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Thermo Scientific Enzyme | Recommended buffer for 100% activity | Enzyme activity in 5-buffer system buffers, % | Tango buffer for double digestion | Units for overnight incubation, u/μg DNA | Thermal inactivation in 20 min | |||||
---|---|---|---|---|---|---|---|---|---|---|
B (blue) 1X | G (green) 1X | O (orange) 1X | R (red) 1X | Tango (yellow) 1X / 2X | ||||||
AanI (PsiI) | Tango | 50-100 | 50-100 | 0-20 | 0-20 | 100 | 20-50 | 1X or 2X | 0.5 | 65°C |
AarI | AarI +oligo | NR (+oligo) | NR (+oligo) | 0-20 (+oligo) | 0-20 (+oligo) | NR (+oligo) | 50-100 (+oligo) | 2X (+oligo) | 0.9 | 65°C |
AatII | Tango | 50-100 | 20-50 | 0-20 | 0-20 | 100 | 20-50 | 1X or 2X | 0.3 | 65°C |
Acc65I (Asp718I) | O | 0-20 | 20-50 | 100 | 20-50 | 20-50 | 50-100 | 1X or 2X | 0.3 | 65°C |
AdeI (DraIII) | G | 0-20 | 100 | 20-50 | 100 | 100* | 20-50 | 1X* or 2X | 0.5 | No |
AjiI (BmgBI) | AjiI | NR | NR | 20-50* | NR | NR | 20-50* | 2X* | 0.5 | 65°C |
AjuI | R +SAM | 0-20 (+SAM) | 50-100 (+SAM) | 20-50 (+SAM) | 100 (+SAM) | 50-100 (+SAM) | 50-100 (+SAM) | 1X or 2X (+SAM) | 0.5 | 65°C |
AloI | R | 0-20 | 0-20 | 0-20 | 100 | 20-50 | 100 | 1X or 2X | 0.1 | 65°C |
AluI | Tango | 50-100 | 0-20 | 0-20 | 0-20 | 100 | 20-50 | 1X or 2X | 0.1 | 65°C |
Alw21I (BsiHKAI) | O | 0-20 | 20-50 | 100 | 50-100 | 20-50 | 50-100 | 1X or 2X | 0.1 | 65°C |
Alw26I | Tango | 50-100 | 100 | 0-20 | 0-20 | 100 | 100 | 1X or 2X | 0.2 | 65°C |
Alw44I | Tango | 50-100 | 100 | 0-20 | 50-100 | 100 | 50-100 | 1X or 2X | 0.1 | 65°C |
ApaI | B | 100 | 20-50 | 0-20 | 0-20 | 20-50 | 0-20 | 1X | 0.2 | 65°C |
BamHI | BamHI | 20-50* | 100 | 20-50 | 50-100* | 100* | 50-100 | 1X* or 2X | 0.5 | 80°C (10u) |
BauI (BssSI) | Tango | 0-20 | 50-100 | 0-20 | 50-100 | 100 | 50-100 | 1X or 2X | 0.2 | 65°C |
BclI | G | 20-50 | 100 | 20-50 | 20-50 | 100* | 100 | 1X* or 2X | 0.1 | 80°C (10u) |
BcnI (NciI) | Tango | 20-50 | 50-100 | 50-100 | 50-100 | 100 | 50-100 | 1X or 2X | 0.2 | 65°C |
BcuI (SpeI) | Tango | 50-100 | 50-100 | 0-20 | 20-50 | 100 | 0-20 | 1X | 0.5 | No |
BfmI (SfcI) | Tango | 0-20 | 50-100 | 0-20 | 0-20 | 100 | 20-50 | 1X or 2X | 0.9 | 65°C |
