Sanger Sequencing Software Support – Troubleshooting

For exporting and backing up the data, there are many ways to do so:

Export: takes the Project, Analysis Parameters, and Reference Data of your choice and saves them to a location of your choice. You can use this option for backing up data or sending data to support personnel. Exported files will not be removed from the Dashboard unless you delete them.

Archive: for Projects only: will take a selected Project and export it to a location of your choice. The Project is then removed from the Project Dashboard. You cannot archive Analysis Parameters or Reference Data.

Back-up: will duplicate all of the information in the Data Store (Projects, Analysis Parameters, and Reference Data) to a folder of your choice. This is an all-or-nothing option—you cannot select specific files for back-up. If you wish to have specific files backed up, please use the Export option.

Exporting Projects, Analysis Parameters, and Reference Data
More information on exporting data can be found in the Variant Reporter Help Contents under the keyword “Export”.

To export a Project, Analysis Parameter, or Reference from the Dashboard:

1. Open the Dashboard View (see below), then click on the Tab (Projects, Analysis Parameters, or References) where the file you want to export is located and left-click on the file name to select it.
2. Click  to open the Export File dialog box.
3. Select the location you want to save the file in, then click Save.

To back up the Data Store:

1. Open the Dashboard View.
2. Click .
3. In the Browse for Back-up Folder Destination dialog box, select the folder that you want to back up the Data Store to, then click OK. Your project will start to get backed up.

Note: All associated Projects, Analysis Parameters, and References contained in the current Data Store will be copied to the folder you selected when you back up the Variant Reporter Data Store.

For importing data, there are many ways to do so:

More information on backing up the Data Store can be found in the Variant Reporter Help Contents under the keyword “Back-up”.

1. Select the Data Store where you would like the data imported to. To choose a Data Store:
   1. Click the Manage button in the Data Store section of the Dashboard View.

2. Highlight the Data Store you wish to use and click on Change Location to select it. Click Close to close the window. 
   

"Out of memory" issues may occur during analysis when library sequence lengths exceed 2,300 bases with 6,000 entries. When creating any custom library, we recommend creating ~500 bp sequence length for a 500 kit library, ~1,500 bp sequence length for a full gene kit, and ~350 bp sequence length for a fungal kit.

"Out of memory" issues may occur when exporting reports in PDF format for large projects (~50 Specimen Full Gene project) analyzed with library sequence lengths exceeding 2,300 bases with 6,000 entries.

Archive the audit records and delete them from the current report. They can be restored back to the report if needed.

We recommend that you exit and reopen the MicroSEQ ID Software after running for a long period of time.

Backing up data can help protect you from data loss and/or for sending data to Technical Support for troubleshooting.
To export Projects, Project Templates, Reference Data Groups, Libraries, Analysis Defaults, Analysis Protocols, and Display Settings:

1. Go to the Tools menu and select SeqScape Manager.
2. Click on the tab that contains the file(s) you want to back up or export.

3. Select the file(s) that you want to export. Hold down the Shift key while selecting files to select a group of files, or the Control key to select multiple individual files. Once they are selected, click on Export at the bottom of the window.

4. Browse to the location where you want to save the files, and click Export to save them (Note: When selecting multiple files, the File name will appear as the pathway to the location where you are going to save the files.)
   

If you are performing a data backup, you will want to repeat steps 2–4 for each tab to make sure that all of your work is backed up. Once you have backed them up, save the files to the storage media of your choice (CD, DVD, network drive, flash drive).

Please see the table below outlining the different types of files, exported file type, and data contained in the file:

If you are upgrading or reinstalling software, you may want to import your data. To do so:

1. Go to the Tools menu and select SeqScape Manager.
2. Click on the tab where you want to import the data (e.g., if you want to import a project, click on the Projects tab).

3. Click on the Import button and select the files you wish to import. Then click Import.
   

After clicking on Import, a progress bar will appear on the screen. If the file is imported successfully, it will appear in the list for that tab. If the file is not, an error message will appear explaining why the file was not imported. In the example below, the Project name already existed in the database.