Thermo Scientific EnzymeRecommended buffer for
100% activity
Enzyme activity in 5-buffer system buffers, %Tango buffer for double digestionUnits for overnight incubation,
u/μg DNA
Thermal inactivation in 20 min
B
(blue)
1X
G
(green)
1X
O
(orange)
1X
R
(red)
1X
Tango
(yellow)
1X / 2X
AanI (PsiI)Tango50-10050-1000-200-2010020-501X or 2X0.565°C
AarIAarI +oligoNR (+oligo)NR (+oligo)0-20 (+oligo)0-20 (+oligo)NR (+oligo)50-100 (+oligo)2X (+oligo)0.965°C
AatIITango50-10020-500-200-2010020-501X or 2X0.365°C
Acc65I (Asp718I)O0-2020-5010020-5020-5050-1001X or 2X0.365°C
AdeI (DraIII)G0-2010020-50100100*20-501X* or 2X0.5No
AjiI (BmgBI)AjiINRNR20-50*NRNR20-50*2X*0.565°C
AjuIR +SAM0-20 (+SAM)50-100 (+SAM)20-50 (+SAM)100 (+SAM)50-100 (+SAM)50-100 (+SAM)1X or 2X (+SAM)0.565°C
AloIR0-200-200-2010020-501001X or 2X0.165°C
AluITango50-1000-200-200-2010020-501X or 2X0.165°C
Alw21I (BsiHKAI)O0-2020-5010050-10020-5050-1001X or 2X0.165°C
Alw26ITango50-1001000-200-201001001X or 2X0.265°C
Alw44ITango50-1001000-2050-10010050-1001X or 2X0.165°C
ApaIB10020-500-200-2020-500-201X0.265°C
BamHIBamHI20-50*10020-5050-100*100*50-1001X* or 2X0.580°C (10u)
BauI (BssSI)Tango0-2050-1000-2050-10010050-1001X or 2X0.265°C
BclIG20-5010020-5020-50100*1001X* or 2X0.180°C (10u)
BcnI (NciI)Tango20-5050-10050-10050-10010050-1001X or 2X0.265°C
BcuI (SpeI)Tango50-10050-1000-2020-501000-201X0.5No
BfmI (SfcI)Tango0-2050-1000-200-2010020-501X or 2X0.965°C
BfuI (BciVI)BfuINRNR0-200-20NR50-100*2X*0.980°C
BglIO0-2050-1001001000-201002X0.165°C
BglIIO0-2020-5010050-1000-201002X0.1No
Bme1390I (ScrFI)O20-5050-10010050-10050-10050-1001X or 2X0.280°C
BoxI (PshAI)Tango0-200-200-2020-5010020-501X or 2X0.580°C
BpiI (BbsI)G20-5010050-10050-10050-10050-1001X or 2X0.365°C
BplITango0-20 (+SAM)20-50 (+SAM)0-20 (+SAM)0-20 (+SAM)100 (+SAM)20-50 (+SAM)1X (+SAM)0.365°C
Bpu10IBpu10I0-2020-50*50-100*100*50-100*100*1X* or 2X*0.280°C
Bpu1102I (BlpI)Tango50-10050-10020-5020-5010020-501X or 2X0.180°C
BseDI (BsaJI)Tango50-10020-500-200-2010050-1001X or 2X0.280°C
BseGI (BtsCI)Tango20-5050-10020-5020-5010020-501X or 2X0.280°C
BseJI (BsaBI)ONR100*100NRNR100*2X*0.1No
BseLI (BslI)Tango20-501000-2020-5010050-1001X or 2X0.1No
BseMI (BsrDI)R0-2020-500-2010050-10050-1001X or 2X0.380°C
BseMII (BspCNI)Tango50-100 (+SAM)50-100 (+SAM)50-100 (+SAM)50-100 (+SAM)100 (+SAM)50-100 (+SAM)1X or 2X (+SAM)0.580°C
BseNI (BsrI)B10020-500-200-2050-10020-501X or 2X0.180°C
BseSI (Bme1580I)G20-501000-2020-5050-1000-201X0.180°C (10u)
BseXI (BbvI)BseXINRNRNRNRNRNRNR0.380°C
Bsh1236I (BstUI)R0-200-2050-10010020-5050-1001X or 2X0.165°C
Bsh1285I (BsiEI)G20-5010020-500-200-2020-502X0.265°C
BshNI (BanI)O0-2020-5010050-1000-201002X0.365°C
BshTI (AgeI)O0-2020-5010050-10020-5020-501X or 2X0.165°C