BfuI (BciVI) | BfuI | NR | NR | 0-20 | 0-20 | NR | 50-100* | 2X* | 0.9 | 80°C |
BglI | O | 0-20 | 50-100 | 100 | 100 | 0-20 | 100 | 2X | 0.1 | 65°C |
BglII | O | 0-20 | 20-50 | 100 | 50-100 | 0-20 | 100 | 2X | 0.1 | No |
Bme1390I (ScrFI) | O | 20-50 | 50-100 | 100 | 50-100 | 50-100 | 50-100 | 1X or 2X | 0.2 | 80°C |
BoxI (PshAI) | Tango | 0-20 | 0-20 | 0-20 | 20-50 | 100 | 20-50 | 1X or 2X | 0.5 | 80°C |
BpiI (BbsI) | G | 20-50 | 100 | 50-100 | 50-100 | 50-100 | 50-100 | 1X or 2X | 0.3 | 65°C |
BplI | Tango | 0-20 (+SAM) | 20-50 (+SAM) | 0-20 (+SAM) | 0-20 (+SAM) | 100 (+SAM) | 20-50 (+SAM) | 1X (+SAM) | 0.3 | 65°C |
Bpu10I | Bpu10I | 0-20 | 20-50* | 50-100* | 100* | 50-100* | 100* | 1X* or 2X* | 0.2 | 80°C |
Bpu1102I (BlpI) | Tango | 50-100 | 50-100 | 20-50 | 20-50 | 100 | 20-50 | 1X or 2X | 0.1 | 80°C |
BseDI (BsaJI) | Tango | 50-100 | 20-50 | 0-20 | 0-20 | 100 | 50-100 | 1X or 2X | 0.2 | 80°C |
BseGI (BtsCI) | Tango | 20-50 | 50-100 | 20-50 | 20-50 | 100 | 20-50 | 1X or 2X | 0.2 | 80°C |
BseJI (BsaBI) | O | NR | 100* | 100 | NR | NR | 100* | 2X* | 0.1 | No |
BseLI (BslI) | Tango | 20-50 | 100 | 0-20 | 20-50 | 100 | 50-100 | 1X or 2X | 0.1 | No |
BseMI (BsrDI) | R | 0-20 | 20-50 | 0-20 | 100 | 50-100 | 50-100 | 1X or 2X | 0.3 | 80°C |
BseMII (BspCNI) | Tango | 50-100 (+SAM) | 50-100 (+SAM) | 50-100 (+SAM) | 50-100 (+SAM) | 100 (+SAM) | 50-100 (+SAM) | 1X or 2X (+SAM) | 0.5 | 80°C |
BseNI (BsrI) | B | 100 | 20-50 | 0-20 | 0-20 | 50-100 | 20-50 | 1X or 2X | 0.1 | 80°C |
BseSI (Bme1580I) | G | 20-50 | 100 | 0-20 | 20-50 | 50-100 | 0-20 | 1X | 0.1 | 80°C (10u) |
BseXI (BbvI) | BseXI | NR | NR | NR | NR | NR | NR | NR | 0.3 | 80°C |
Bsh1236I (BstUI) | R | 0-20 | 0-20 | 50-100 | 100 | 20-50 | 50-100 | 1X or 2X | 0.1 | 65°C |
Bsh1285I (BsiEI) | G | 20-50 | 100 | 20-50 | 0-20 | 0-20 | 20-50 | 2X | 0.2 | 65°C |
BshNI (BanI) | O | 0-20 | 20-50 | 100 | 50-100 | 0-20 | 100 | 2X | 0.3 | 65°C |
BshTI (AgeI) | O | 0-20 | 20-50 | 100 | 50-100 | 20-50 | 20-50 | 1X or 2X | 0.1 | 65°C |
Bsp119I (BstBI) | Tango | 20-50 | 0-20 | 0-20 | 0-20 | 100 | 100 | 1X or 2X | 0.1 | 80°C |
