reagent tracking with onboard tracking system

Ion AmpliSeq library preparation automation

Expanded Ion Chef System capabilities enable automation of Ion AmpliSeq library generation
Accommodates one- or two-pool Ion AmpliSeq research panels for DNA analysis

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Ion Chef System automated workflow

The Ion Chef System automated workflow includes less than 45 minutes of hands-on time. The Ion Chef System fully automates library preparation, template preparation, and produces sequencing-ready Ion Torrent semiconductor chips.

Step 1Automated library preparation
Step 2Automated template preparation and chip loading
Step 3Sequencing

Ion AmpliSeq library preparation for up to 8 samples

Library amplification
ISP recovery and enrichment
Chip loading (up to 2 chips)

Sequence using:

Ion GeneStudio S5 Series
Ion PGM System
Ion Proton System

Step 1 : Automated library preparation

Ion AmpliSeq library preparation for up to 8 samples

Step 2 : Automated template preparation and chip loading

Library amplification
ISP recovery and enrichment
Chip loading (up to 2 chips)

Step 3 : Sequencing

Sequence using:

Ion GeneStudio S5 Series
Ion PGM System
Ion Proton System

Automated template preparation & chip loading with the Ion Chef System

The Ion Chef System simplifies the Ion Torrent next-generation sequencing workflow by integrating automated template preparation and chip loading in a single instrument for maximum efficiency and throughput. Simply select the run parameters using the intuitive touch screen interface, load the library samples, reagents, and consumables, and go.

Simplifying Next-Gen Sequencing Template Prep with Ion Chef

Ion Chef enables walk-away freedom

The Ion Chef System supports:

Instrument	Ion chip	Read length	Key research applications
Ion GeneStudio S5 Series	Ion 510 Chip	Up to 400 bp	Targeted DNA sequencing, targeted RNA sequencing, small RNA sequencing, de novo microbial sequencing, bacterial typing, viral typing, metagenomics, sequencing by genotyping
	Ion 520 Chip
	Ion 530 Chip
	Ion 540 Chip	Up to 200 bp	Exome sequencing, transcriptome sequencing, copy number analysis
	Ion 550 Chip
Ion PGM System	Ion 314 Chip	Up to 400 bp	Targeted DNA sequencing, targeted RNA sequencing, small RNA sequencing, de novo microbial sequencing, bacterial typing, viral typing, metagenomics, sequencing by genotyping
	Ion 316 Chip
	Ion 318 Chip
Ion Proton System	Ion PI Chip	Up to 200 bp	Exome sequencing, transcriptome sequencing, copy number analysis