Bsp119I (BstBI)Tango20-500-200-200-201001001X or 2X0.180°C
Bsp120I (PspOMI)B10020-500-2020-5050-1000-201X0.180°C
Bsp1407I (BsrGI)Tango0-2020-500-2020-5010050-1001X or 2X0.165°C
Bsp143I (Sau3AI)Bsp143I20-5020-500-200-2050-10020-501X or 2X0.165°C
Bsp68I (NruI)O0-2020-5010050-10020-5050-1001X or 2X0.165°C
BspLI (NlaIV)Tango50-10050-1000-2020-5010020-501X or 2X0.365°C
BspOI (BmtI)O0-200-201001000-2020-502X0.280°C
BspPI (AlwI)Tango20-5020-500-200-201000-201X0.580°C
BspTI (AflII)O0-200-2010020-500-2050-1002X0.165°C
Bst1107I (BstZ17I)O20-5050-10010010020-501001X or 2X0.165°C
BstXIO20-5010010050-10050-1001001X or 2X0.165°C
Bsu15I (ClaI)Tango20-5020-5020-5020-5010020-501X or 2X0.165°C
BsuRI (HaeIII)R0-200-200-2010050-1001001X or 2X0.180°C
BveI (BspMI)O +oligo0-20 (+oligo)20-50 (+oligo)100 (+oligo)20-50 (+oligo)50-100 (+oligo)100 (+oligo)1X or 2X (+oligo)0.265°C
CaiI (AlwNI)Tango20-5020-5020-5050-10010050-1001X or 2X0.265°C
Cfr10I (BsrFI)Cfr10I0-2020-5020-5050-10020-5050-1001X or 2X0.1No
Cfr13I (Sau96I)Tango50-10050-10020-5020-5010020-501X or 2X0.365°C
Cfr42I (SacII)B10050-1000-200-2050-1000-201X0.165°C
Cfr9I (XmaI)Cfr9I0-200-200-200-2020-500-201X0.265°C
CpoI (RsrII)Tango20-5050-10050-10020-5010050-1001X or 2X0.565°C
CseI (HgaI)RNR50-100*50-100100100*50-1001X* or 2X0.580°C (10u)
Csp6I (CviQI)B10050-1000-200-2050-1000-201X0.165°C
DpnITango10010050-10050-10010050-1001X or 2X0.180°C
DraITango50-10050-10020-5020-5010050-1001X or 2X0.165°C
Eam1104I (EarI)Tango50-10050-1000-200-201000-201X0.565°C
Eam1105I (AhdI)Eam1105I20-5050-1000-200-2050-10020-501X or 2X0.165°C
Ecl136II (EcoICRI)Ecl136II50-10020-500-200-2050-1000-201X0.265°C
Eco105I (SnaBI)Tango100*50-1000-200-201000-201X0.565°C
Eco130I (StyI)O0-2020-5010050-10050-1001001X or 2X0.265°C
Eco147I (StuI)B10050-10020-5020-5050-1000-201X0.180°C
Eco24I (BanII)Tango50-10050-10020-5020-501000-201X0.265°C
Eco31I (BsaI)G50-1001000-200-2050-10020-501X or 2X0.365°C
Eco32I (EcoRV)R0-2050-10050-10010020-501001X or 2X0.165°C
Eco47I (AvaII)R0-2050-10050-10010050-10050-1001X or 2X0.365°C
Eco47III (AfeI)O0-2020-5010010050-1001001X or 2X0.180°C
Eco52I (EagI)Eco52I0-200-200-2020-500-2020-502X0.265°C
Eco57I (AcuI)G +SAM100 (+SAM)100 (+SAM)20-50 (+SAM)20-50 (+SAM)50-100 (+SAM)50-100 (+SAM)1X or 2X (+SAM)0.965°C
Eco72I (PmlI)TangoNRNR0-200-2010020-501X or 2X0.565°C
Eco81I (Bsu36I)Tango50-1001000-200-201000-201X0.180°C
Eco88I (AvaI)Tango10050-1000-200-2010020-501X or 2X0.265°C
Eco91I (BstEII)O20-5020-5010050-100NR1002X0.165°C
EcoO109I (DraII)Tango50-10020-5020-5020-501001001X or 2X0.265°C
EcoRIEcoRI0-20NR100100*NR1002X0.265°C
EcoRIIO20-5050-10010050-10020-5050-1001X or 2X0.180°C
EheI (SfoI)Tango20-5050-1000-200-2010020-501X or 2X0.965°C
Esp3I (BsmBI)Tango100 (+DTT)20-50 (+DTT)0-20 (+DTT)0-20 (+DTT)100 (+DDT)0-20 (+DTT)1X (+DDT)0.265°C