Bsp120I (PspOMI) | B | 100 | 20-50 | 0-20 | 20-50 | 50-100 | 0-20 | 1X | 0.1 | 80°C |
Bsp1407I (BsrGI) | Tango | 0-20 | 20-50 | 0-20 | 20-50 | 100 | 50-100 | 1X or 2X | 0.1 | 65°C |
Bsp143I (Sau3AI) | Bsp143I | 20-50 | 20-50 | 0-20 | 0-20 | 50-100 | 20-50 | 1X or 2X | 0.1 | 65°C |
Bsp68I (NruI) | O | 0-20 | 20-50 | 100 | 50-100 | 20-50 | 50-100 | 1X or 2X | 0.1 | 65°C |
BspLI (NlaIV) | Tango | 50-100 | 50-100 | 0-20 | 20-50 | 100 | 20-50 | 1X or 2X | 0.3 | 65°C |
BspOI (BmtI) | O | 0-20 | 0-20 | 100 | 100 | 0-20 | 20-50 | 2X | 0.2 | 80°C |
BspPI (AlwI) | Tango | 20-50 | 20-50 | 0-20 | 0-20 | 100 | 0-20 | 1X | 0.5 | 80°C |
BspTI (AflII) | O | 0-20 | 0-20 | 100 | 20-50 | 0-20 | 50-100 | 2X | 0.1 | 65°C |
Bst1107I (BstZ17I) | O | 20-50 | 50-100 | 100 | 100 | 20-50 | 100 | 1X or 2X | 0.1 | 65°C |
BstXI | O | 20-50 | 100 | 100 | 50-100 | 50-100 | 100 | 1X or 2X | 0.1 | 65°C |
Bsu15I (ClaI) | Tango | 20-50 | 20-50 | 20-50 | 20-50 | 100 | 20-50 | 1X or 2X | 0.1 | 65°C |
BsuRI (HaeIII) | R | 0-20 | 0-20 | 0-20 | 100 | 50-100 | 100 | 1X or 2X | 0.1 | 80°C |
BveI (BspMI) | O +oligo | 0-20 (+oligo) | 20-50 (+oligo) | 100 (+oligo) | 20-50 (+oligo) | 50-100 (+oligo) | 100 (+oligo) | 1X or 2X (+oligo) | 0.2 | 65°C |
CaiI (AlwNI) | Tango | 20-50 | 20-50 | 20-50 | 50-100 | 100 | 50-100 | 1X or 2X | 0.2 | 65°C |
Cfr10I (BsrFI) | Cfr10I | 0-20 | 20-50 | 20-50 | 50-100 | 20-50 | 50-100 | 1X or 2X | 0.1 | No |
Cfr13I (Sau96I) | Tango | 50-100 | 50-100 | 20-50 | 20-50 | 100 | 20-50 | 1X or 2X | 0.3 | 65°C |
Cfr42I (SacII) | B | 100 | 50-100 | 0-20 | 0-20 | 50-100 | 0-20 | 1X | 0.1 | 65°C |
Cfr9I (XmaI) | Cfr9I | 0-20 | 0-20 | 0-20 | 0-20 | 20-50 | 0-20 | 1X | 0.2 | 65°C |
CpoI (RsrII) | Tango | 20-50 | 50-100 | 50-100 | 20-50 | 100 | 50-100 | 1X or 2X | 0.5 | 65°C |
CseI (HgaI) | R | NR | 50-100* | 50-100 | 100 | 100* | 50-100 | 1X* or 2X | 0.5 | 80°C (10u) |
Csp6I (CviQI) | B | 100 | 50-100 | 0-20 | 0-20 | 50-100 | 0-20 | 1X | 0.1 | 65°C |
DpnI | Tango | 100 | 100 | 50-100 | 50-100 | 100 | 50-100 | 1X or 2X | 0.1 | 80°C |
DraI | Tango | 50-100 | 50-100 | 20-50 | 20-50 | 100 | 50-100 | 1X or 2X | 0.1 | 65°C |
Eam1104I (EarI) | Tango | 50-100 | 50-100 | 0-20 | 0-20 | 100 | 0-20 | 1X | 0.5 | 65°C |
