Advancing SRM-based Proteomics for Detection and Quantitation of Histone Modifications in Scarce Clinical Specimens

In this webinar, strategies for improving PTM detection and quantitation in scarce clinical samples, and clinical research applications of SRM-based assays will be presented.

Analysis of histone modifications using existing mass spectrometry (MS)-based methods requires large numbers of cells, however for some clinical specimens, it is extremely difficult to obtain a large number of cells. An action plan was outlined to establish and validate a full pipeline to quantitate all major and rare histone modifications from 106 down to 103 cells. To accomplish this feat, a two-pronged approach was proposed using two different MS strategies: full-length histone analysis (top-down) and triple-quadrupole based tryptic peptide quantitation (bottom-up). In this webinar, current and future technology improvements that will significantly improve the limits of detection and quantitation of existing SRM-based bottom-up approach are presented.