FaqI (BsmFI)Tango20-50 (+SAM)20-50 (+SAM)0-20 (+SAM)0-20 (+SAM)100 (+SAM)50-100 (+SAM)1X (+SAM)0.980°C
FspAIO0-200-2010050-1000-2050-1002X0.265°C
FspBI (BfaI)Tango50-10020-500-200-201000-201X0.365°C
GsuI (BpmI)B10050-1000-2020-5010050-1001X or 2X0.965°C
HhaITango50-10050-10020-5020-5010020-501X or 2X0.180°C
Hin1I (BsaHI)G20-5010020-5020-5020-5020-501X or 2X0.165°C
Hin1II (NlaIII)G50-10010020-5050-10050-10050-1001X or 2X0.365°C
Hin6I (HinP1I)Tango50-10050-10020-5020-5010050-1001X or 2X0.165°C
HincII (HindII)Tango50-10050-10020-5050-10010050-1001X or 2X0.165°C
HindIIIR0-2020-500-2010050-10050-1001X or 2X0.180°C
HinfIR0-2020-5050-10010050-10050-1001X or 2X0.165°C
HpaIITango50-10050-1000-2020-5010020-501X or 2X0.165°C
HphIB1000-200-200-2020-500-201X0.165°C
Hpy8I (MjaIV)Tango50-10050-1000-2020-5010050-1001X or 2X0.180°C
HpyF10VI (MwoI)Tango0-200-200-200-2010050-1001X or 2X0.180°C
HpyF3I (DdeI)Tango20-5020-5020-5020-5010050-1001X or 2X0.265°C
I-SceITango50-10050-10050-10050-10010050-1001X or 2X0.965°C
Kpn2I (BspEI)Tango50-10050-1000-2020-5010050-1001X or 2X0.180°C
KpnIKpnI20-500-200-200-2020-500-201X0.280°C
KspAI (HpaI)B10050-100*20-5020-50100*50-1001X* or 2X*0.565°C
LguI (SapI)Tango20-5050-10020-5020-5010020-501X or 2X0.565°C
Lsp1109I (BbvI)Lsp1109I0-2020-50*50-100*10020-50*20-50*1X* or 2X0.565°C
LweI (SfaNI)Tango0-200-200-2020-5010020-501X or 2X0.265°C
MauBITango0-200-200-200-201000-201X0.165°C
MbiI (BsrBI)Tango20-501000-2020-5010020-501X or 2X0.265°C
MboIR50-10050-10050-10010050-1001001X or 2X0.165°C
MboIIB10050-10020-500-2050-10020-501X or 2X0.965°C
MlsI (MscI)R0-2020-500-2010020-5050-1001X or 2X0.565°C
MluIR0-2020-5050-10010020-5050-1001X or 2X0.165°C
MnlIG50-10010020-5020-5020-5020-501X or 2X0.565°C
Mph1103I (NsiI)R0-2050-10020-5010050-10050-1001X or 2X0.365°C
MreI (Sse232I)G0-201000-200-2050-1000-201X0.580°C
MspI (HpaII)Tango50-10050-1000-200-2010050-1001X or 2X0.365°C
MssI (PmeI)B1000-200-200-2020-500-201X0.565°C
MunI (MfeI)G1001000-200-201000-201X0.165°C
Mva1269I (BsmI)R0-2020-5050-1001000-2050-1002X0.165°C
MvaI (BstNI)R20-5020-5050-10010020-50*1001X* or 2X0.180°C (10u)
Nb.Bpu10IR0-2020-5020-5010020-5050-1001X or 2X0.380°C
Nb.Mva1269IO0-2020-5010010020-5050-1001X or 2X0.380°C
NcoITango20-5020-5020-5050-1001001001X or 2X0.165°C
NdeIO0-200-2010050-1000-2050-1002X0.365°C
NheITango10020-500-200-201000-201X0.265°C
NmuCI (Tsp45I)R0-2020-5050-10010020-5050-1001X or 2X0.165°C
NotIO0-2020-5010020-500-2020-502X0.165°C
NsbI (FspI)Tango20-5050-1000-2020-5010020-501X or 2X0.165°C
Nt.Bpu10IR0-200-201001000-2050-1002X0.380°C
OliI (AleI)R0-200-200-201000-2050-1002X0.265°C
PacIPacI20-5020-500-200-200-200-20NR0.365°C
PaeI (SphI)B10050-1000-200-2050-1000-201X0.165°C