Eam1105I (AhdI) | Eam1105I | 20-50 | 50-100 | 0-20 | 0-20 | 50-100 | 20-50 | 1X or 2X | 0.1 | 65°C |
Ecl136II (EcoICRI) | Ecl136II | 50-100 | 20-50 | 0-20 | 0-20 | 50-100 | 0-20 | 1X | 0.2 | 65°C |
Eco105I (SnaBI) | Tango | 100* | 50-100 | 0-20 | 0-20 | 100 | 0-20 | 1X | 0.5 | 65°C |
Eco130I (StyI) | O | 0-20 | 20-50 | 100 | 50-100 | 50-100 | 100 | 1X or 2X | 0.2 | 65°C |
Eco147I (StuI) | B | 100 | 50-100 | 20-50 | 20-50 | 50-100 | 0-20 | 1X | 0.1 | 80°C |
Eco24I (BanII) | Tango | 50-100 | 50-100 | 20-50 | 20-50 | 100 | 0-20 | 1X | 0.2 | 65°C |
Eco31I (BsaI) | G | 50-100 | 100 | 0-20 | 0-20 | 50-100 | 20-50 | 1X or 2X | 0.3 | 65°C |
Eco32I (EcoRV) | R | 0-20 | 50-100 | 50-100 | 100 | 20-50 | 100 | 1X or 2X | 0.1 | 65°C |
Eco47I (AvaII) | R | 0-20 | 50-100 | 50-100 | 100 | 50-100 | 50-100 | 1X or 2X | 0.3 | 65°C |
Eco47III (AfeI) | O | 0-20 | 20-50 | 100 | 100 | 50-100 | 100 | 1X or 2X | 0.1 | 80°C |
Eco52I (EagI) | Eco52I | 0-20 | 0-20 | 0-20 | 20-50 | 0-20 | 20-50 | 2X | 0.2 | 65°C |
Eco57I (AcuI) | G +SAM | 100 (+SAM) | 100 (+SAM) | 20-50 (+SAM) | 20-50 (+SAM) | 50-100 (+SAM) | 50-100 (+SAM) | 1X or 2X (+SAM) | 0.9 | 65°C |
Eco72I (PmlI) | Tango | NR | NR | 0-20 | 0-20 | 100 | 20-50 | 1X or 2X | 0.5 | 65°C |
Eco81I (Bsu36I) | Tango | 50-100 | 100 | 0-20 | 0-20 | 100 | 0-20 | 1X | 0.1 | 80°C |
Eco88I (AvaI) | Tango | 100 | 50-100 | 0-20 | 0-20 | 100 | 20-50 | 1X or 2X | 0.2 | 65°C |
Eco91I (BstEII) | O | 20-50 | 20-50 | 100 | 50-100 | NR | 100 | 2X | 0.1 | 65°C |
EcoO109I (DraII) | Tango | 50-100 | 20-50 | 20-50 | 20-50 | 100 | 100 | 1X or 2X | 0.2 | 65°C |
EcoRI | EcoRI | 0-20 | NR | 100 | 100* | NR | 100 | 2X | 0.2 | 65°C |
EcoRII | O | 20-50 | 50-100 | 100 | 50-100 | 20-50 | 50-100 | 1X or 2X | 0.1 | 80°C |
EheI (SfoI) | Tango | 20-50 | 50-100 | 0-20 | 0-20 | 100 | 20-50 | 1X or 2X | 0.9 | 65°C |
Esp3I (BsmBI) | Tango | 100 (+DTT) | 20-50 (+DTT) | 0-20 (+DTT) | 0-20 (+DTT) | 100 (+DDT) | 0-20 (+DTT) | 1X (+DDT) | 0.2 | 65°C |
FaqI (BsmFI) | Tango | 20-50 (+SAM) | 20-50 (+SAM) | 0-20 (+SAM) | 0-20 (+SAM) | 100 (+SAM) | 50-100 (+SAM) | 1X (+SAM) | 0.9 | 80°C |
FspAI | O | 0-20 | 0-20 | 100 | 50-100 | 0-20 | 50-100 | 2X | 0.2 | 65°C |
FspBI (BfaI) | Tango | 50-100 | 20-50 | 0-20 | 0-20 | 100 | 0-20 | 1X | 0.3 | 65°C |
GsuI (BpmI) | B | 100 | 50-100 | 0-20 | 20-50 | 100 | 50-100 | 1X or 2X | 0.9 | 65°C |
HhaI | Tango | 50-100 | 50-100 | 20-50 | 20-50 | 100 | 20-50 | 1X or 2X | 0.1 | 80°C |
Hin1I (BsaHI) | G | 20-50 | 100 | 20-50 | 20-50 | 20-50 | 20-50 | 1X or 2X | 0.1 | 65°C |
Hin1II (NlaIII) | G | 50-100 | 100 | 20-50 | 50-100 | 50-100 | 50-100 | 1X or 2X | 0.3 | 65°C |
Hin6I (HinP1I) | Tango | 50-100 | 50-100 | 20-50 | 20-50 | 100 | 50-100 | 1X or 2X | 0.1 | 65°C |
HincII (HindII) | Tango | 50-100 | 50-100 | 20-50 | 50-100 | 100 | 50-100 | 1X or 2X | 0.1 | 65°C |
HindIII | R | 0-20 | 20-50 | 0-20 | 100 | 50-100 | 50-100 | 1X or 2X | 0.1 | 80°C |
HinfI | R | 0-20 | 20-50 | 50-100 | 100 | 50-100 | 50-100 | 1X or 2X | 0.1 | 65°C |
HpaII | Tango | 50-100 | 50-100 | 0-20 | 20-50 | 100 | 20-50 | 1X or 2X | 0.1 | 65°C |
HphI | B | 100 | 0-20 | 0-20 | 0-20 | 20-50 | 0-20 | 1X | 0.1 | 65°C |
Hpy8I (MjaIV) | Tango | 50-100 | 50-100 | 0-20 | 20-50 | 100 | 50-100 | 1X or 2X | 0.1 | 80°C |
HpyF10VI (MwoI) | Tango | 0-20 | 0-20 | 0-20 | 0-20 | 100 | 50-100 | 1X or 2X | 0.1 | 80°C |
HpyF3I (DdeI) | Tango | 20-50 | 20-50 | 20-50 | 20-50 | 100 | 50-100 | 1X or 2X | 0.2 | 65°C |
I-SceI | Tango | 50-100 | 50-100 | 50-100 | 50-100 | 100 | 50-100 | 1X or 2X | 0.9 | 65°C |
Kpn2I (BspEI) | Tango | 50-100 | 50-100 | 0-20 | 20-50 | 100 | 50-100 | 1X or 2X | 0.1 | 80°C |
KpnI | KpnI | 20-50 | 0-20 | 0-20 | 0-20 | 20-50 | 0-20 | 1X | 0.2 | 80°C |
KspAI (HpaI) | B | 100 | 50-100* | 20-50 | 20-50 | 100* | 50-100 | 1X* or 2X* | 0.5 | 65°C |
LguI (SapI) | Tango | 20-50 | 50-100 | 20-50 | 20-50 | 100 | 20-50 | 1X or 2X | 0.5 | 65°C |
Lsp1109I (BbvI) | Lsp1109I | 0-20 | 20-50* | 50-100* | 100 | 20-50* | 20-50* | 1X* or 2X | 0.5 | 65°C |
LweI (SfaNI) | Tango | 0-20 | 0-20 | 0-20 | 20-50 | 100 | 20-50 | 1X or 2X | 0.2 | 65°C |
MauBI | Tango | 0-20 | 0-20 | 0-20 | 0-20 | 100 | 0-20 | 1X | 0.1 | 65°C |
MbiI (BsrBI) | Tango | 20-50 | 100 | 0-20 | 20-50 | 100 | 20-50 | 1X or 2X | 0.2 | 65°C |