PagI (BspHI)O0-2050-100100NRNRNRNR0.280°C
PasIPasINRNRNRNRNRNRNR0.280°C
PauI (BssHII)R0-200-201001000-201002X0.280°C
PdiI (NaeI)Tango50-10020-500-200-2010050-1001X or 2X0.565°C
PdmI (XmnI)Tango20-5050-1000-200-201000-201X0.565°C
PfeI (TfiI)O0-2020-5010050-10020-5050-1001X or 2X0.165°C
Pfl23II (BsiWI)Tango20-5050-10020-5020-501000-201X0.365°C
PfoITango0-2020-5050-1000-2010050-1001X or 2X0.165°C
Ppu21I (BsaAI)Ppu21I50-100*100*20-50NRNRNRNR0.565°C
PscI (PciI)Tango20-5020-500-200-201000-201X0.265°C
Psp1406I (AclI)Tango10050-1000-2020-501000-201X0.565°C
Psp5II (PpuMI)G0-2010020-5020-5050-1001001X or 2X0.280°C
PstIO50-10050-10010010050-10050-1001X or 2X0.280°C (10u)
PsuI (BstYI)B10020-500-200-2050-1000-201X0.580°C
PsyI (Tth111I)B10050-1000-200-2050-1000-201X0.180°C
PvuIR0-2020-5050-10010050-1001001X or 2X0.280°C (10u)
PvuIIG50-100*10020-5050-10020-50*20-50*1X* or 2X*0.280°C
RsaITango50-10020-500-200-201000-201X0.265°C
RseI (MslI)R0-2050-10050-10010020-501001X or 2X0.565°C
SacISacI50-10020-500-200-2050-10020-501X or 2X0.265°C
SalIO0-200-2010020-500-2050-1002X0.165°C
SatI (Fnu4HI)G20-5010020-5020-5050-10020-501X or 2X0.165°C
ScaIScaI0-200-200-200-200-200-20NR0.380°C (10u)
SchI (MlyI)Tango20-5050-1000-200-201000-201X0.265°C
SdaI (SbfI)SdaINRNR0-200-20NR20-502X0.365°C
SduI (Bsp1286I)SduINR50-100*0-200-20NRNRNR0.365°C
SfaAI (AsiSI)Tango50-1000-200-200-201000-201X0.280°C
SfiIG50-10010020-500-201000-201X0.280°C
SgeISgeI0-200-200-20NRNRNRNR0.065°C
SgrDIR0-200-200-20100NR1002X0.365°C
SgsI (AscI)Tango0-200-200-2050-10010050-1001X or 2X0.165°C
SmaITango50-1000-200-200-201000-201X0.265°C
SmiI (SwaI)O0-200-2010020-500-2020-502X0.165°C
SmoI (SmlI)Tango50-10020-500-2020-5010020-501X or 2X0.280°C
SsiI (AciI)ONR20-5010050-100NR1002X0.565°C
SspDI (KasI)Tango20-500-20NR0-2010020-501X or 2X1.080°C
SspIG20-501000-2050-10010020-501X or 2X0.165°C
TaaI (HpyCH4III)Tango0-200-200-2050-1001001001X or 2X0.2No
TaiI (MaeII)R50-10050-10020-5010010050-1001X or 2X0.3No
TaqITaqI0-2020-5020-5020-5020-5020-501X or 2X0.3No
TasI (Tsp509I)B10050-10020-500-2020-500-201X0.3No
TatITangoNR50-100*0-2020-50100*0-201X*0.2No
TauIB10050-1000-200-2020-500-201X0.9No
Tru1I (MseI)R50-10050-10020-5010050-1001001X or 2X0.2No
TscAI (TspRI)Tango50-10050-10020-5020-5010020-501X or 2X0.2No
Van91I (PflMI)R0-2050-10050-10010020-5050-1001X or 2X0.165°C
VspI (AseI)O0-2050-10010020-501001001X or 2X0.165°C
XagI (EcoNI)R0-2020-5050-10010020-5050-1001X or 2X0.165°C
XapI (ApoI)Tango50-1001000-200-2010020-501X or 2X0.165°C
XbaITango50-10050-10020-500-2010050-1001X or 2X0.165°C
XceI (NspI)Tango50-1000-200-200-201000-201X0.265°C
XhoIR0-2050-10050-10010020-501001X or 2X0.180°C
XmaJI (AvrII)Tango20-5050-10050-10050-10010050-1001X or 2X0.280°C
XmiI (AccI)B1000-200-200-2050-10020-501X or 2X0.165°C