MboI | R | 50-100 | 50-100 | 50-100 | 100 | 50-100 | 100 | 1X or 2X | 0.1 | 65°C |
MboII | B | 100 | 50-100 | 20-50 | 0-20 | 50-100 | 20-50 | 1X or 2X | 0.9 | 65°C |
MlsI (MscI) | R | 0-20 | 20-50 | 0-20 | 100 | 20-50 | 50-100 | 1X or 2X | 0.5 | 65°C |
MluI | R | 0-20 | 20-50 | 50-100 | 100 | 20-50 | 50-100 | 1X or 2X | 0.1 | 65°C |
MnlI | G | 50-100 | 100 | 20-50 | 20-50 | 20-50 | 20-50 | 1X or 2X | 0.5 | 65°C |
Mph1103I (NsiI) | R | 0-20 | 50-100 | 20-50 | 100 | 50-100 | 50-100 | 1X or 2X | 0.3 | 65°C |
MreI (Sse232I) | G | 0-20 | 100 | 0-20 | 0-20 | 50-100 | 0-20 | 1X | 0.5 | 80°C |
MspI (HpaII) | Tango | 50-100 | 50-100 | 0-20 | 0-20 | 100 | 50-100 | 1X or 2X | 0.3 | 65°C |
MssI (PmeI) | B | 100 | 0-20 | 0-20 | 0-20 | 20-50 | 0-20 | 1X | 0.5 | 65°C |
MunI (MfeI) | G | 100 | 100 | 0-20 | 0-20 | 100 | 0-20 | 1X | 0.1 | 65°C |
Mva1269I (BsmI) | R | 0-20 | 20-50 | 50-100 | 100 | 0-20 | 50-100 | 2X | 0.1 | 65°C |
MvaI (BstNI) | R | 20-50 | 20-50 | 50-100 | 100 | 20-50* | 100 | 1X* or 2X | 0.1 | 80°C (10u) |
Nb.Bpu10I | R | 0-20 | 20-50 | 20-50 | 100 | 20-50 | 50-100 | 1X or 2X | 0.3 | 80°C |
Nb.Mva1269I | O | 0-20 | 20-50 | 100 | 100 | 20-50 | 50-100 | 1X or 2X | 0.3 | 80°C |
NcoI | Tango | 20-50 | 20-50 | 20-50 | 50-100 | 100 | 100 | 1X or 2X | 0.1 | 65°C |
NdeI | O | 0-20 | 0-20 | 100 | 50-100 | 0-20 | 50-100 | 2X | 0.3 | 65°C |
NheI | Tango | 100 | 20-50 | 0-20 | 0-20 | 100 | 0-20 | 1X | 0.2 | 65°C |
NmuCI (Tsp45I) | R | 0-20 | 20-50 | 50-100 | 100 | 20-50 | 50-100 | 1X or 2X | 0.1 | 65°C |
NotI | O | 0-20 | 20-50 | 100 | 20-50 | 0-20 | 20-50 | 2X | 0.1 | 65°C |
NsbI (FspI) | Tango | 20-50 | 50-100 | 0-20 | 20-50 | 100 | 20-50 | 1X or 2X | 0.1 | 65°C |
Nt.Bpu10I | R | 0-20 | 0-20 | 100 | 100 | 0-20 | 50-100 | 2X | 0.3 | 80°C |
OliI (AleI) | R | 0-20 | 0-20 | 0-20 | 100 | 0-20 | 50-100 | 2X | 0.2 | 65°C |
PacI | PacI | 20-50 | 20-50 | 0-20 | 0-20 | 0-20 | 0-20 | NR | 0.3 | 65°C |
PaeI (SphI) | B | 100 | 50-100 | 0-20 | 0-20 | 50-100 | 0-20 | 1X | 0.1 | 65°C |
PagI (BspHI) | O | 0-20 | 50-100 | 100 | NR | NR | NR | NR | 0.2 | 80°C |
PasI | PasI | NR | NR | NR | NR | NR | NR | NR | 0.2 | 80°C |
PauI (BssHII) | R | 0-20 | 0-20 | 100 | 100 | 0-20 | 100 | 2X | 0.2 | 80°C |
PdiI (NaeI) | Tango | 50-100 | 20-50 | 0-20 | 0-20 | 100 | 50-100 | 1X or 2X | 0.5 | 65°C |
PdmI (XmnI) | Tango | 20-50 | 50-100 | 0-20 | 0-20 | 100 | 0-20 | 1X | 0.5 | 65°C |
PfeI (TfiI) | O | 0-20 | 20-50 | 100 | 50-100 | 20-50 | 50-100 | 1X or 2X | 0.1 | 65°C |
Pfl23II (BsiWI) | Tango | 20-50 | 50-100 | 20-50 | 20-50 | 100 | 0-20 | 1X | 0.3 | 65°C |
PfoI | Tango | 0-20 | 20-50 | 50-100 | 0-20 | 100 | 50-100 | 1X or 2X | 0.1 | 65°C |
Ppu21I (BsaAI) | Ppu21I | 50-100* | 100* | 20-50 | NR | NR | NR | NR | 0.5 | 65°C |
PscI (PciI) | Tango | 20-50 | 20-50 | 0-20 | 0-20 | 100 | 0-20 | 1X | 0.2 | 65°C |
Psp1406I (AclI) | Tango | 100 | 50-100 | 0-20 | 20-50 | 100 | 0-20 | 1X | 0.5 | 65°C |
Psp5II (PpuMI) | G | 0-20 | 100 | 20-50 | 20-50 | 50-100 | 100 | 1X or 2X | 0.2 | 80°C |
PstI | O | 50-100 | 50-100 | 100 | 100 | 50-100 | 50-100 | 1X or 2X | 0.2 | 80°C (10u) |
PsuI (BstYI) | B | 100 | 20-50 | 0-20 | 0-20 | 50-100 | 0-20 | 1X | 0.5 | 80°C |
PsyI (Tth111I) | B | 100 | 50-100 | 0-20 | 0-20 | 50-100 | 0-20 | 1X | 0.1 | 80°C |
PvuI | R | 0-20 | 20-50 | 50-100 | 100 | 50-100 | 100 | 1X or 2X | 0.2 | 80°C (10u) |
PvuII | G | 50-100* | 100 | 20-50 | 50-100 | 20-50* | 20-50* | 1X* or 2X* | 0.2 | 80°C |
RsaI | Tango | 50-100 | 20-50 | 0-20 | 0-20 | 100 | 0-20 | 1X | 0.2 | 65°C |
RseI (MslI) | R | 0-20 | 50-100 | 50-100 | 100 | 20-50 | 100 | 1X or 2X | 0.5 | 65°C |
SacI | SacI | 50-100 | 20-50 | 0-20 | 0-20 | 50-100 | 20-50 | 1X or 2X | 0.2 | 65°C |
SalI | O | 0-20 | 0-20 | 100 | 20-50 | 0-20 | 50-100 | 2X | 0.1 | 65°C |
SatI (Fnu4HI) | G | 20-50 | 100 | 20-50 | 20-50 | 50-100 | 20-50 | 1X or 2X | 0.1 | 65°C |
ScaI | ScaI | 0-20 | 0-20 | 0-20 | 0-20 | 0-20 | 0-20 | NR | 0.3 | 80°C (10u) |
SchI (MlyI) | Tango | 20-50 | 50-100 | 0-20 | 0-20 | 100 | 0-20 | 1X | 0.2 | 65°C |
SdaI (SbfI) | SdaI | NR | NR | 0-20 | 0-20 | NR | 20-50 | 2X | 0.3 | 65°C |
SduI (Bsp1286I) | SduI | NR | 50-100* | 0-20 | 0-20 | NR | NR | NR | 0.3 | 65°C |
SfaAI (AsiSI) | Tango | 50-100 | 0-20 | 0-20 | 0-20 | 100 | 0-20 | 1X | 0.2 | 80°C |
SfiI | G | 50-100 | 100 | 20-50 | 0-20 | 100 | 0-20 | 1X | 0.2 | 80°C |
SgeI | SgeI | 0-20 | 0-20 | 0-20 | NR | NR | NR | NR | 0.0 | 65°C |
SgrDI | R | 0-20 | 0-20 | 0-20 | 100 | NR | 100 | 2X | 0.3 | 65°C |
SgsI (AscI) | Tango | 0-20 | 0-20 | 0-20 | 50-100 | 100 | 50-100 | 1X or 2X | 0.1 | 65°C |
SmaI | Tango | 50-100 | 0-20 | 0-20 | 0-20 | 100 | 0-20 | 1X | 0.2 | 65°C |
SmiI (SwaI) | O | 0-20 | 0-20 | 100 | 20-50 | 0-20 | 20-50 | 2X | 0.1 | 65°C |
SmoI (SmlI) | Tango | 50-100 | 20-50 | 0-20 | 20-50 | 100 | 20-50 | 1X or 2X | 0.2 | 80°C |
SsiI (AciI) | O | NR | 20-50 | 100 | 50-100 | NR | 100 | 2X | 0.5 | 65°C |
SspDI (KasI) | Tango | 20-50 | 0-20 | NR | 0-20 | 100 | 20-50 | 1X or 2X | 1.0 | 80°C |
SspI | G | 20-50 | 100 | 0-20 | 50-100 | 100 | 20-50 | 1X or 2X | 0.1 | 65°C |
TaaI (HpyCH4III) | Tango | 0-20 | 0-20 | 0-20 | 50-100 | 100 | 100 | 1X or 2X | 0.2 | No |
TaiI (MaeII) | R | 50-100 | 50-100 | 20-50 | 100 | 100 | 50-100 | 1X or 2X | 0.3 | No |
TaqI | TaqI | 0-20 | 20-50 | 20-50 | 20-50 | 20-50 | 20-50 | 1X or 2X | 0.3 | No |
TasI (Tsp509I) | B | 100 | 50-100 | 20-50 | 0-20 | 20-50 | 0-20 | 1X | 0.3 | No |
TatI | Tango | NR | 50-100* | 0-20 | 20-50 | 100* | 0-20 | 1X* | 0.2 | No |
TauI | B | 100 | 50-100 | 0-20 | 0-20 | 20-50 | 0-20 | 1X | 0.9 | No |
Tru1I (MseI) | R | 50-100 | 50-100 | 20-50 | 100 | 50-100 | 100 | 1X or 2X | 0.2 | No |
TscAI (TspRI) | Tango | 50-100 | 50-100 | 20-50 | 20-50 | 100 | 20-50 | 1X or 2X | 0.2 | No |
Van91I (PflMI) | R | 0-20 | 50-100 | 50-100 | 100 | 20-50 | 50-100 | 1X or 2X | 0.1 | 65°C |
VspI (AseI) | O | 0-20 | 50-100 | 100 | 20-50 | 100 | 100 | 1X or 2X | 0.1 | 65°C |
XagI (EcoNI) | R | 0-20 | 20-50 | 50-100 | 100 | 20-50 | 50-100 | 1X or 2X | 0.1 | 65°C |
XapI (ApoI) | Tango | 50-100 | 100 | 0-20 | 0-20 | 100 | 20-50 | 1X or 2X | 0.1 | 65°C |
XbaI | Tango | 50-100 | 50-100 | 20-50 | 0-20 | 100 | 50-100 | 1X or 2X | 0.1 | 65°C |
XceI (NspI) | Tango | 50-100 | 0-20 | 0-20 | 0-20 | 100 | 0-20 | 1X | 0.2 | 65°C |
XhoI | R | 0-20 | 50-100 | 50-100 | 100 | 20-50 | 100 | 1X or 2X | 0.1 | 80°C |
XmaJI (AvrII) | Tango | 20-50 | 50-100 | 50-100 | 50-100 | 100 | 50-100 | 1X or 2X | 0.2 | 80°C |
XmiI (AccI) | B | 100 | 0-20 | 0-20 | 0-20 | 50-100 | 20-50 | 1X or 2X | 0.1 | 65°C |